Image based High Content Analysis can support all signaling pathway assays which rely on the translocation of a fluorescently labeled signaling molecule. Transcription factor activation, for example, can be monitored based on their translocation from cytosol to nucleus. Many other signaling molecules translocate from the cytosol to receptor complexes at the plasma membrane.
Akt3-GFP in untreated cells (left) and in activated cells (right).
Our solutions for Signaling Pathway Analysis
Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical signalling pathway assays and unlimited possibilities for customization:
Opera High Content Screening System
- High sample throughput with multi-color parallel image acquisition and over 100,000 data points per day
Operetta Compact High Content Screening System
- High speed with large field of view and large cell number captured with every image
- Ready-made image analysis sequences for transcription factor activation (cytosol to nucleus translocation), signal molecule recruitment to the plasma membrane, membrane ruffling (quantification of marker in membrane) and documentation on how to customize
- Unlimited possibilities for adding parameters and modifying readout
Acapella Image Analysis Software
- Image analysis sequences for cytosol to nucleus translocation and signal molecule recruitment to the plasma membrane cytosol, tools for quantification of markers in membrane and cytoplasmic regions
- Unlimited possibilities for adding parameters and modifying readout
Columbus High Volume Data Management and Analysis System
- Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data
cell::explorer Automated High Content Screening System
- Full environmental control and workstation with hand-over of plates from imager to incubator for short-term and long-term live cell assays
You may also be interested in: Live Cell Imaging.