PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"Drug Discovery Screening Reagents" 1-50 of 1407 Products and Services

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AlphaScreen Omnibeads

Single bead instrument control that contains the chemistry of both Alpha Donor beads and AlphaScreen Acceptor beads.
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2481) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (phosphorylated on Ser2481) in cellular lysates using Alpha Technology.
Price Range : 508.00 EUR - 30600.00 EUR
AlphaLisa Analyte 2

IL-1β (porcine), lyophilized (AlphaLISA)

Porcine interleukin 1β (IL-1β) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IL-1β Detection Kit, but can also be ordered separately.
List Price : 732.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-BTK (Tyr223) LANCE Ultra TR-FRET Detection Kit

LANCE Ultra phospho-BTK (Tyr223) kits are designed for the detection of phosphorylated BTK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR
AlphaLISA immunoassay kits

IL-11 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 11 (IL-11) Detection Kit is designed for detection and quantitation of IL-11 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1578.00 EUR - 10500.00 EUR
AlphaPlex Samarium Acceptor Beads

Protein A AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
Price Range : 610.00 EUR - 25000.00 EUR
Fc-fragment-conjugated Alpha Donor beads

Fc-fragment (human) conjugated Alpha Donor Beads

Alpha Donor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
Price Range : 503.00 EUR - 9100.00 EUR
AlphaLisa Analyte 2

MMP9 (human), lyophilized (AlphaLISA)

Human Matrix Metalloproteinase-9 (MMP9) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human MMP9 kit, but can be ordered separately.
List Price : 256.00 EUR

AlphaScreen Histidine (Nickel Chelate) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 187.00 EUR - 14300.00 EUR
AlphaLisa Analyte 2

Troponin I (human), lyophilized (AlphaLISA)

Human Troponin I, lyophilized, for use as a standard for running standard curves in AlphaLISA no-wash immunoassays. This standard is already included in AlphaLISA Troponin kits, but can be ordered separately.
List Price : 549.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IKKα (Ser176/180) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IKKα (Ser176/180) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
PerkinElmer

Protein A AlphaPlex™ 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to Protein A, for use in Alpha no-wash multiplexing assays.
Price Range : 610.00 EUR - 25000.00 EUR

Apo-CIII (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Apo-CIII Detection Kit is designed for detection and quantitation of ApoC3 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 802.00 EUR - 10700.00 EUR
AlphaLisa Analyte 2

IgG3 (mouse), lyophilized (AlphaLISA)

Mouse IgG3 lyophilized standard, for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG3 isotyping kits, but can be ordered separately.
List Price : 247.00 EUR
AlphaLISA immunoassay kits

MMP-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Matrix Metalloproteinase-1 (MMP1) Detection Kit is designed for detection and quantitation of human MMP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP1 and pro-MMP1.
Price Range : 1578.00 EUR - 10500.00 EUR
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
AlphaLISA immunoassay kits

IFNβ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interferon β (IFNβ) enables the quantitative determination of porcine IFNβ in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1104.00 EUR - 10700.00 EUR
Alpha-Unconjugated-Donor-bead

AlphaScreen Unconjugated Donor Beads

Alpha Unconjugated Donor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own Alpha Donor beads for use in Alpha no-wash assays.
Price Range : 374.00 EUR - 10500.00 EUR
AlphaLisa Analyte 2

IL-11 (human), lyophilized (AlphaLISA)

Human Interleukin 11 (IL-11) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human IL-11 kit, but can be ordered separately.
List Price : 256.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IGF Receptor β (Tyr1135/1136) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IGF Receptor β (Tyr1135/1136) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaLISA immunoassay kits

CCL5/RANTES (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3023.00 EUR - 23600.00 EUR

DELFIA Eu-N1 ITC chelate & Eu standard, 1 mg

DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
List Price : 1880.00 EUR
AlphaLISA immunoassay kits

IL-12/IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.
Price Range : 723.00 EUR - 20300.00 EUR
PerkinElmer

IL-6 (human), lyophilized (LANCE)

Human IL-6 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human IL-6 Detection Kit, but can also be ordered separately.
List Price : 257.00 EUR
AlphaLisa Analyte 2

IgE (mouse), lyophilized (AlphaLISA)

Mouse IgE standard, for running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgE isotyping kit, but can be ordered separately.
List Price : 247.00 EUR

ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 826.00 EUR - 11000.00 EUR
AlphaLisa Analyte 2

CCL2 / MCP1 (human), lyophilized (AlphaLISA)

Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CCL2/MCP1 kit, but can be ordered separately.
List Price : 256.00 EUR

Anti-Chicken IgY AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-chicken IgY antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture chicken IgY antibodies for AlphaLISA no-wash assays.
Price Range : 610.00 EUR - 25000.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-HSP27 (Ser82) Assay Kit

The AlphaScreen® SureFire® p-HSP27 (Ser82) assay is a sandwich immunoassay for quantitative detection of phospho-HSP27 (phosphorylated on Ser82) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR
AlphaLISA immunoassay kits

IL-17A (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 723.00 EUR - 20300.00 EUR
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1549.00 EUR - 16700.00 EUR

AlphaScreen Mouse IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-mouse IgG conjugated AlphaScreen Acceptor beads.
Price Range : 187.00 EUR - 14300.00 EUR
AlphaLisa Analyte 2

IL-25 (mouse), liquid (AlphaLISA)

Mouse IL-25 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-25 Detection Kit, but can also be ordered separately.
List Price : 306.00 EUR

Fibronectin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human fibronectin enables the quantitative determination of human fibronectin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 778.00 EUR - 14900.00 EUR

Anti-DNP AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules.
Price Range : 826.00 EUR - 19300.00 EUR
AlphaLISA immunoassay kits

MCP2 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse MCP2 enables the quantitative determination of mouse monocyte chemoattractant protein 2 (MCP2; CCL8) in buffer and serum using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1211.00 EUR - 11500.00 EUR
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 508.00 EUR - 29400.00 EUR

Albumin (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection of human serum albumin (HSA) in urine, buffered solution, or cell culture medium.
Price Range : 3023.00 EUR - 23600.00 EUR
alphascreen surefire total

Total ERK (monoclonal) AlphaScreen SureFire Assay Kit

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1716.00 EUR - 18400.00 EUR
PerkinElmer

SureLight Allophycocyanin-anti-FLAG antibody, 1 mg, lyophilized

APC-labeled anti-FLAG antibody, for capturing FLAG-tagged proteins and peptides in LANCE TR-FRET assays.
List Price : 501.00 EUR
PerkinElmer

LANCE Ultra SMAD2 Positive Control Lysate

Control lysates for LANCE Ultra TR-FRET cellular phospho-SMAD2 detection assays.
List Price : 308.00 EUR
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-ERK1/2 (Thr202/Tyr204) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-ERK1/2 (Thr202/Tyr204) in a multiplexed assay.
Price Range : 323.00 EUR - 19500.00 EUR
AlphaLISA immunoassay kits

Amyloid ß 1-x (human) AlphaLISA Detection Kit

The AlphaLISA® Human Amyloid beta 1-x (Aß 1-x) Detection Kit is designed for detection and quantitation of human Aß 1-x in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3023.00 EUR - 23600.00 EUR

Osteoprotegerin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 641.00 EUR - 19200.00 EUR
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit

LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1330.00 EUR - 6000.00 EUR
Alpha donor antibody coated

Anti-HRP Alpha Donor Beads

Alpha Donor beads conjugated to anti-HRP antibody. These beads can be used to capture HRP-fusion proteins for Alpha no-wash detection, binding, and other assays.
Price Range : 485.00 EUR - 8800.00 EUR
AlphaLISA immunoassay kits

C-Reactive Protein (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1578.00 EUR - 10500.00 EUR
AlphaLISA immunoassay kits

PON-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 756.00 EUR - 14500.00 EUR
AlphaPlex-545-antibody-bead2

Anti-human IgG AlphaPlex 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 610.00 EUR - 25000.00 EUR
AlphaLISA immunoassay kits

B7-H5/VISTA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human V-domain Ig suppressor of T cell activation (VISTA) enables the quantitative determination of human VISTA (B7-H5, PD-1H) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 778.00 EUR - 14900.00 EUR

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