For Research Use Only. Not for Use in Diagnostic Procedures.
Undesired toxic side effects are still amongst the most common reasons for failure of new chemical entities during late stage drug development and can even lead to costly withdrawals of approved drugs from the market. It is therefore necessary to assess compounds in the early phases of the discovery process reliably for potential geno-, hepato-, nephro and cardiotoxic liabilities in order to reduce development costs and increase research efficiency. High content screening is a cost effective tool to run dedicated toxicity tests on relevant model systems.
Such dedicated assays include micronuclei assays to identify genotoxic effects, cell health studies on heptatocytes and kidney cells, advanced assays on 3D InSight™ Liver Microtissues, or using primary human cells, hypertrophy studies on cardiomyocytes.
But even functional drug discovery HCS assays can provide important information on potential unwanted properties of a compound in addition to the primary effect readout.
Publications using PerkinElmer High-Content Screening Products
- Schang G, Robaire B and Hales BF. Organophosphate Flame Retardants act as Endocrine-Disrupting Chemicals in MA-10 Mouse Tumor Leydig Cells. Toxicological Sciences. 2016; 150(2):499-509.
- Willy JA, Schulte NE, Kreklau EL, Walgren JL, Renninger ML and Baker TK. In Vitro L6 Irritation Assay Predicts Clinical Injection Site Reactions for Small Molecules.Toxicological Sciences. 2016; 151(2):302-11.
- Kiris E, Burnett JC, Nuss JE, Wanner LM, Peyser BD, Du HT, Gomba GY, Kota KP, Panchal RG and Gussio R. Src Family Kinase Inhibitors Antagonize the Toxicity of Multiple Serotypes of Botulinum Neurotoxin in Human Embryonic Stem Cell-Derived Motor Neurons. Neurotoxicity Research. 2015; 27(4):384-398.
- Ivask A, Voelcker NH, Seabrook SA, Hor M, Kirby JK, Fenech M, Davis TP and Ke PC. DNA Melting and Genotoxicity Induced by Silver Nanoparticles and Graphene. Chemical Research in Toxicology. 2015; 28(5):1023-35.
- Ku S-K, Baek M-C and Bae J-S. Anti-inflammatory effects of methylthiouracil in vitro and in vivo.Toxicology and Applied Pharmacology. 2015; 288(3):374-86.
- Wang M, Liu C-X, Dong R-R, He S, Liu T-T, Zhao T-C, Wang Z-L, Shen X-Y, Zhang B-L and Gao X-M. Safety Evaluation of Chinese Medicine Injections with a Cell ImagingBased Multiparametric Assay Revealed a Critical Involvement of Mitochondrial Function in Hepatotoxicity. Evidence-Based Complementary and Alternative Medicine. 2015:379586.
- Bernardi M, Adami V, Albiero E, Madeo D, Rodeghiero F and Astori G. Absence of micronucleus formation in CHO-K1 cells cultivated in platelet lysate enriched medium. Experimental and Toxicologic Pathology. 2014; 66(2-3):111-116.
- Billis P, Will Y and Nadanaciva S. High‐Content Imaging Assays for Identifying Compounds that Generate Superoxide and Impair Mitochondrial Membrane Potential in Adherent Eukaryotic Cells.Current Protocols in Toxicology. 2014; 59:25.1.1-14.
- Ye Y, Weiwei J, Na L, Mei M, Kaifeng R and Zijian W. Application of the SOS/umu test and high‐content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles. Journal of Applied Toxicology. 2014; 34:1400-1408.
- van de Water F, Havinga J, Ravesloot W, Horbach G and Schoonen W. High content screening analysis of phospholipidosis: validation of a 96-well assay with CHO-K1 and HepG2 cells for the prediction of in vivo based phospholipidosis. Toxicology in Vitro. 2011; 25(8):1870-1882.
- Bramsen JB, Laursen MB, Nielsen AF, Hansen TB, Bus C, Langkjær N, Babu BR, Højland T, Abramov M and Van Aerschot A. A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity. Nucleic Acids Research. 2009; 37(9):2867-2881.