PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA immunoassay kits

TNFR1 (human, soluble) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for soluble human tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of soluble human TNFR1 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 6040.00 DKK - 85500.00 DKK
AlphaLisa Analyte 2

IgG3 (mouse), lyophilized (AlphaLISA)

Mouse IgG3 lyophilized standard, for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG3 isotyping kits, but can be ordered separately.
List Price : 1910.00 DKK
LANCE Detection Kit

IgG (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IgG Detection Kit is designed for detection and quantitation of Mouse IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 6420.00 DKK - 85500.00 DKK
AlphaLisa Analyte 2

TNFR1 (human), lyophilized (AlphaLISA)

Human tumor necrosis factor receptor 1 (TNFR1) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human TNFR1 detection kit, but can also be ordered separately.
List Price : 3250.00 DKK
AlphaLISA assay principle

IL-12 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-12 enables the quantitative determination of porcine interleukin-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 4840.00 DKK - 145100.00 DKK
AlphaLISA immunoassay kits

IgA (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 4990.00 DKK - 77900.00 DKK
AlphaLISA binding assay

HLA-DRA/LAG3 (human) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human HLA-DRA and LAG3, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to HLA-DRA and LAG3.
Price Range : 14100.00 DKK - 112700.00 DKK

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 1450.00 DKK - 111000.00 DKK
AlphaLisa Analyte 2

IL-6 (monkey), lyophilized (AlphaLISA)

Cynomolgus monkey IL-6 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA monkey IL-6 Detection Kit, but can also be ordered separately.
List Price : 2380.00 DKK
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
Price Range : 9910.00 DKK - 44400.00 DKK
AlphaLISA binding assay

PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.
Price Range : 14100.00 DKK - 112700.00 DKK
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 23500.00 DKK - 183600.00 DKK

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 23800.00 DKK - 59500.00 DKK
PerkinElmer

DELFIA® 1 nmol/L Terbium standard solution, 50 mL

For GLP or for general problem solving, lanthanide standard solutions are ready-to-use preparations of europium, samarium or terbium.
List Price : 840.00 DKK
AlphaLISA immunoassay kits

IL-17A (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 17A (IL-17A) enables the quantitative determination of porcine IL-17A in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 8580.00 DKK - 83000.00 DKK
PerkinElmer

AlphaScreen Biotinylated-GST

Biotinylated-GST, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA®, AlphaScreen®, or AlphaPlex™) no-wash assays.
List Price : 4060.00 DKK
AlphaLISA immunoassay kits

TIMP-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human tissue inhibitor of metalloproteinases-1 (TIMP1) enables the quantitative determination of human TIMP-1 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 6040.00 DKK - 85500.00 DKK

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.
Price Range : 4590.00 DKK - 188700.00 DKK

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.
Price Range : 5600.00 DKK - 81000.00 DKK
alphascreen surefire total

Total ERK (monoclonal) AlphaScreen SureFire Assay Kit

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 13300.00 DKK - 142600.00 DKK
AlphaLISA immunoassay kits

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 4980.00 DKK - 149400.00 DKK

Albumin (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Albumin Detection Kit is designed for the quantitative detection of mouse albumin in serum, urine, buffered solution or cell culture medium, using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 23500.00 DKK - 183600.00 DKK
LANCE Detection Kit

TSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 6420.00 DKK - 85600.00 DKK
PerkinElmer

LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)
Price Range : 14000.00 DKK - 48300.00 DKK
Protein

IgG (mouse), lyophilized (LANCE)

Mouse IgG standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Mouse IgG TR-FRET Detection Kit, but can be ordered separately.
List Price : 1930.00 DKK

Glucagon (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 6410.00 DKK - 85500.00 DKK
AlphaLISA immunoassay kits

IgG1 (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG1 antibodies in cell culture media and non-mouse serum.
Price Range : 5600.00 DKK - 77900.00 DKK
Protein

Cortisol, liquid (LANCE)

Cortisol standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Cortisol Detection Kit, but can also be ordered separately.
List Price : 6040.00 DKK

LANCE Ultra ULight™-anti-6xHis

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.
Price Range : 3070.00 DKK - 76300.00 DKK
AlphaLISA immunoassay kits

MMP-12 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MMP-12 enables the detection and quantitation of human matrix metalloproteinase-12 in serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP12 and pro-MMP12.
Price Range : 3590.00 DKK - 78500.00 DKK
AlphaLISA immunoassay kits

CXCL12/SDF-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CXCL12 (SDF-1) enables the quantitative determination of human CXCL12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 3490.00 DKK - 76400.00 DKK
AlphaLisa Analyte 2

IL-4 (mouse), lyophilized (AlphaLISA)

Mouse IL-4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-4 Detection Kit, but can also be ordered separately.
List Price : 2380.00 DKK
AlphaLISA immunoassay kits

TIMP-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human tissue inhibitor of metalloproteinases-2 (TIMP-2) enables the quantitative determination of human TIMP-2 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 6040.00 DKK - 85500.00 DKK
AlphaLISA immunoassay kits

IL-18BP (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-18BP enables the quantitative determination of human interleukin 18-binding protein in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 4550.00 DKK - 186900.00 DKK

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 9700.00 DKK - 176600.00 DKK
AlphaLISA immunoassay kits

IL-2 (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 5600.00 DKK - 81000.00 DKK

Fibronectin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human fibronectin enables the quantitative determination of human fibronectin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 6040.00 DKK - 116100.00 DKK

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 4840.00 DKK - 145100.00 DKK
LANCE Detection Kit

CXCL10a (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL10 Detection Kit is designed for detection and quantitation of human CXCL10 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 6410.00 DKK - 85500.00 DKK

IgG1 (bovine) AlphaLISA Detection Kit

The AlphaLISA® Bovine IgG1 Detection Kit is designed for detection, quantitation, and isotyping of bovine IgG1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 5410.00 DKK - 86400.00 DKK
AlphaLISA immunoassay kits

IL-28B (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-28B enables the detection and quantitation of human Interleukin-28B in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL28A, IL29 or Mouse IL28B. Cross reactivity with other species has not been tested.
Price Range : 3590.00 DKK - 78500.00 DKK
SureFire principle

Phospho-ERK (Thr202/Tyr204) Monoclonal AlphaScreen SureFire Assay kit

The AlphaScreen® SureFire® p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 13300.00 DKK - 142600.00 DKK
Protein

CCL4 (human), lyophilized (LANCE)

Human CCL4/MIP-1ß standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human CCL4/MIP-1ß TR-FRET Detection Kit, but can be ordered separately.
List Price : 1930.00 DKK

IL-11 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-11 Detection Kit is designed for detection and quantitation of human interleukin-11 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 6410.00 DKK - 85500.00 DKK
ATPlite luminescence assay kit

ATPlite Luminescence Assay System

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.
Price Range : 790.00 DKK - 16000.00 DKK

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLaISA® Human Factor VIII Biotin-Free Detection Kit is designed for the quantitative detection of human Factor VIII in buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 3700.00 DKK - 80900.00 DKK
AlphaLISA immunoassay kits

IgG2b (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG2b antibodies in cell culture media and non-mouse serum.
Price Range : 4990.00 DKK - 77900.00 DKK

IFN-γ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
Price Range : 5020.00 DKK - 163100.00 DKK

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 10500.00 DKK - 48200.00 DKK

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