The UltraVIEW VoX is our complete spinning disk confocal system designed to meet the challenges of your live cell imaging. Coupled with our photokinesis unit we bring you a complete FRAP solution from acquisition to analysis.
Video: EB3-labeled microtubules in retinal epithelial cells. The movie shows fluorescence loss in the central cell due to repeated photobleaching (FLIP experiment) of the microtubule organization centre (MOC). Movie courtesy of the Light Microscopy Center, ETH Zurich.
UltraVIEW PhotoKinesis device for FRAP
The photokinesis device is essential to performing experiments requiring the targeted illumination of regions or structures by directing high intensity laser light to those locations in the field of view.
A high speed optical switch directs any one or combination of the laser lines from the Modular Laser System directly to the photokinesis device. Precise, high speed galvanometers in the photokinesis device direct the focused beam to user specified regions or spots for bleaching. During confocal imaging, the photokinesis device features a straight beam path which minimises light loss.
The photokinesis device is fully integrated with UltraVIEW VoX, providing you maximum accuracy and reliability.
Volocity for FRAP
Volocity is our advanced 3D–4D imaging software which includes a range of easy-to-use tools complete for analysis and publication of your FRAP data from images acquired on the UltraVIEW VoX. The duration and speed of pre-bleach, bleach, and post-bleach phases can all be scheduled into FRAP acquisition protocols. Multiple bleach phases can be scheduled for FLIP experiments. The total flexibility available offers numerous targeted illumination workflows. Volocity allows you complete freedom over bleach ROI number, size, shape and position.
FRAP data is displayed in Volocity in the form of a chart after fitting the data to a choice of recovery models. Not only is the data charted, but Volocity also calculates and displays the important values for the mobile fraction (Fm), the immobile fraction (Fi), the diffusion co-efficient and the T 1/2. The chart can be exported for inclusion in reports, along with the image data allowing you to provide a full set of high quality publishable data from one platform.
- Accurate timing
- Choice of regions of interest
- Online analysis with choice of FRAP algorithms
- Flexible acquisition