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AlphaLISA Kits for Biomarkers and Cytokines

Eliminate ELISA limitations - convert to AlphaLISA!

Convert troublesome traditional ELISAs to a much superior alternative-PerkinElmer's highly sensitive, homogeneous AlphaLISA. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers and other analytes.

AngiogenesisEPOTNFαTNFα (mouse)VEGFA (mouse)VEGFB
VEGFCVEGFDVEGF
Biologics/
Bioprocess		Albumin (human)CHO-PE. coli HCPIgAIgE
IgGIgMNSO-PResidual Protein A
CancerAFPCA125Caspase-3 activeCXCL11/I-TACEPO
ERBB2/HER2HGFR/c-METIFN-βMMP1MMP2
MMP3MMP9MMP9 (mouse)β-NGFPSA
TNFαTFF3TIMP1
CardiovascularAlpha-2 macroglobulinCardiac Troponin ID-dimerEPOICAM-1
MyeloperoxidaseMyoglobinPAI-1PCSK9
PlasminogenRenin/ProrenintPA
Central Nervous SystemAmyloid β 1-15Amyloid β 1-40Amyloid β 1-40 (high specificity)Amyloid ß 1-40 (mouse/rat)Amyloid β 1-42
Amyloid β 1-42 (high specificity)Amyloid β 1-42 (mouse/rat)Amyloid β 1-xAmyloid β oligomerssAPPα (C-term specific)
sAPPβsAPPβ (high sensitivity)Tau
InflammationCXCL1/GRO-αCCL2/MCP1CCL2/MCP1 (mouse/rat)CCL3/MIP-1αCCL4/MIP-1β
CCL5 / RANTESCCL5 / RANTES (mouse)CRPCXCL9/MIGCXCL10/IP-10
G-CSFGM-CSFGM-CSF (mouse)IFN-αIFN-γ
IFN-γ (mouse)IL1αIL1βIL1β (mouse)IL2
IL2 (mouse)IL3IL4IL5IL6
IL6 (mouse)IL7IL7 (mouse)IL8IL10
IL10 (mouse)IL11IL1β (rat)IL12 (p70)IL13
IL15IL15 (mouse)IL17IL17A (mouse/rat)IL18
TNFαTNFα (mouse)
MetabolicAdiponectinAdiponectin (mouse)Albumin (human)Albumin (mouse)C-peptide (mouse/rat)
GLP-1Growth HormoneIGF1IGF2Insulin
LeptinProlactin
VirologyHIV p24 (high sensitivity)HIV p24

Many Benefits of Alpha Technology

You have several options to realize the benefits of AlphaLISA:

  • AlphaLISA kits for specific analytes
  • AlphaLISA Toolbox for constructing your own assays
  • OnPoint Reagent Services in which PerkinElmer develops, optimizes and transfers your custom assay for you

Learn more about OnPoint Reagent Services

References:

A Luminescent Oxygen Channeling Immunoassay for the Determination of Insulin in Human Plasma
Fritz Poulsen and Kirsten Borres Jensen, J Biomol Screen OnlineFirst, published on January 26, 2007

Conversion of ELISA to No Wash Technologies
James R. Beasley, Robert Swanson, Jingchun Yang, and David Dunn. Pharmacopeia Drug Discovery

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Easy automation for a broad range of analytes

AlphaLISA is preferable to ELISAs for a wide range of applications. Easy to automate and miniaturize, AlphaLISA assays are perfect for analytes requiring a high-throughput format or ultra-sensitivity and a wide dynamic range. The reliable no-wash technology saves your lab time and money.

AlphaLISA immunoassay kits

Each AlphaLISA immunoassay kit has five components:

  • AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
  • Streptavidin-coated Donor beads
  • Biotinylated anti-analyte antibody #2
  • Lyophilized analyte
  • AlphaLISA Immunoassay buffer (10x)

The kits can be used in a 96-, 384- or 1536-well format.


Quick Links

Resources

Products

AlphaLISA Adiponectin Immunoassay Research kit

Adiponectin is a hormone secreted by differentiated adipocytes that regulates energy homeostasis and...  View more

AlphaLISA Albumin kit

Therapeutic Area:  Biological and metabolic LDL (in Buffer) 104.8 pg/mL  View more

AlphaLISA Alpha-fetoprotein Research kit

AlphaLISA Amyloid Precursor Protein β, Secreted Research kit

AlphaLISA Amyloid ß 1-x kit

Therapeutic Area: Central nervous systemLDL (in Buffer) 4.5 pg/mL  View more

AlphaLISA Amyloid ß Oligomers kit

Therapeutic Area: Central nervous system  LDL (in Buffer) 1.3 pmol/L  View more

AlphaLISA Amyloid β 1-15, Immunoassay Research kit

AlphaLISA Amyloid β 1-40 (high specificity), Immunoassay Research kit

AlphaLISA Amyloid β 1-42 (high specificity), Immunoassay Research kit

AlphaLISA Beta-Nerve Growth Factor Research kit

AlphaLISA Buffers

AlphaLISA cardiac Troponin I kit

Therapeutic Area: Cardiovascular LDL (in Buffer) 4.7 pg/mL  View more

AlphaLISA Caspase-3 active kit

"Therapeutic Area: Cancer LDL (in Buffer) 126 pg/mL "  View more

AlphaLISA CCL5 / RANTES kit

"Therapeutic Area: Inflammation LDL (in Buffer) 0.8 pg/mL"  View more

AlphaLISA CHO-P Immunoassay Research kit

CHO (Chinese Hamster Ovary) cells are commonly utilized as an expression system for the production of...  View more

AlphaLISA C-Reactive Protein Research kit

AlphaLISA CXCL1 / GRO-α, Immunoassay Research kit

AlphaLISA CXCL9 / MIG, Immunoassay Research kit

AlphaLISA D-dimer kit

"Therapeutic Area: Cardiovascular LDL (in Buffer) 41.3 pg/mL"  View more

AlphaLISA G-CSF Immunoassay Research kit

Granulocyte Colony-stimulating Factor, G-CSF (also named Colony Stimulating Factor-3, CSF3), is a 20...  View more

AlphaLISA GLP-1 (7-36 amide) Immunoassay Research kit

The Glucagon-like Peptide-1 (GLP-1) is a 30-31 oligopeptide, generated from proglucagon, secreted by...  View more

AlphaLISA GM-CSF Immunoassay Research kit

Colony-stimulating Factors (CSFs) are proteins necessary for the survival, proliferation and differentiation...  View more

AlphaLISA Growth Hormone Immunoassay Research kit

Human Growth Hormone (GH, GHN or GH1) is the major regulator of postnatal growth, having somatogenic,...  View more

AlphaLISA HGFR / c-MET, Immunoassay Research kit

AlphaLISA HIV p24 (high sensitivity) kit

"Therapeutic Area: Virology LDL (in Buffer) \1.8 pg/mL "  View more

AlphaLISA human Amyloid β 1- 15, lyophilized (0.1 µg)

AlphaLISA human Amyloid β 1-40, lyophilized (0.1 µg)

AlphaLISA human Amyloid β 1-42, lyophilized (0,1 µg)

AlphaLISA human CXCL1 / GRO-α, lyophilized (0.1 µg)

AlphaLISA human CXCL9 / MIG, lyophilized (0.3 µg)

AlphaLISA human HGFR / c-MET, lyophilized (3 µg)

AlphaLISA human ICAM-1, lyophilized (1 µg)

AlphaLISA human IL15, lyophilized (0.1 µg)

AlphaLISA human IL15, lyophilized 0.1 µg

AlphaLISA human MMP3, lyophilized (0.3 µg)

AlphaLISA human TAU, lyophilized (0.3 µg)

AlphaLISA human α2-Macroglobulin

AlphaLISA human α2-Macroglobulin, lyophilized (3 µg)

AlphaLISA ICAM-1, Immunoassay Research kit

AlphaLISA IFN-α, Immunoassay Research kit

AlphaLISA IFN-γ Immunoassay Research kit

Interferons (IFNs) activity has been discovered due to their antiviral effects. In humans, there are...  View more

AlphaLISA IgE kit

Therapeutic Area: Biological LDL (in Buffer) 6.4 pg/mL  View more

AlphaLISA IL10 Immunoassay Research kit

Human Interleukin 10 (IL10) is a homodimer composed of two subunits of 18 kDa each. It is produced by...  View more

AlphaLISA IL11 kit

Therapeutic Area: Inflammation LDL (in Buffer) 8.6 pg/mL  View more

AlphaLISA IL15, Immunoassay Research kit

AlphaLisa IL17 Immunoassay Kit Research Kit

AlphaLISA IL1β Immunoassay Research kit

IL1α and IL1b are central players of the immune response, displaying roles in inflammation both at local...  View more

AlphaLISA IL2 Immunoassay Research kit

Interleukin 2 (IL2), formerly known as T-cell Growth Factor (TCGF), is a 15 kDa immunoregulatory lymphokine...  View more

AlphaLISA IL3 Immunoassay Research kit

AlphaLISA IL6 Immunoassay Research kit

Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on the immune system, but also...  View more

AlphaLISA IL8 Immunoassay Research kit

Interleukin 8 (IL8), a member of the ELR+ CXC chemokine family, is an 8.4 kDa polypeptide that forms...  View more

AlphaLISA Insulin-like Growth Factor 1 Research kit

AlphaLISA Insulin-like Growth Factor 2 Research kit

AlphaLISA Interleukin 12 (p70) Research kit

AlphaLISA Interleukin 13 Research kit

AlphaLISA Interleukin 18 Research kit

AlphaLISA Interleukin 1α Research kit

AlphaLISA Leptin Immunoassay Research kit

Leptin is a 16 kDa hormone secreted by differentiated adipocytes. It regulates energy homeostasis as...  View more

AlphaLISA Matrix Metalloproteinase-1 Research kit

AlphaLISA Matrix Metalloproteinase-9 Research kit

AlphaLISA MMP3, Immunoassay Research kit

AlphaLISA Monocyte Chemoattractant Protein-1 Research kit

AlphaLISA mouse / rat Amyloid β 1-40, Immunoassay Research kit

AlphaLISA mouse / rat Amyloid β 1-42, Immunoassay Research kit

AlphaLISA mouse / rat C-Peptide, Immunoassay Research kit

AlphaLISA mouse / rat IL17A, Immunoassay Research kit

AlphaLISA mouse Adiponectin kit

Therapeutic Area: Metabolism LDL (in Buffer) 3.1 pg/mL  View more

AlphaLISA mouse Albumin, Immunoassay Research kit

AlphaLISA mouse Albumin, lyophilized (3 µg)

AlphaLISA mouse CCL2 / MCP-1, lyophilized (0,3 µg)

AlphaLISA mouse CCL5 / RANTES kit

Therapeutic Area: Inflammation LDL (in Buffer) 2.6 pg/mL  View more

AlphaLISA mouse GM-CSF kit

Therapeutic Area: Inflammation LDL (in Buffer) 2.0 pg/mL  View more

AlphaLISA mouse IL15, Immunoassay Research kit

AlphaLISA mouse IL15, lyophilized (0,3 µg)

AlphaLISA mouse IL17A, lyophilized (0,1 µg)

AlphaLISA mouse IL2, Immunoassay Research kit

AlphaLISA mouse IL2, lyophilized (0,1 µg)

AlphaLISA mouse IL7, Immunoassay Research kit

AlphaLISA mouse IL7, lyophilized (0,3 µg)

AlphaLISA mouse MMP9 kit

Therapeutic Area: Cancer LDL (in Buffer) 19.9 pg/mL  View more

AlphaLISA mouse VEGFA kit

Therapeutic Area: Angiogenesis LDL (in Buffer) 1.5 pg/mL  View more

AlphaLISA Myeloperoxidase Research kit

AlphaLISA Myoglobin kit

"Therapeutic Area: Cardiovascular LDL (in Buffer) 1 pg/mL"  View more

AlphaLISA NS0-P Immunoassay Research kit

NSO murine hybridoma cells are commonly utilized as an expression system for the production of human...  View more

AlphaLISA PAI-1 kit

"Therapeutic Area: Cardiovascular LDL (in Buffer) 39 pg/mL"  View more

AlphaLISA Plasminogen Research kit

AlphaLISA Prolactin Immunoassay Research kit

Prolactin (PRL) is an endocrine factor primarily synthesized in the lactotrophs of the anterior pituitary...  View more

AlphaLISA Protein A, lyophilized (0,3 µg)

AlphaLISA PSA Immunoassay Research kit

Human Prostate-Specific Antigen (hPSA, KLK3) is a 33 kDa glycoprotein of the kallikrein family present...  View more

AlphaLISA rat 1 C-Peptide, lyophilized (0,1 µg)

AlphaLISA rat IL1ß kit

Therapeutic Area: Inflammation LDL (in Buffer) 6.2 pg/mL  View more

AlphaLISA Receptor Tyrosine-Protein Kinase ErbB-2 (HER2) Research kit

AlphaLISA Renin Research kit

AlphaLISA Residual Protein A, Immunoassay Research kit

AlphaLISA Residual Protein A, Immunoassay Research kit

AlphaLISA rodent Amyloid β 1-40, lyophilized (0,3 µg)

AlphaLISA rodent Amyloid β 1-42, lyophilized (0,3 µg)

AlphaLISA TAU, Immunoassay Research kit

AlphaLISA Tissue Plasminogen Activator Research kit

AlphaLISA universal buffer, 5X

PBS/BSA buffer, 5X, 10 mL  View more

AlphaLISA universal buffer, 5X

PBS/BSA buffer, 5X, 100 mL  View more

AlphaLISA Vascular Endothelial Growth Factor B Research kit

AlphaLISA Vascular Endothelial Growth Factor C Research kit

AlphaLISA Vascular Endothelial Growth Factor D Research kit

AlphaLISA α2-Macroglobulin, Immunoassay Research kit

AlphaLISA  mouse / rat CCL2 / MCP1, Immunoassay Research kit

AlphaLISA® human CCL2 / MCP1, lyophylized (0.3 µg)

AlphaLISA® human ERBB2 / HER2, lyophylized (1 µg)

AlphaLISA® human IFN-α, lyophylized (0.3 µg)

Lyophilized human Interferon-alpha (IFN-α). One vial (0.3 ug) contains an amount of human IFN-α sufficient...  View more

AlphaLISA® human IFN-β, lyophylized (0.3 µg)

AlphaLISA® human IL12 (p70), lyophylized (0.3 µg)

AlphaLISA® human IL13, lyophylized (0.1 µg)

AlphaLISA® human ß-NGF, lyophylized (1 µg)

AlphaLISA® Human TNF α Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Tumor Necrosis Factor Alpha is a multifunctional...  View more

AlphaLISA® C-C motif chemokine 3 (Macrophage inflammatory protein 1-a) Research kits

AlphaLISA® C-C motif chemokine 4 (Macrophage inflammatory protein 1-b) Research kits

AlphaLISA® C-X-C motif chemokine 10 (10 kDa IFN g-induced protein) Research kits

AlphaLISA® C-X-C motif chemokine 11 (Interferon inducible T-cell a chemoattractant) Research kits

Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family....  View more

AlphaLISA® E.coli proteins (Host cell proteins) Research kits

AlphaLISA® HIV p24 Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. HIV p24 is the 230aa phosphorylated protein...  View more

AlphaLISA® human AFP, lyophylized (0.3 µg)

AlphaLISA® Human Amyloid β 1-40 Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Amyloid peptides result from the cleavage...  View more

AlphaLISA® Human Amyloid β 1-42 Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Amyloid peptides result from the cleavage...  View more

AlphaLISA® human CRP, lyophylized (3 µg)

AlphaLISA® Human EPO Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Erythropoietin hormone is a 34 kDa...  View more

AlphaLISA® human IgA, lyophylized (10 µg)

AlphaLISA® human IGF1, lyophylized (1 µg)

AlphaLISA® human IGF2, lyophylized (1 µg)

AlphaLISA® Human IgG Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. The glycoprotein immunoglobulin G (IgG),...  View more

AlphaLISA® human IgM, lyophylized (3 µg)

AlphaLISA® human IL18, lyophylized (0.3 µg)

AlphaLISA® human IL1α, lyophylized (0.1 µg)

AlphaLISA® human IL4, lyophylized (0.3 µg)

AlphaLISA® human IL5, lyophylized (0.3 µg)

AlphaLISA® human IL7, lyophylized (0.3 µg)

AlphaLISA® Human Insulin Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Insulin is a key player in the control...  View more

AlphaLISA® human MMP1, lyophylized (0.3 µg)

AlphaLISA® human MMP2, lyophylized (0.3 µg)

AlphaLISA® human MMP9, lyophylized (0.3 µg)

AlphaLISA® human Myeloperoxidase, lyophylized (1 µg)

AlphaLISA® human PCSK9, lyophylized (0.3 µg)

AlphaLISA® human Plasminogen, lyophylized (10 µg)

AlphaLISA® human Renin, lyophylized (0.3 µg)

AlphaLISA® human TFF3, lyophylized (0.1 µg)

AlphaLISA® human TIMP1, lyophylized (0.1 µg)

AlphaLISA® Human VEGF Research Immunoassay Kit

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Vascular Endothelial Growth Factor...  View more

AlphaLISA® Immunoglobulin A Research kits

AlphaLISA® Immunoglobulin M Research kits

AlphaLISA® Interferon b Research kits

Interferons (IFNs) are a family of cytokines produced in response to the presence of pathogens such ...  View more

AlphaLISA® Interferon g (mouse) Research kits

Interferon-gamma (IFN-γ) is a cytokine belonging to the Type II class of interferons (IFN). IFN-γ is...  View more

AlphaLISA® Interleukin 10 (mouse) Research kits

AlphaLISA® Interleukin 1b (mouse) Research kits

AlphaLISA® Interleukin 4 Research kits

AlphaLISA® Interleukin 5 Research kits

AlphaLISA® Interleukin 6 (mouse) Research kits

AlphaLISA® Interleukin 7 Research kits

AlphaLISA® Matrix Metalloproteinase-2 Research kits

AlphaLISA® Metalloproteinase inhibitor 1 (Tissue inhibitor of metalloproteinases) Research kits

AlphaLISA® mouse IFN-γ, lyophylized (0.1 µg)

AlphaLISA® mouse IL10, lyophylized (0.3 µg)

AlphaLISA® mouse IL1β, lyophylized (0.1 µg)

AlphaLISA® mouse IL6, lyophylized (0.3 µg)

AlphaLISA® Mucin-16 (Ovarian carcinoma antigen CA125) Research kits

AlphaLISA® Proprotein convertase subtilisin/kexin 9 Research kits

AlphaLISA® sAPPa (C-term specific) Amyloid Precursor Protein a, Soluble Research kits

AlphaLISA® sAPPb (high sensitivity) Amyloid Precursor Protein β, Soluble Research kits

AlphaLISA® Trefoil factor 3 Research kits

AlphaLISA® Tumor Necrosis Factor a (mouse) Research kits

AlphaLISA® Tumor Necrosis Factor α (mouse) Research kits, 5,000 assay points