JoVE Online Video Presentations
Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed indexed journal devoted to the publication of biological, medical, chemical and physical research in a video format. The video-articles listed below use PerkinElmer in vivo imaging systems and/or imaging reagents. Video-articles are either open access, in which you can view the full video or a subscription is required to view the video in its entirity.
OpenAccessOpen Access Videos
Milica Momcilovic1, Sean T. Bailey2, Jason T. Lee3, Charles Zamilpa3, Anthony Jones3, Gihad Abdelhady1, James Mansfield4, Kevin P. Francis5, David B. Shackelford1 PRODUCTS: G8 PET/CT
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Elza van Deel1,2, Yanto Ridwan1, J. Nicole van Vliet1, Sasha Belenkov3, Jeroen Essers1,4,5 PRODUCTS: Quantum FX
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Nicholas M. Bernthal1, Brad N. Taylor2, Jeffrey A. Meganck2, Yu Wang3, Jonathan H. Shahbazian3, Jared A. Niska1, Kevin P. Francis2, Lloyd S. Miller3,4 Combined optical and μCT imaging in a mouse model of orthopaedic implant infection, utilizing a bioluminescent engineered strain of Staphylococcus aureus, provided the capability to noninvasively and longitudinally monitor the dynamics of the bacterial infection, as well as the corresponding inflammatory response and anatomical changes in the bone. PRODUCTS: IVIS Spectrum, Quantum FX, Living Image software, Xen29 bioluminescent Staphylococcus aureus strain
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Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue Xueli Zhang1,2, Chaincy Kuo3, Anna Moore1, Chongzhao Ran1 In this video report, we show the application of Cerenkov Luminescence Imaging (CLI) for interscapular brown adipose tissue in mice under activated and depressed conditions. PRODUCTS: IVIS Spectrum
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4D Multimodality Imaging of Citrobacter rodentium Infections in Mice James William Collins1, Jeffrey A Meganck2, Chaincy Kuo2, Kevin P Francis2, Gad Frankel1 Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection. PRODUCTS: IVIS SpectrumCT, XGI-8 gas anesthesia system, Living Image 4.3.1 software
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In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus Allison Poussard*, Michael Patterson*, Katherine Taylor, Alexey Seregin, Jeanon Smith, Jennifer Smith, Milagros Salazar, Slobodan Paessler Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over multiple time points ensures reduced animal requirements, costs, and overall morbidity to the animals utilized ensuring a more humane and more scientific means of disease study. PRODUCTS: IVIS Spectrum, XGI-8 gas anesthesia system, Living Image 4.0 software
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Madonna M. McManus, Raymond J. Grill A longitudinal examination of bone loss in the femurs and tibiae of adult mice was performed following spinal cord injury using sequential low-dose X-ray scans. Tibia bone loss was detected throughout the study, while bone loss in the femur was not detected until 40 days post injury. PRODUCTS: IVIS Lumina XR
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Peter Bannas*1, Alexander Lenz*1,2, Valentin Kunick1,2, William Fumey1,2, Björn Rissiek2, Joanna Schmid1,2, Friedrich Haag2, Axel Leingärtner3, Martin Trepel4, Gerhard Adam1, Friedrich Koch-Nolte2 This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies. PRODUCTS: IVIS 100
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| PRODUCTS: IVIS Lumina
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| Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas PRODUCTS: IVIS Spectrum, Luciferin
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In vivo Dual Substrate Bioluminescent Imaging PRODUCTS: IVIS 200, RediJect Coelenterazine, D-Luciferin
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| Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems PRODUCTS: IVIS Lumina II
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| Ex Vivo Infection of Live Tissue with Oncolytic Viruses PRODUCTS: IVIS 200
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| PRODUCTS: IVIS Spectrum
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| Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging PRODUCTS: IVIS Spectrum, Quantum FX uCT, Light Producing Cells (Bioware)
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| In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer PRODUCTS: IVIS Spectrum, D-Ludiferin
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| PRODUCTS: IVIS Lumina
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| In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum PRODUCTS: IVIS Spectrum
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| PRODUCTS: IVIS Lumina
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| In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum PRODUCTS: IVIS Spectrum
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| Use of Human Perivascular Stem Cells for Bone Regeneration PRODUCTS: IVIS Lumina II, Living Image
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| PRODUCTS: IVIS Imaging System, D-Luciferin
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A JoVE Subscription is Required To View These Videos in Their Entirity
Lukasz J. Ochyl1,2, James J Moon1,2,3 We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination. PRODUCTS: IVIS Series
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Malek Saleh1, Mohammed R. Abdullah1, Christian Schulz1, Thomas Kohler1, Thomas Pribyl1, Inga Jensch1, Sven Hammerschmidt1 Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria. PRODUCTS: IVIS Spectrum, Living Image 4.1 Software, XGI-8 Gas Anesthesia System
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In vivo Imaging Method to Distinguish Acute and Chronic Inflammation Jen-Chieh Tseng1, Andrew L. Kung2 We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages. PRODUCTS: IVIS Spectrum, Living Image 4.2 Software
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Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring Yingding Xu, Hongguang Liu, Edwin Chang, Han Jiang, Zhen Cheng Use of Cerenkov Luminescence Imaging (CLI) for monitoring preclinical cancer treatment is described here. This method takes advantage of Cerenkov Radiation (CR) and optical imaging (OI) to visualize radiolabeled probes and thus provides an alternative to PET in preclinical therapeutic monitoring and drug screening. PRODUCTS: IVIS Spectrum
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| Bioluminescent Bacterial Imaging In Vivo PRODUCTS: IVIS
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| PRODUCTS: IVIS Lumina
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| PRODUCTS: IVIS Imaging System
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| PRODUCTS: IVIS 200
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| PRODUCTS: IVIS 200
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| PRODUCTS: D-Luciferin
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| An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment PRODUCTS: IVIS Spectrum, Quantum FX uCT, Light Producing Cells (Bioware)
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| A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo PRODUCTS: IVIS Spectrum, D-Luciferin
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| PRODUCTS: IVIS Spectrum
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| Using Luciferase to Image Bacterial Infections in Mice PRODUCTS: IVIS Imaging System
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| PRODUCTS: IVIS Lumina
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| In vivo Imaging of Transgenic Leishmania Parasites in a Live Host PRODUCTS: IVIS 200, D-Luciferin
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| Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure PRODUCTS: IVIS 200, D-Luciferin
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| Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia PRODUCTS: IVIS 200
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| In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells PRODUCTS: IVIS Imaging System
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| DNA Vector-based RNA Interference to Study Gene Function in Cancer PRODUCTS: IVIS Imaging System
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| Murine Bioluminescent Hepatic Tumour Model PRODUCTS: IVIS Imaging System
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| Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery PRODUCTS: IVIS Imaging System
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