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Bioluminescent Cell Lines


Overview


Our bioluminescent oncology cell lines (also known as Bioware® Brite cell lines) are stably transfected with the firefly luciferase reporter gene from Luciola Italica (Red-Fluc) that allows you to visualize the growth of the cells in vivo. Once the cell lines are injected into an appropriate mouse mode,subsequent expression of the optimized Red-Fluc luciferase gene allows you to to monitor early tumor development, monitor tumor growth and metastases even in deep tissue, quantify tumor burden in a whole animal model, and follow therapeutic responses non-invasively. PerkinElmer currently offers oncology cell lines covering a range of cancer models including breast, colorectal, prostate, lung, and lymphoma.

  • Bioware Brite cell lines contain the brighter, red-shifted Red- Fluc reporter gene and are bright enough to measure tumor growth in deep tissues, well before the tumors are palpable, allowing you to start collecting data at time points and tissue depths not possible with traditional micrometer measurements.
  • Bioware Brite Ultra Green are dual-labeled cell lines expressing Red-Fluc luciferase and GFP green fluorescent protein (allowing you to monitor tumor growth in vivo with luciferase and ex vivo (in analyses such as immunohistochemistry) with the fluorescent protein. PerkinElmer currently offers dual labeled cell lines in breast and prostate cancer models.
newfig1biolumASK.jpg 
Figure 1: 1 million HT1080-Red-FLuc cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse. Tumor growth was monitored for luciferase expression using the PerkinElmer IVIS® Spectrum at various time points. Mice were imaged 10 minutes post i.p. injection of luciferin at 150mg/kg at various time points. The image above shows tumor growth from a representative mouse.

All our cell lines meet the highest standards and are confirmed pathogen-free. The in vitro growth rates of these cell lines are similar to their parental lines and the stability of reporter expression is tested for a minimum of four weeks.

We also provide a wide range of bioluminescent pathogenic bacteria (both Gram-positive and Gram-negative) that can be used for drug discovery both in vitro and in vivo (including biofilm models) when used with our imaging systems. These light-producing microorganisms have been shown to work in a variety of acute and chronic infection models including pneumonia, meningitis, sepsis and biofilm infections.


Product selection table and culturing information


Blood cancer cell lines

Cell lineReporter type
TissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
K-562-Red-FlucFirefly luciferase (luminescence)Human: chronic myelogenous leukemia (CML)• Orthotopic tumor model (Intracerebral implantation)RPMI 1640 (ATCC Catalog No. 30-2001) with 10% Heat inactivated FBS
Puromycin at 2 µg/mLBW124735

Brain oncology

Cell lineReporter type
TissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
GL261-Red-FlucFirefly luciferase (luminescence)Mouse: GliomaMonitor mouse glioma xenograft non-invasively in real timeDMEM (ATCC cat#: 30-2002) with 10% FBS. Selection Marker: 100 ug/ml G418Puromycin at 2 µg/mLBW134246
U-87 MG-Red-FlucFirefly luciferase (luminescence)Human: glioblastoma; Brain• Subcutaneous tumor models
• Orthotopic tumor model (Intracerebral implantation)
Eagle's Minimum Essential Medium (ATCC Catalog No. 30-2003) with 10% Heat inactivated FBSPuromycin at 2 µg/mLBW124577

Breast cancer cell lines

Cell lineReporter type
TissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
MCF-7-Red-FlucFirefly luciferase (luminescence)Human: adenocarcinoma, mammary gland• Mammary fat pad orthotopic model
• Mammary fat pad with lung metastasis
Eagle’s MEM ATCC (Cat. No. 30-2003) with 10% Fetal Bovine Serum (FBS)Puromycin at 2 µg/mLBW119262
4T1-Red-FlucFirefly luciferase (luminescence)Mouse: mammary gland; adenocarcinoma• Mammary fat pad orthotopic tumor model
• Subcutaneous tumor models
RPMI 1640 ATCC Catalog Number 30-2001 with 10% heat inactivated FBSPuromycin at 5 µg/mLBW124087
4T1-Red-Fluc-GFPDual: Luciferase (luminescence) and GFP (fluorescence)Mouse: mammary gland; adenocarcinoma• Mammary fat pad orthotopic tumor models
• Subcutaneous tumor models
Ex vivo cell isolation using fluorescent activated cell sorter (FACS)
• Post genomic analyses of isolated tumor cells.
• Immunohistochemistry detection of GFP positive cells
RPMI 1640 ATCC (Catalog Number 30-2001) with 10% heat inactivated FBSThere is NO antibiotic selection for this cell line.BW128090

Colorectal oncology

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
Colo205-Red-FlucFirefly luciferase (luminescence)Human: adenocarcinoma; colorectal• Subcutaneous tumor models
• Subcutaneous tumor models with metastasis (SCID-bg mice)
RPMI 1640 (ATCC Catalog No. 30-2001) with 10% Heat inactivated FBSPuromycin at 2 µg/mLBW124317
HCT 116-Red-FlucFirefly luciferase (luminescence)Human: adenocarcinoma; colorectal• Subcutaneous tumor models
• Subcutaneous tumor models with metastasis (SCID-bg mice)
McCoy’s 5a Medium Modified, (ATCC catalog no. 30-2007) with 10% Heat inactivated FBSPuromycin at 2 µg/mLBW124318
HT-29-Red-FlucFirefly luciferase (luminescence)Human: adenocarcinoma; colorectal• Subcutaneous tumor models
• Subcutaneous tumor models with metastasis (SCID-bg mice) Note: Not recommended for colon orthotopic injection or implantation.
McCoy’s 5A, 1X (ATCC Catalog No. 10-050-CV) with 10% Heat inactivated FBSPuromycin at 2 µg/mLBW124353

Fibrosarcoma

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
HT1080-Red-FlucFirefly luciferase (luminescence)Human: Fibrosarcoma; adenocarcinoma• Testing immunomodulation of drugs in vivo
In vivo subcutaneous tumor models
Eagle’s MEM (ATCC Cat. No.30-2003) with 10% heat inactivated FBSPuromycin at 2 µg/mLBW128092

Liver oncology

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
HepG2-Red-FlucFirefly luciferase (luminescence)Human: Hepatocellular carcinomaIn vivo liver metastasis
• Subcutaneous models
Eagle’s MEM (ATCC Cat. No.30-2003) with 10% FBS. Selection Marker: 2 mg/ml PuromycinPuromycin at 2 µg/mLBW134280

Lung cancer

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
A549-Red-FlucFirefly luciferase (luminescence)Human: carcinoma, lung• Subcutaneous tumor models
• Experimental metastasis models (intravenous in SCID-bg mice)
RPMI-1640 (Mediatech Cat. No. 10-040-CV) + 10% FBSPuromycin at 2 µg/mLBW119266
LL/2-Red-FlucFirefly luciferase (luminescence)Mouse: carcinoma, lung• Subcutaneous tumor models
• Subcutaneous tumor models with metastasis (SCID-bg mice, successful at slower rate of growth for C57BL/6 and C57BL/6-albino mice)
• Experimental metastasis models (intravenous)
Note: Recommended mouse strains for in vivo imaging: SCID-beige (CR) and C57BL/6-albino (JAX).
Dulbecco's MEM (ATCC Cat. No. 30-2002) + 10% FBSPuromycin at 2 µg/mLBW119267
NCI-h560-Red-FlucFirefly luciferase (luminescence)Human: adenocarcinoma; Lung• Subcutaneous tumor models
• Subcutaneous tumor models with metastasis (SCID-bg mice)
• Orthotopic lung tumor models
RPMI 1640 ATCC Cat. No. 30-2001 with 10% heat- inactivated FBSPuromycin at 2 µg/mLBW124316

Melanoma

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number and tech sheet
B16-F10-Red-FlucFirefly luciferase (luminescence)Mouse: Melanoma• Subcutaneous tumor models
• Experimental metastasis models (intravenous)
RPMI 1640 (ATCC Catalog No. 30-2001) with 10% Heat inactivated FBSPuromycin at 2 µg/mLBW124734

Ovarian cancer

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
SKOV3-Red-FlucFirefly luciferase (luminescence)Human: adenocarcinoma, ovary• Subcutaneous tumor models
• Peritoneal dissemination models (intraperitoneal injection)
McCoy's 5a (ATCC Cat. No. 30-2007) + 10% FBSPuromycin at 2 µg/mLBW119276

Pancreatic cancer

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number
BxPc-3-Red-FlucFirefly luciferase (luminescence)Human: pancreas adenocarcinoma• Subcutaneous tumor models
• Orthotopic tumor model
RPMI 1640 (ATCC Catalog No. 30-2001) with 10% Heat inactivated FBSPuromycin at 2 µg/mLBW125058

Prostate cancer

Cell lineReporter typeTissueApplicationsCulture mediaAntibiotic selection – not required for culturingProduct number and tech sheet
LNCaP-Red-FlucFirefly luciferase (luminescence)Human: prostate; adenocarcinoma• Subcutaneous tumor models
• Subcutaneous tumor models with metastasis (SCID-bg mice)
• Orthotopic prostate tumor model
RPMI 1640 (ATCC Catalog No. 30-2001) with 10% UNHEATED FBSPuromycin at 2 µg/mLBW125055
PC-3-Red-FlucFirefly luciferase (luminescence)Human: Prostate Cancer• Experimental metastasis model
• Orthotopic prostate cancer models
Eagle’s MEM ATCC Cat. No. 30-2003 with 10% Heat inactivated FBS (Hyclone)Puromycin at 2 µg/mLBW128444
PC-3-Red-Fluc-GFPDual: Luciferase (luminescence) and GFP (fluorescence)Human: Prostate; adeno- carcinoma• Orthotopic and subcutaneous tumor models
Ex vivo cell isolation using fluorescent activated cell sorter (FACS)
• Post genomic analyses of isolated tumor cells
• Fluorescent detection of of GFP positive cells using Nuance Multispectral imaging systems
Eagle’s MEM ATCC Cat. No. 30-2003 with 10% Heat inactivated FBS (Hyclone)There is NO antibiotic selection for this cell line.BW133416

Lentivirus particles for creating your own bioluminescent cells

View our lentiviral particles page for products and information on creating your own bioluminescent cells.


Substrates


Please see our page on D-luciferin substrates.


Protocols



Citations


Browse or search our bioluminescent oncology cell line citations.