Automated Normalization using the JANUS Workstation
Many genomic samples have variable DNA contents and require dilution to a constant amount of nucleic acid in order to successfully perform downstream analyses such as multiplexed PCR-based genotyping. The JANUS® Automated Workstation offers a complete genomics solution that includes a normalization and dilution procedure following DNA quantification. DNA concentrations can be measured using a fluorescent DNA stain or determination of the absorbance ratio 260/280 nm, or a luminescence assay.
After quantification, the JANUS DNA Normalization Protocol uses output DNA concentration data to ‘intelligently’ dilute the DNA to a desired target amount per sample well, usually 1 ng. Once the normalization protocol is completed, samples are ready for further analysis.
The JANUS automated liquid handling workstation can enable:
- Normalization and dilution using JANUS Normalization Protocol
- Normalization using JANUS Victor Normalization Option with Workout data analysis software package
- Normalization following fluorescent quantification using OliGreen® or PicoGreen® assay
- Normalization following luminescent quantification using human specific Promega AluQuant assay
Get the benefits:
- Fully automated genomic DNA quantification and normalization protocols provide diluted DNA suitable for reliable downstream multiplexed genotyping assays
- High throughput processing increases throughput ~10 fold compared to manual processing
- Disposable filter barrier tips minimize risk of cross contamination
- Uses same JANUS workstation configuration as for PCR setup
- A combined DNA Normalization & PCR Setup protocol carries out both DNA normalization/dilution and genotyping PCR setup
Recommended JANUS system configurations
Normalization
- JANUS Mini, Standard or Expanded Platform with Varispan (4- or 8-Tip) Arm
- VersaTip™ Plus
- Low Volume Disposable Tip
Combined DNA Isolation, Normalization & PCR Setup
- Same configuration as for DNA Isolation
- Low Volume Disposable Tip