The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen compatible cell lines.
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The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has been developed to convert mammalian cell lines into AequoScreen® compatible cell lines. It is a powerful tool which enables the rapid development and analysis of cell lines, already expressing a recombinant or endogenous G-protein coupled receptor of choice, to a luminescence readout. The aequorin plasmid targets cells expressing any protein that modulates intracellular calcium concentration (e.g., GPCRs within the Gq family or GPCRs that couple via a promiscuous G-Protein / chimera).
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|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|Product Brand Name||AequoScreen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||10 µg|
|Resource Type||File Name||File Format|
|Application Note||AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines||PDF 607 KB|
|Poster||Running an Aequorin Cell-Based Luminescent Assay on the FLIPR TETRA||PDF 270 KB|
|Flyer||Aequorin FLIPR||PDF 1 MB|