PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1005 Products & Services

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AlphaLisa Analyte 2

IgM (human), lyophilized (AlphaLISA)

Human IgM standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IgM kit, but can be ordered separately.
List Price : 265.00 EUR
AlphaLISA assay principle

MANF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MANF enables the quantitative determination of mesencephalic astrocyte-derived neurotrophic factor (MANF) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 663.00 EUR - 19900.00 EUR

Chloramphenicol AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 459.00 EUR - 10800.00 EUR
AlphaLisa Analyte 2

IL-25 (human), liquid (AlphaLISA)

Human interleukin 25 (IL-25) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human IL-25 Detection Kit, but can also be ordered separately.
List Price : 317.00 EUR
Alpha_Donor_bead_1

Alpha Glutathione Donor beads

Alpha Donor beads coated with glutathione.
Price Range : 540.00 EUR - 10100.00 EUR
AlphaLISA immunoassay kits

IL-1α (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 692.00 EUR - 11000.00 EUR
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR
AlphaLISA immunoassay kits

Galectin-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human galectin-9 enables the quantitative determination of human galectin-9 (Gal-9) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 804.00 EUR - 15400.00 EUR

Osteoprotegerin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 663.00 EUR - 19900.00 EUR
AlphaLISA immunoassay kits

IL-1β (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 692.00 EUR - 11000.00 EUR
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 663.00 EUR - 19900.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 692.00 EUR - 24400.00 EUR
AlphaLISA immunoassay kits

CD28 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD28 enables the quantitative determination of human CD28 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 644.00 EUR - 15400.00 EUR

IL-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of human IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1631.00 EUR - 10800.00 EUR

CCL5/RANTES (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (CCL5/RANTES) Detection Kit is designed for detection and quantitation of mouse CCL5/RANTES in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3337.00 EUR - 24400.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Ser727) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
Price Range : 333.00 EUR - 20000.00 EUR
AlphaLISA immunoassay kits

IgG4 (human) Pharmacokinetic AlphaLISA Detection Kit

The AlphaLISA® Human IgG4 Pharmacokinetic Detection Kit is designed for the quantitative determination of human IgG4 in monkey serum and other samples, using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 1584.00 EUR - 10500.00 EUR

Anti-FITC AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to mouse monoclonal anti-FITC antibody. These beads can be used to capture FITC-tagged and fluorescein-labeled biomolecules for AlphaLISA no-wash assays.
Price Range : 630.00 EUR - 25900.00 EUR

IgM (human) AlphaLISA Detection Kit

The AlphaLISA® Human IgM Protein Detection Kit is designed for detection and quantitation of human IgM in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 815.00 EUR - 6800.00 EUR
AlphaLISA immunoassay kits

Hepatitis B Virus s Antigen (HBsAg) AlphaLISA Detection Kit

The AlphaLISA® immunoDetection Kit for Hepatitis B Virus Surface antigen (HBsAg) enables the quantitative determination of HBsAg in cell culture media and buffer using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 804.00 EUR - 15900.00 EUR
AlphaLISA immunoassay kits

IL-6 (bovine) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for quantitative detection of bovine IL-6. This kit permits detection of bovine IL-6 in bovine serum, plasma, and cell culture supernatants.
Price Range : 747.00 EUR - 11000.00 EUR
AlphaLISA immunoassay kits

FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 626.00 EUR - 11400.00 EUR
Alpha donor antibody coated

Anti-FLAG® Alpha Donor beads

Alpha Donor beads conjugated to anti-FLAG antibody. This bead can be used to capture FLAG-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.
Price Range : 516.00 EUR - 9400.00 EUR
AlphaLisa Analyte 2

IL-13 (bovine), lyophilized (AlphaLISA)

Bovine IL-13 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Bovine IL-13 Detection Kit, but can also be ordered separately.
List Price : 250.00 EUR
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IP10 Detection Kit

The AlphaPlex Samarium (Sm) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 738.00 EUR - 10800.00 EUR

Tissue Plasminogen Activator (human) AlphaLISA Detection Kit

The AlphaLISA® Human Tissue Plasminogen Activator (tPA) Detection Kit is designed for detection and quantitation of human tPA in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both tPA and pro-tPA.
Price Range : 1631.00 EUR - 10800.00 EUR
AlphaLISA immunoassay kits

Oncostatin M (human) AlphaLISA Detection Kit

The AlphaLISA® Human Oncostatin M Detection Kit is designed for detection and quantitation of oncostatin M in cell culture media, buffer solution, and serum using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 745.00 EUR - 10800.00 EUR
AlphaLISA immunoassay kits

IFN-β (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon β (IFNβ) Detection Kit is designed for detection and quantitation of human IFNβ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-ß 1a subtype.
Price Range : 1631.00 EUR - 10800.00 EUR
AlphaLisa Analyte 2

IL-17A (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-17 (IL-17A) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat IL-17A kit, but can be ordered separately.
List Price : 265.00 EUR
AlphaLISA immunoassay kits

CD80 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 804.00 EUR - 15400.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-MEK1 (Ser218/222) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-MEK1 (Ser218/222) in a multiplexed assay.
Price Range : 333.00 EUR - 20000.00 EUR

Streptavidin AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to streptavidin. These beads can be used to capture biotinylated proteins, peptides, nucleic acids (DNA and RNA), and small molecules for AlphaLISA no-wash assays.
Price Range : 630.00 EUR - 25900.00 EUR
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1582.00 EUR - 17100.00 EUR

C-peptide (human) AlphaLISA Kit

AlphaLISA® no-wash immunoassay kit for detection of human C-peptide (human connecting peptide) in serum, buffered solution or cell culture medium.
Price Range : 1584.00 EUR - 10500.00 EUR

AlphaScreen Histidine (Nickel Chelate) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 193.00 EUR - 14800.00 EUR

Anti-DIG AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a mouse monoclonal anti-Dig (digoxin) antibody. These beads can be used to capture Dig-labeled proteins, peptides, and other biomolecules to create AlphaLISA no-wash assays.
Price Range : 630.00 EUR - 25900.00 EUR
AlphaLisa Analyte 2

Hepatitis B Virus e Antigen (HBeAg), lyophilized (AlphaLISA)

Hepatitis B virus e antigen (HBeAg) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA HBeAg Detection Kit, but can also be ordered separately.
List Price : 433.00 EUR

S100A4/FSP1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human S100A4 enables the quantitative determination of human S100 calcium-binding protein A4 in serum, plasma, cerebrospinal fluid (CSF), buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 464.00 EUR - 10200.00 EUR
AlphaLisa Analyte 2

IL-1α (bovine), lyophilized (AlphaLISA)

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
List Price : 359.00 EUR
AlphaLisa Analyte 2

ApoA1 (human) Iyophilized (AlphaLISA)

Human Apo1A standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Apo1A Detection Kit, but can be ordered separately.
List Price : 326.00 EUR
Alpha Fc receptor bind

FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 746.00 EUR - 11400.00 EUR
AlphaLisa Analyte 2

Cry1Ab/1Ac, lyophilized (AlphaLISA)

Cry1Ab and Cry1Ac standards, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Cry1Ab/1Ac Detection Kit, but can be ordered separately.
List Price : 344.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-Acetyl CoA Carboxylase (Ser79) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-ACC (Ser79) assay is a sandwich immunoassay for quantitative detection of phospho-acetyl CoA carboxylase (phosphorylated on Ser79) in cellular lysates using Alpha Technology.
Price Range : 519.00 EUR - 30100.00 EUR
Protein

FGF23 (Human) lyophilized (AlphaLISA)

Human FGF23 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA FGF23 Detection Kit, but can be ordered separately.
List Price : 257.00 EUR
AlphaLISA immunoassay kits

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 464.00 EUR - 10200.00 EUR
AlphaLISA immunoassay kits

NGAL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
Price Range : 605.00 EUR - 11700.00 EUR
Alpha_Protein_Donor_bead

Protein A Alpha Donor beads

Alpha Donor beads conjugated to Protein A. This bead can be used to capture antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 516.00 EUR - 9400.00 EUR
AlphaLISA immunoassay kits

IFN-γ (ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ovine interferon gamma (IFNγ) enables the quantitative determination of ovine IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 464.00 EUR - 10200.00 EUR
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 IL-6 Detection Kit 

The AlphaPlex™ 545 (Terbium) IL-6 Detection Kit is designed for the quantitative determination of human IL6 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 651.00 EUR - 10800.00 EUR
AlphaLISA immunoassay kits

IL-21 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-21 enables the quantitative determination of human interleukin-12 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 644.00 EUR - 15400.00 EUR

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