PerkinElmer
  • Research Areas
  • Applied Genomics

Nucleic Acid Isolation and PCR Setup

We offer automated solutions for nucleic acid isolation and PCR setup to increase the throughput and reproducibility of PCR workflows for virtually any application. Our automated solutions for nucleic acid isolation, analysis, purification of PCR products, and PCR setup are designed to increase the efficiency of endpoint, real-time, RT, and multiplex PCR experiments.

PerkinElmer offers several different solutions for automated nucleic acid isolation based on your sample volume and throughput needs. These solutions are grounded in the proprietary chemagen bead-based nucleic acid isolation technology, delivering high-yield and purity DNA and RNA for PCR analysis. Additionally, we offer solutions for PCR product purification PCR products, which removing impurities that maintain PCR yield.

PerkinElmer offers automated microfluidic devices simplifying the analysis of input concentration and qualitative assessment of PCR products replacing the multiple, manual steps of slab gel electrophoresis with high levels of accuracy and throughput.

With liquid handlers ranging in size from benchtop to large integrated workstations, PerkinElmer offers PCR setup automation solutions to meet any of your throughput needs.

Explore the areas below to learn how you can increase the efficiency of your PCR experiments.

For research use only. Not for use in diagnostic procedures.

Solutions

Automated Nucleic Acid Isolation

With vast experience in research applications for Human Genetics, Biobanking, HLA Typing and Pathogen Detection, we have developed high-end solutions for nucleic acid isolation from multiple sample types, providing

  • Ready to use DNA/RNA in high yields and purity – suitable for sensitive research downstream applications like NGS, MLPA, chip based assays, Genotyping and many more
  • Up to 50 µg DNA/ml blood
  • High throughput, fast processing times – 1-96 samples/run, 10 µl – 10 ml sample vol. in 60 min.

The isolation of high-quality nucleic acids from various sample material is a key component of today’s molecular testing methods.

Our high-performance solutions consisting of chemagic instruments and kits provide an innovative and efficient answer for this challenge. chemagic kits enable the specific binding of DNA and RNA from a huge variety of different sample materials, like whole blood, serum/plasma and saliva, facilitating high yield and purity.

The separation of nucleic acid target molecules (genomic DNA, total RNA, pathogen DNA/RNA or even circulating cell-free DNA) is achieved through proprietary chemagen technology. chemagen technology captures nucleic acids by highly specific binding M-PVA (polyvinyl alcohol) magnetic beads that are thereafter attracted to metal rods, that have been magnetized by an electromagnet. While these magnetized rods transfer the nucleic acids bound to the particles through the different process solutions, the rod rotation switches on after deactivation of the electromagnet, which leads to efficient and homogeneous resuspension of the particles during the preparation steps. This results in cross-contamination-free, high-yield purity DNA/RNA and success in the following downstream applications.

For research use only. Not for use in diagnostic procedures.

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Automated PCR Setup

The automation of PCR setup increases throughput and reproducibility while greatly reducing hands-on time. Our portfolio of liquid handlers enables the rapid and precise setup of endpoint, real-time, RT, and multiplex PCR experiments. With platform sizes ranging from benchtop to large integrated workstations, any of your throughput needs can be addressed. We offer solutions for any of your PCR automation needs from simple PCR setup, to PCR setup with an integrated thermocycler, to fully automated solutions offering hands-free sample transfer, DNA and RNA isolation, and optional normalization.

  • Standardized PCR protocols improve reproducibility
  • Increased throughput and reduced hands-on time increases productivity
  • Convenient, easy-to-use software
  • Full traceability of reagents, samples, volumes and pipetting steps

On-Deck Thermocycler Integration

A dedicated on-deck thermocycler can be integrated into the automated liquid handler workstation for easy hands-free operation to suit your PCR automation needs.

Complete Automation of Nucleic Acid Isolation and PCR Setup

The chemagic Prime instrument is a fully automated solution offering hands-free sample transfer, DNA and RNA isolation, optional normalization, and PCR setup for research applications. This validated, single supplier solution offers high quality DNA and RNA which can be extracted from a variety of matrices.

For research use only. Not for use in diagnostic procedures.

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