Movie showing a live cell sequence of an RPE-1 prophase nucleus (0 min) through mitosis and into G1 (150 min), acquired on the UltraVIEW® VoX with Volocity Acquisition. Courtesy of Professor Thomas Cremer and Dr Hilmar Strickfaden, Ludwig-Maximilians-University.Read more about this research.
Volocity, the power behind the UltraVIEW VoX system
Volocity Acquisition is designed to drive the UltraVIEW® VoX 3D Live Cell Imaging System and provides high speed image capture on Mac or Windows OS. It is easy to use and features a powerful range of tools.
Flexibility and ease-of-use for faster results
- Easily build experimental protocols, whether for simple 2D capture or multi-channel experiments
- Add time-lapse, motorised XY stage control for multi-point experiments, stitch multiple fields, acquire 2D reference images within 3D stacks within the same interface
- Build high performance systems with options for dual camera simultaneous acquisition for the fastest two channel acquisition, full FRAP acquisition and analysis workflow and online ratio imaging and analysis
- Save protocols for future use so that it’s easier to repeat regular experiments
- Customize the interface for simpler navigation
EnlargeLive cell imaging of Drosophila S2 cells showing the requirement for ISWI in anaphase.
Integrated acquisition to analysis – many biological questions one 3D solution
When using the Volocity® 3D Image Analysis software for acquisition, your images are acquired directly into the software. You can immediately benefit from the greater accuracy offered by 3D visualization and analysis. You can also easily present and export findings with a range of options including QuickTime, AVI or WMV movies.
Volocity software supports images from other confocal, widefield or high content screening systems in addition to those from Volocity Acquisition, and allows the same tools to be used for visualization, analysis, measurement and validation of all 3D images in your workflow. You can use Volocity Visualization to explore your z-stacks as fully interactive 3D renderings and then identify, analyze and track objects in your 3D data using Volocity Quantitation.
With Volocity Restoration, significantly improve the quality and resolution of your images from the UltraVIEW VoX system and other confocal microscopy, widefield and high content screening systems to reveal more detail for visualization and achieve more accurate quantitation.
FRAP and Ratio Acquisition
Volocity Acquisition can be used to acquire and analyze FRAP and ratiometric imaging experiments by adding the optional FRAP Plug-in and Ratio Plug-in to your system. Volocity Quantitation must be included in the Volocity configuration in order to perform analysis.
EnlargeFRAP experiments to evaluate nucleosomal stability of novel H3 variants. A small nuclear area was photobleached (box) and the recovery of the fluorescent signal was monitored. Depicted is a short-term FRAP series (selected time points are shown) of GFP tagged H3 variants compared with GFP alone. Scale bar 5 μm.
The FRAP Plug-in supports the PhotoKinesis™ Accessory, which is an option for the UltraVIEW VoX system. This configuration has all the tools you need to acquire and analyze FRAP images, providing both raw and graphical data. In addition to FRAP experiments, the unit can be used for techniques such as FLIP, photoswitching (photoconversion) and acceptor photobleaching.
The Ratio Plug-in provides the ability to acquire 2D and 3D ratiometric image pairs, with simultaneous online analysis, or post-acquisition analysis and charting of results.