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Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

  • Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
  • TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
  • Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
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Automation Compatible
  • Yes (232)
Experimental Type
  • In vitro (163)
Format
  • Microplates (177)
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"Enzymology" 1-50 of 288 Products and Services

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LabChip EZ Reader

EZ READER, SHIP LEVEL

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.

SureFire principle

AlphaScreen SureFire Phospho-p38 MAPK Kit

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1752.00 EUR - 18700.00 EUR
SureFire principle

AlphaScreen SureFire ATF1 (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-ATF1 (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-ATF1 (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1752.00 EUR - 18700.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total NFκB LANCE Ultra TR-FRET Detection Kit

LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
Price Range : 1330.00 EUR - 6000.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 29, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 29 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 759.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 519.00 EUR - 30100.00 EUR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1752.00 EUR - 18700.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 11, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 11 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 759.00 EUR
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 298.00 EUR - 2979.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2481) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (phosphorylated on Ser2481) in cellular lysates using Alpha Technology.
Price Range : 519.00 EUR - 30100.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-NFκB (Ser536) LANCE Ultra TR-FRET Detection Kit

LANCE Ultra phospho-NFκB (Ser536) kits are designed for the detection of phosphorylated NFκB in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1873.00 EUR - 6500.00 EUR
SureFire principle

AlphaScreen SureFire EIF4E (p-Ser209) Assay Kit

The AlphaScreen® SureFire® p-eIF4E (Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1752.00 EUR - 18700.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total ZAP-70 LANCE Ultra TR-FRET Detection Kit

LANCE Ultra total ZAP-70 kits are designed for the detection of ZAP-70 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-ZAP-70 assay.
Price Range : 1330.00 EUR - 6000.00 EUR

AlphaLISA Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 27 (H3K27ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1302.00 EUR - 23700.00 EUR
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1752.00 EUR - 18700.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Price Range : 519.00 EUR - 30100.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 519.00 EUR - 30100.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3195.00 EUR - 8000.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 32, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 32 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 621.00 EUR
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 6, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1635.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1 (Thr308) kits are designed for the detection of AKT1 (phosphorylated at T308) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
PerkinElmer

ProfilerPro Kinase Peptide Substrate 18, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 18 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 759.00 EUR
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-Src (Tyr419) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-Src (Tyr419) kits are designed for the detection of Src (phosphorylated at residue Y419) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1321.00 EUR - 4288.00 EUR

LANCE Ultra ULight™-PLK (Ser137) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRRSLLE
Price Range : 211.00 EUR - 768.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 519.00 EUR - 30100.00 EUR

Biotin-anti-H3K79me2 antibody

Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.
Price Range : 1302.00 EUR - 23700.00 EUR

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 1405.00 EUR - 6500.00 EUR
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR

AlphaLISA Anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 tri-methylated at lysine 27 (H3K27me3). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1302.00 EUR - 23700.00 EUR

AlphaLISA Anti-mono-methyl-Histone H4 Lysine 20 Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against Histone H4 that is mono-methylated at lysine 20 (H4K20me1). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1302.00 EUR - 23700.00 EUR
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1752.00 EUR - 18700.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 519.00 EUR - 30100.00 EUR
SureFire principle

AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit

The AlphaScreen® SureFire® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1736.00 EUR - 18800.00 EUR
PerkinElmer

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRQSLIE
Price Range : 282.00 EUR - 768.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H2A Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone 2A (H2A) in cell extracts or cells in culture medium.
Price Range : 792.00 EUR - 14800.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SLP-76 (Ser376) kits are designed for the detection of SLP76 (phosphorylated at S376) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 1020.00 EUR - 4417.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
AlphaLISA SureFire Ultra kits

Total ZAP-70 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ total ZAP-70 assay is a sandwich immunoassay for quantitative detection of total ZAP-70 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-ZAP-70.
Price Range : 519.00 EUR - 31300.00 EUR

Anti-methyl-Histone H3 Lysine 9 (H3K9me2) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 9 (H3K9me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1302.00 EUR - 23700.00 EUR

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