Label-free Detection

Characterize cellular signaling mechanisms more completely and better understand the complexity of multiple signaling pathways more efficiently with label-free technology. Perform highly sensitive label-free biochemical binding assays and study even difficult targets or weak biological interactions.

With label-free technology you can generate more information-rich and physiologically relevant data from your cellular and biochemical assays for a better understanding.

Make Better Decisions Sooner

From target confirmation to hit-to-lead and lead optimization, label-free detection delivers a unique orthogonal perspective, providing:

  • Rich information about both cellular and biochemical assays
  • Pathway unbiased analysis of both endogenously and recombinantly expressed targets
  • Non-invasive, more physiologically relevant data
  • Ability to study difficult targets (e.g. Gi-coupled receptors) or weak biological interactions

The EnSpire® Multimode Plate Reader offers established Corning® Epic® label-free technology in addition to industry-leading labeled technologies. The Epic® technology has been proven across a wide range of applications using recombinantly and endogenously expressed targets, including difficult-to-screen GPCRs as well as receptor tyrosine kinases, ion channels, protein:protein interactions, protein:small molecule interactions and binding strength KD.

Download Technical Note Comparing Performance of the EnSpire® Reader with the Corning Epic System

Read the Poster on Label-free Analysis of GABA Receptor in iPSC Derived Neurons

Powerful Performance for GPCR Assays

Click the target below to view the assay results.

EnSpire
Label-free
Epic®
Label-free
EnSpire
Labeled Assay**
TargetHost
Cell
Culture
Conditions
Agonist
EC50**
Anta-gonist
IC50**
Agonist
EC50**
Anta-gonist
IC50
Agonist
EC50
Anta-gonist
IC50
β2AR (GS)A431
(Endogenous)
Passaged0.036.20.026.01**1.8
β2AR (GS)CH0-K1
(Recombinant)
Frozen0.010.3--11.4
Opioid Mu (Gi)CH0-K1
(Recombinant)
Frozen0.9430.5352.30.03
A1 (Gi)HEK293
(Endogenous)
Passaged4735100153--
M1 (Gq)HEK293
(Endogenous)
Passaged5,9002.25,0002.0--
M1 (Gq)CH0-K1
(Recombinant)
Passaged1776.3--8514

*Potency in nM   **Labeled Assays:  AlphaScreen cAMP (β2AR (GS), Opioid Mu (Gi), Ca2+ Flux Assay (M1).
Label-free pathway-independent response is demonstrated across GPCR pathways including more difficult Gi receptors.
Pharmacological response was independent of whether cells were endogenous or recombinant or whether freshly passaged or frozen.

Orthogonal Multimode Detection

Only the EnSpire benchtop plate reader detects labeled and label-free assays. For truly versatile detection, the EnSpire label-free platform includes optional patented Alpha technology, ultra-sensitive luminescence, fluorescence intensity and absorbance detection.

Microplates for Every Application

Choose from a wide variety of application-focused microplates for every throughput need. PerkinElmer offers label-free enabled microplates the largest selection of validated GPCR cell lines and over 150 ready-to-use frozen cells.

Label-free Automation Solutions

As a complement to the EnSpire plate reader, our range of automated liquid handling systems provides reproducible and consistent results throughout your assay development process. JANUS® Automated Workstation can support your assay development needs as a standalone instrument or integrated with other devices to achieve your desired level of walkaway automation.

agonsit antagonist_labelfree graph.jpg

β2 agonist and antagonist dose response curves using AlphaScreen® cAMP confirm the β2 pharmacology from the EnSpire label-free platform (384-well, 10,000 cells/well).

cell viability_labelfree graph.jpg

Sequential assays can be performed following a label-free assay to save time and resource, as demonstrated with ATPLite® luminescence which measures cell viability. The results show that label-free analysis has no effect on cell viability.