PerkinElmer
  • Research Areas
  • Applied Genomics

Molecular Cytogenetics

Fast, precise, and cost-effective targeted molecular karyotyping

Molecular cytogenetics plays an important role in the characterization of chromosome anomalies and is a critical component of current genetic studies including research into both constitutional disorders and cancer.

PerkinElmer is bringing the newest molecular techniques to cytogenetic laboratories. Our innovative methodologies support:

  • Increased detection rates
  • Faster results at lower costs
  • Streamlined processes
  • Support from sample-to-result

Oligonucleotide-based microarrays and bead-based multiplex BACs-on-Beads® products have been specifically designed, developed and validated by cytogeneticists for cytogeneticists performing molecular karyotyping.

For research use only. Not for use in diagnostic procedures.

Applications

Molecular Karyotyping

Fast, precise, and cost-effective targeted molecular karyotyping

BACs are Bacterial Artificial Chromosomes, large cloned sequences of human DNA typically 170,000 bases long. BAC probes have long been used for FISH. The BACs-on-Beads® technology uses probes generated from selected BACs immobilized onto Luminex® xMAP® encoded beads. The resulting bead sets are used to assay chromosomal gains and losses from minute sample amounts with high throughput. BACs-on-Beads analysis comprises hybridization of DNA sample sequences to BAC-derived probes representing the identified target regions. Samples are enzymatically labeled with biotin, and after hybridization in a 96-well plate a fluorescent streptavidin-phycoerythrin reporter is bound to the biotin label. Male and female reference DNA is also hybridized and the fluorescence ratio between each sample and reference DNA are compared to provide a clear display of any copy number changes in the targeted regions. Sample labeling is done enzymatically, which provides significant amplification of samples down to 100-200 ng.

For research use only. Not for use in diagnostic procedures.

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Cytogenetic Microarrays

Cytogenetic microarrays offer a simple, reliable method for assessing chromosomal aberrations at a high resolution. PerkinElmer’s CGX microarrays detect chromosome aberrations and provide accurate, and comprehensive, genome-wide analysis in a rapid and cost effective manner.

For research use only. Not for use in diagnostic procedures. These products are not available in the USA.

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