Protein expression can be very easily quantified in High Content Analysis using either fluorescent protein tags or fluorescently labeled antibodies. Expression on the plasma membrane, in the cytosol and in the nucleus can be distinguished by defining the respective cellular regions.
Example for membrane bound marker (left), blue – Hoechst 33342 nuclear dye, green – membrane bound receptor labeled by a fluorescent protein. Region of Interest for protein detection (right).
Our Protein Expression solutions
Drawing on many years of experience and in-depth knowledge, PerkinElmer offers solutions for every throughput, a starting point for typical protein expression assays and unlimited possibilities for customization:
Opera High Content Screening System
- High sample throughput with multi-color parallel image acquisition and over 100,000 data points per day
Operetta Compact High Content Screening System
- High speed with large field of view and large cell number captured with every image
- Ready-made image analysis sequences for protein expression in cytosol, on plasma membrane and in nucleus, including documentation on how to customize
- Unlimited possibilities for adding parameters and modifying readout
Acapella Image Analysis Software
- Image analysis sequences for protein expression in cytosol, on plasma membrane and in nucleus
- Unlimited possibilities for adding parameters and modifying readout
Columbus High Volume Data Management and Analysis System
- Enterprise level data management from assay development to high throughput screening seamlessly linking high content data, research data, in vivo imaging data, result management, screening statistics, biological and chemical data
cell::explorer Automated High Content Screening System
- Full environmental control and workstation with hand-over of plates from imager to incubator for short-term and long-term live cell assays
- Scalable and fully automated workstation including compound handling, reagent addition, incubation, cell fixation and staining
- Instruments with readout other than imaging can be included, such as whole well fluorescence / luminescence (e.g. EnVision, EnSpire)
You may also be interested in: Live Cell Imaging.