FMT: Fluorescence Tomography

In vivo imaging research

PerkinElmer has pioneered fluorescence-based Tomography for in vivo imaging research. Imaging systems based on PerkinElmer's proprietary FMT technology provide non-invasive, whole body, deep tissue imaging in small animal models and generate 3D, information-rich results. These systems are used for research in oncology as well as inflammatory, pulmonary, cardiovascular and skeletal disease. Biological targets and pathways can be monitored and quantified in real time - giving a deeper understanding of the biology underlying disease mechanisms and therapeutic response.

Image: Sagittal, transverse and coronal reconstructions of a mouse prostate scanned in vivo using AngioSense. Courtesy of Drs. H. Alencar and U. Mahmood, Massachusetts General Hospital.

Image: Absolute quantification based on robust data even within deep tissue

Quantitative imaging data

Fluorescence quantification in deep tissue.


Image: ROI Analysis yields 0.97nmol estimate from tomographic reconstruction after direct injection of 1 nmol AngioSense

Linearity of measurement. Quantifiable measurement of fluorochrome concentration at depth, exhibits strict linearity in vivo over a broad range of biologically-relevant fluorescence agent concentrations.

concentration_diagram.gif concentration_diagram_2.gif

Image: Strict linearity over a broad range of biologically relevant fluorochrome concentrations

fluorochrome_concentrations.jpgResin phantoms with AngioSense 680 and AngioSense 750 at 2.5 mm depth from front surface. Phantoms were made of an optically clear polyester resin cast in 60mm x 60mm x 15mm blocks with a dispersion of TiO2 and ink to match absorption and scattering properties of mouse tissue.