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3D Microtissue Imaging

3D model systems

Organisms are three dimensional and the spatial organisation of cells in a tissue is essential for its proper function. Classical 2D cell cultures can therefore be insufficient model systems. In recent years 3D organotypic culture models such as the InSphero 3D Insight™ Microtissues and multicellular tumor spheroids have gained popularity as tools for research, drug discovery and toxicity studies. Several studies have shown the benefit of such models over 2D culture.

Due to their size 3D tissues pose characteristic challenges to any imaging system but benefit greatly from confocal imaging. As the sample is thick compared to the focal depth of the lens, spinning disk confocal systems perform better when the pinhole distance has been increased to minimize pinhole crosstalk, as it is the case for the Opera Phenix™ HCS system.

As 3D tissues are grown in aqueous media and the image quality is greatly improved when using water immersion lenses.

Related Application Notes

Application Note: Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix High-Content Screening System

Application Note: Quantitative Analysis of 3D Microtissue Growth and Biomarker Intensity using the Operetta High-Content Imaging System

Application Note: Cytotoxicity Studies on 3D Primary Liver Microtissues

Application Note: Quantitative Analysis of Embryoid Bodies Using the Operetta High-Content Imaging System

Application Note: 3D Analysis of Cell Invasion using the Operetta High-Content Imaging System

Publications using PerkinElmer High-Content Screening Products

For Research Use Only. Not for Use in Diagnostic Procedures.