In this tutorial, one of our Application Specialists demonstrates how to use the Colocalization View in Volocity to perform a colocalization analysis. You will learn how to set thresholds for channels and generate Thresholded and Global Pearson’s Correlation Coefficient (PCC) statistics which can be stored in table format within Volocity and exported.
Download the tutorial as a PDF document
Visit the Volocity Quantitation page
For more information on how the colocalization statistics used in Volocity can bring increased clarity to your colocalization studies, please see the following paper which was published in Microscopy and Microanalysis. This paper was co-authored by a PerkinElmer Application Specialist, Software Developer, and colleagues at the University of Ghent, Belgium:
Barlow et al. Colocalization Analysis in Fluorescence Micrographs: Verification of a More Accurate Calculation of Pearson's Correlation Coefficient. Microscopy and Microanalysis 16: 710-724 (2010).