For Research Use Only. Not for Use in Diagnostic Procedures.
Opera Phenix Application Notes
Cardiac hypertrophy, characterized by abnormal enlargement of the heart muscle, is a major health risk where new approaches to drug discovery are needed.
In this application note, we outline how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes.
The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties.
In this application note, we show how a single nuclear stain can enable phenotypic profiling and how phenotypic profiles can be used to distinguish up to seven different cell types, without further staining or phenotypic markers.
The Opera Phenix™ High-Content Screening System has been specifically developed to enable users to perform highly multi-parametric phenotypic screens effectively through combining state-of-the-art hardware to allow simultaneous acquisition of up to four fluorescent channels, with Harmony® High-Content Imaging and Analysis Software for convenient experimental set-up and analysis. Here, we outline methods of improving the speed of the Opera Phenix's technical performance through the use of the system’s Synchrony Optics and Water Immersion Lenses.
Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, including cancer. Therefore, inhibiting protein-protein interactions represents an emerging area in drug design.
Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. However, the artificial models currently used for studying efflux transporter function lack the functional complexity of the natural liver environment. This application note shows how a high content assay was established in a complex 3D organotypic in vitro liver model system.
One of the most important processes involved in maintaining homeostasis is autophagy, the process of degrading cellular components such as lipids, large protein complexes or even whole organelles via the lysosomal route and altered autophagy is found in various pathological conditions.
In this application note, we validate a phenotypic image and data analysis workflow provided by Harmony® High Content Imaging and Analysis and High Content Profiler™ software using an autophagy assay as an application example.