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PerkinElmer 2016 UK High-Content Screening User Group Meeting

The PerkinElmer High-Content Screening (HCS) User Group Meeting was held on 15 September 2016 at the Centre for Stem Cells and Regenerative Medicine, King’s College, London, UK. With over 50 attendees from across the UK, this event provided a unique opportunity for users to appreciate the broad diversity of HCS applications; gain insights into new workflow solutions; share their experience, discuss challenges and learn about technology updates.

Presentations from academic experts demonstrated how high-content analysis using PerkinElmer’s Operetta, Opera and Opera Phenix platforms is being used to accelerate innovative research across many different fields, with the ultimate goal of improving human health.

With the kind permission of the speakers, we’re delighted to share further details of their work, as presented in the user group meeting.

Davide Danovi

Collaborative Phenotyping: HipSci and the Stem Cell Hotel
Davide Danovi, Centre for Stem Cells and Regenerative Medicine, King’s College London

Human pluripotent stem cells provide a virtually unlimited reservoir of homogeneous cells and are highly potent, offering considerable potential in gene discovery, disease modelling and drug development. The human induced pluripotent stem cell initiative (HipSci) brings together partners in genomics, proteomics, cell biology and clinical genetics to create a national induced pluripotent stem cells (iPSC) resource...

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Emma Shanks

Combining shRNA Screening With 3D Multiparametric Image Analysis
Emma Shanks, CRUK Beatson Institute, Glasgow

The Beatson Institute Screening Facility combines HCS with multiparametric phenotypic image analysis to support translational cancer research. Functional genomics tools including small interfering RNA (siRNA), short hairpin RNA (shRNA) and clustered regularly interspaced short palindromic repeats (CRISPR)-targeting RNA (crRNA) are used in both pooled and arrayed approaches to support target identification and validation in multiple types of cancer...

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Colin Johnson

High-Content Imaging of Ciliogenesis and Cilia Function
Colin Johnson, Leeds Institute for Molecular Medicine, Leeds

Primary cilia are microtubule-based organelles that act as a universal cellular “antennae” in vertebrates to receive and integrate signals from the extracellular environment. They have essential roles in mechanosensation and photosensation, allowing us to hear, see, excrete and reproduce. Defects in cilia cause a range of developmental phenotypes grouped into conditions called ciliopathies, an ever-expanding group of genetic disorders...

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Vernalis Research

High-Content Approaches to Understanding Chk1 Inhibitor Pharmacology
Andy Massey, Vernalis Research, Cambridge

Checkpoint kinase 1 (Chk1) has important roles in DNA replication in the absence of DNA damage induced by cytotoxic chemotherapeutic agents, particularly in cancer cells, which demonstrate increased sensitivity to Chk1 inhibitors. At Vernalis Research, the Operetta system is being used for single-cell immunofluorescent and live-cell imaging, to further understand the mode of action through which the Chk1 inhibitor, V158411, as monotherapy induces DNA damage and cell death in cancer cells...

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James Smith

Imaging and Analysis of Stem Cells and Stem Cell-Derived Cardiomyocytes
Dr James Smith, University of Nottingham, Nottingham

In the Denning Laboratory within the Department of Stem Cell Biology, the PerkinElmer Operetta system is used in stem-cell analysis and for the investigation of early differentiation markers and fully differentiated populations. A collaborative project is currently ongoing to develop metrics and quality standards for the scale-up of human induced pluripotent stem cells (iPSCs), which involves staining for specific early differentiation markers at specific time points and quantification of these...

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Rachel Clare

High-Content Screening at Liverpool School of Tropical Medicine: An Overview
Rachel Clare, Liverpool School of Tropical Medicine, Liverpool

The Anti-Wolbachia Consortium (A-WOL) is screening a diverse range of compounds to identify new macrofilaricidal drugs to treat onchocerciasis and lymphatic filariasis. A 384-well format assay has been developed, using the Operetta system in conjunction with optimised Wolbachia growth dynamics in the C6/36 Aedes albopictus mosquito cell line...

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For Research Use Only. Not for Use in Diagnostic Procedures.