Automated PCR Setup using the JANUS Workstation
The JANUS® Automated Workstation can be configured to reliably setup sample plates for PCR amplification, where post-PCR products are used for a variety of downstream applications such as SNP detection, genotyping, DNA sequence analysis, RNAi screening, and microarray construction. The same workstation can also prepare PCR sample plates for Real-Time PCR analysis including quantitative PCR (QPCR), disease gene identification and multiplexed molecular diagnostic assays.
The JANUS automated liquid handling workstation can enable:
- Multiplexed genotyping sssays
- Taqman-based PCR genotyping assays
- Real-time PCR analysis
- SNP analysis
- Cycle sequencing reaction setup
- DNA sequence plate setup - for loading onto capillary electrophoresis (CE) sequencers
Get the benefits:
- Fully automated walk-away PCR setup for STR genotyping provides consistent, reliable downstream analysis
- Choose between Amp tubes or PCR plates
- Achieve more throughput with the 96-tip Modular Dispense Technology™ (MDT) arm
- Filter barrier tips minimize the risk of cross contamination during all PCR setup steps
- Varispan™ arm features Accusense™ with independent liquid level sensing for each tip for optimal precision and minimal sample carry-over
Recommended JANUS system configurations
PCR Setup
- JANUS Mini, Standard or Expanded Platform with Varispan (4- or 8-Tip) Arm
- VersaTip™ Plus Option
- Low Volume Disposable Tip
- Optional temperature control devices such as Inheco Thermoshake, Teleshake70, Automated 105/70C Quad Controller, Inheco Temperature Control device.
Combined DNA Isolation, PCR Setup & PCR Cleanup
- Same configuration as for DNA Isolation
- Low Volume Disposable Tip