Quantification and Normalization with JANUS Automated Workstations

The JANUS® Forensic Workstation with PCR Setup configuration offers a complete strategy for downstream forensic analysis that includes DNA quantification assay setup followed by normalization and dilution. These are needed for casework and many database sample types prior to STR PCR and CE plate setup assays. Casework samples have widely varying DNA content and require quantification and diluted to uniform concentrations. Reliable and accurate normalization is needed to produce quality multiplexed STR profiles and DNA sequence data. The preferred forensic quantification method currently is real time quantitative PCR (QPCR) using the ABI Quantifiler PCR Amplification Kit. The JANUS Forensic Workstation carries out fully automated QPCR setup including dilution of the kit standard.

The JANUS automated liquid handling workstation can enable:

  • Fully Automated Real Time PCR setup
  • Supports fluorescent quantification using OliGreen® or PicoGreen® setup
  • Normalization using JANUS Victor3 Normalization Option
  • Normalization and dilution using JANUS QPCR Normalization Protocol for Forensics & Genomics
  • Taqman-based QPCR using Human and Y-specific ABI Quantifiler PCR Amplification Kits
  • Taqman-based QPCR using CFS-HumRT QPCR Protocol

Get the benefits:

  • Fully automated forensic DNA quantification and normalization protocols provide diluted DNA suitable for reliable downstream STR genotyping
  • Robust software tools and report templates support your processes and analysis requirements.
  • Embedded contamination controls ensure precision pipetting on a per tip basis.
  • A variety of protocols including DNA normalization and dilution, as well as PCR setup can be automated on the same workstation.

Recommended JANUS system configurations

Quantification & Normalization

  • JANUS Mini, Standard or Expanded Platform with Varispan™ (4- or 8-Tip) Arm
  • VersaTip™ Plus
  • Low Volume Disposable Tip

Combined DNA Isolation & PCR Setup

  • Same configuration as for DNA Isolation
  • Low Volume Disposable Tip