PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"Drug Discovery Screening Reagents" 1-50 of 1362 Products and Services

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AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH Assay Kit

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.
Price Range : 504.00 EUR - 29200.00 EUR

CCL2/MCP1 (mouse/rat) AlphaLISA Detection Kit

The AlphaLISA® Mouse/Rat C-C Motif Chemokine 2/Monocyte Chemoattractant Protein 1 (CCL2/MCP1) Detection Kit is designed for detection and quantitation of mouse or rat CCL2/MCP1 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Mouse CCL2/MCP1 can be quantitatively measured in mouse serum. The analyte in this kit is mouse CCL2/MCP1.
Price Range : 2997.00 EUR - 23400.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 504.00 EUR - 29200.00 EUR

LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3064.00 EUR - 7700.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 664.00 EUR - 23400.00 EUR
PerkinElmer

AlphaLISA SureFire Ultra Total Cofilin Lysate

Extra control lysates for the AlphaLISA® SureFire® Ultra total cofilin assay.
List Price : 22.00 EUR

LANCE Ultra ULight™-anti-GST

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.
Price Range : 414.00 EUR - 9800.00 EUR
AlphaLisa Analyte 2

GAD67 (Human) lyophilized (AlphaLISA)

Human GAD67 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA GAD67 Detection Kit, but can be ordered separately.
List Price : 306.00 EUR

IL-17A (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-17A Detection Kit is designed for detection and quantitation of human IL-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR
AlphaLisa Analyte 2

Leptin (mouse), lyophilized (AlphaLISA)

Mouse leptin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse leptin kit, but can be ordered separately.
List Price : 247.00 EUR
AlphaLisa Analyte 2

IFN-β (human), lyophilized (AlphaLISA)

Human Interferon β (IFNβ) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The standard corresponds to the IFN-ß 1a subtype . This standard is already provided in the AlphaLISA human IFNβ kit, but can be ordered separately.
List Price : 254.00 EUR
AlphaLISA SureFire Ultra kits

Total MEK1 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 504.00 EUR - 29200.00 EUR
PerkinElmer

LANCE Ultra Lysis Buffer 1 (5X)

LANCE Ultra Lysis Buffer 1 is used in select LANCE Ultra cellular phosphophorylation detection kits. This buffer is already provided in these kits, but can be ordered separately.
Price Range : 118.00 EUR - 607.00 EUR

HIV p24 AlphaLISA Biotin-Free Detection Kit

The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 715.00 EUR - 10300.00 EUR
PerkinElmer

AlphaLISA SureFire Ultra p-p38 MAPK (Thr180/Tyr182) Lysate

Extra positive and negative control lysates for the AlphaLISA® SureFire® Ultra phospho-p38 MAPK (Thr180/Tyr182) assay.
List Price : 22.00 EUR
Protein

IGF-1 (rat), lyophilized (LANCE)

Rat IGF-1 (insulin-like growth factor 1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra rat IGF-1 Detection Kit, but can also be ordered separately.
List Price : 304.00 EUR
AlphaPlex-545-antibody-bead2

Anti-Mouse IgG AlphaPlex-545 (Tb) Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 605.00 EUR - 24800.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT3 (Tyr705) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT3 (Tyr705) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaLISA immunoassay kits

IgG2a (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG2a antibodies in cell culture media and non-mouse serum.
Price Range : 715.00 EUR - 9900.00 EUR
LANCE Detection Kit

Fibrinogen (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Fibrinogen Detection Kit is designed for detection and quantitation of human fibrinogen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 795.00 EUR - 10600.00 EUR

ANGPTL4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 445.00 EUR - 9800.00 EUR

IL-17A (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 717.00 EUR - 20200.00 EUR

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3064.00 EUR - 7600.00 EUR
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total mTOR Assay Kit

The AlphaLISA® SureFire® Ultra Total mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 504.00 EUR - 29200.00 EUR

Residual Protein A LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR
AlphaLISA Buffer

AlphaLISA 5X Epigenetics Buffer 1

Pre-formulated buffer that can be used in histone H3 peptide-based epigenetic assays.
Price Range : 112.00 EUR - 606.00 EUR
AlphaLISA immunoassay kits

IL-1β (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 1β (IL-1β) enables the quantitative determination of porcine IL-1β in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1095.00 EUR - 10600.00 EUR
LANCE Detection Kit

IL-5 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-5 Detection Kit is designed for detection and quantitation of human interleuukin-5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR

ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR
AlphaLISA immunoassay kits

CXCL4/PF4 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
Price Range : 636.00 EUR - 19100.00 EUR
AlphaLISA immunoassay kits

Somatostatin (human) AlphaLISA Detection Kit

The AlphaLISA® Somatostatin Detection Kit is designed for detection and quantitation of somatostatin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 715.00 EUR - 10300.00 EUR
LANCE Detection Kit

TSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR
AlphaLISA Cellular Epigenetics kits

AlphaLISA Tri-Methyl-Histone H3 Lysine 27 (H3K27me3) Cellular Detection Kit

This kit is designed for the detection of tri-methylated Histone H3 Lysine 27 (H3K27me3) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 611.00 EUR - 10900.00 EUR
AlphaLISA immunoassay kits

Hyaluronic acid (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.
Price Range : 715.00 EUR - 10000.00 EUR
AlphaPlex-545-antibody-bead2

Anti-human IgG AlphaPlex 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 605.00 EUR - 24800.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-ERK1/2 (Thr202/Tyr204) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-ERK1/2 (Thr202/Tyr204) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaScreen Acceptor Beads

AlphaScreen Protein A Acceptor beads, 25 mg

AlphaScreen Acceptor beads conjugated to Protein A.
List Price : 8800.00 EUR
PerkinElmer

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1267.00 EUR - 4114.00 EUR

TSLP (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TSLP enables the quantitative determination of human TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 617.00 EUR - 14800.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SYK (Tyr525/526) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SYK (Tyr525/526) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaLISA immunoassay kits

IgG3 (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG3 antibodies in cell culture media and non-mouse serum.
Price Range : 715.00 EUR - 9900.00 EUR

Nidogen 1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Nidogen 1 Detection Kit is designed for detection and quantitation of Nidogen 1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR
LANCE Detection Kit

ANGPT1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPT1 Detection Kit is designed for detection and quantitation of human angiopoietin 1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 819.00 EUR - 10900.00 EUR

Leptin (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1519.00 EUR - 10100.00 EUR
Protein

Apo-CIII (human), lyophilized (LANCE)

Human Apo-CIII standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra ApoC3 Detection Kit, but can also be ordered separately.
List Price : 240.00 EUR
AlphaLISA immunoassay kits

IL-12 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-12 enables the quantitative determination of porcine interleukin-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 617.00 EUR - 18500.00 EUR

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3064.00 EUR - 7600.00 EUR
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 636.00 EUR - 19100.00 EUR

AlphaScreen Omnibeads

Single bead instrument control that contains the chemistry of both Alpha Donor beads and AlphaScreen Acceptor beads.
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IFN-γ Detection Kit

The AlphaPlex™ 545 (Terbium) human interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 474.00 EUR - 10300.00 EUR

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