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Rediject 2-DG on the ASK


Overview


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Tumor cells exhibit a different metabolic pattern than normal cells, which includes an increase in glucose uptake (their main source of generating energy). Therefore, cancer cells exhibit an elevated glucose metabolism and up-regulation of glucose transporters (GLUTs) compared to non-tumor cells. XenoLight RediJect 2-DeoxyGlucosone (DG) 750 is a fluorescent probe for in vivo targeting of tumors which exhibit an elevated glucose uptake rate in comparison to surrounding tissues. To achieve maximum glucose targeting and enhanced tumor uptake, the probe has been designed to contain four 2-DG molecules per dye molecule.


Products and catalog numbers


ProductCatalog NumberEx/Em wavelength (nm)Validated ExperimentsApplicationsStorage and Stability
XenoLight RediJect 2-DG Probe760562 (20 doses) 

760561 (5 dose explorer kit)
750/780In vivo
Oncology
Inflammation
Arthritis
Technical Data Sheet

Using XenoLight RediJect 2-DG Probe in vivo


ProductRoute of InjectionMouse Dose (25 g)Tissue t 1/2Optimal imaging timeRoute of Metabolism/ background tissueIVIS settings
XenoLight RediJect 2-DG ProbeIV100 uL0.5 h3 hBladderIVIS 745/820

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Figure 1: Female C57 BL/6 albino mice were subcutaneously injected with 1 million LL2-luc cells. At 2-3 weeks, tumors were about 100-200 mm3. Mice were intravenously injected with 2-DG750 probe (10 nmol/mouse) and imaged with IVIS Spectrum at indicated time points using 745 nm excitation and 800 nm emission filter set. Quantification of the signal from tumor and control background area was plotted on the middle panel graph. Signal to background ratio was shown on the right panel.

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Figure 2: Co-Registered Image Showing Accumulation of 2-DG750 at MDA-MB231 Metastasis Sites

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Figure 3: Uptake of 2-DG750 by HCT116-luc2 Liver Metastasis. (left) Bioluminescent Signal From Liver Metastases. (right) Epi-Fluorescent Signal From 2-DG750 Uptake.

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Figure 4: Co-Registration of Bioluminescence (blue) and Fluorescence (magenta) Signals with μCT Image

As indicated above, RediJect 2-DG 750 probe showed specific accumulation to LL-2 tumors shortly after delivery. In addition, specific tumor labeling with the RediJect 2-DG 750 probe has also been demonstrated with Prostate (PC-3M) and Lung (NCI-H460) tumors, further validating the utility of this probe as a generic fluorescence reagent for in vivo tumor targeting.


Application notes and posters


  • Poster: Multivalent Fluorescent Probes for In Vivo Tumor Targeting


Tips


RediJect 2-DG control dye (Cat. No. 760567) is a non-reactive dye that can be used as a negative control when using the RediJect 2-DG-750 fluorescent imaging probe. This dye controls for passive dye accumulation, as any retention of probe that is not due to actual metabolism-related glucose uptake will also be observed with the control dye.


Citations


Please visit our Citations Library for references using XenoLight RediJect 2-DG Probe on the IVIS or on the FMT.