PCR Setup for JANUS Automated Workstations

The JANUS® Automated Workstation provides a flexible platform to support a wide array of PCR setup applications, including real-time quantitative PCR (RT-QPCR) reactions and multiplexed small tandem repeat (STR) typing assays. JANUS can easily automate other common PCR protocols used in molecular diagnostic laboratories such as cycle sequencing, SNP and DNA sequencing analysis, as well as create customized PCR master mixes using DNA primers, buffers, enzyme and nucleotides.

The JANUS automated liquid handling workstation can enable:

  • Taqman-based PCR genotyping assays
  • Real Time PCR (RT-PCR) assays using ABI, Promega and other kits.
  • SNP Analysis Assays
  • Cycle Sequencing Reaction setup
  • DNA sequence plate setup

Get the benefits:

  • JANUS platform with both Varispan and Modular Dispense Technology® (MDT) arms provides flexibility for varying throughputs and assay setup requirements.
  • The 96-tip MDT arm provides highest throughput in 96- and 384-well PCR plate formats.
  • Varispan Low Volume Disposable Tip Option provides precise and accurate low-volume liquid handling.
  • High throughput processing increases throughput ~10 fold when compared to manual processing.
  • Embedded contamination controls ensure precision pipetting on a per tip basis.
  • A variety of protocols including DNA isolation, quantification, normalization and PCR setup can be automated on the same workstation.

Recommended JANUS system configurations:

PCR Setup

  • JANUS Mini, Standard or Expanded Platform with Varispan (4- or 8-Tip) Arm and/or MDT arm
  • VersaTip Plus
  • Low Volume Disposable Tip

Combined Nucleic Acid Isolation and PCR Setup

  • Same configuration as for Nucleic Acid Isolation
  • Low Volume Disposable Tip