PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

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Antibody conjugates
  • Anti-6X His (3)
  • Anti-acetyl-lysine (1)
  • Anti-c-Myc (4)
  • Anti-FITC (2)
  • Anti-FLAG (3)
  • Anti-GST (3)
  • Anti-H3K27ac (1)
  • Anti-H3K27me2 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K36me2 (1)
  • Anti-H3K4 (1)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K9/H3K27 (1)
  • Anti-H3K9ac (1)
  • Anti-H3K9me2 (1)
  • Anti-H4R3me (1)
  • Anti-HA (1)
  • Anti-human IgG (2)
  • Anti-methyl arginine (1)
  • Anti-mouse IgG (3)
  • Anti-O-linked-GlcNAc (1)
  • Anti-P-PKA (1)
  • Anti-P-Ser (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-P-Tyr-100 (1)
  • Anti-p53K382ac (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-Histone H3 (2)
  • Anti-phospho-I?Ba (1)
  • Anti-phospho-MEK1/2 (1)
  • Anti-phospho-p70S6K (1)
  • Anti-phospho-PKC (1)
  • Anti-phospho-PLK (1)
  • Anti-phospho-rpS6 (1)
  • Anti-phospho-threonine (1)
  • Anti-phospho-Topo IIa (1)
  • Anti-phospho-Tyrosine Hydroxylase (1)
  • Anti-rabbit IgG (3)
  • Anti-V5 (2)
  • Biotin (1)
  • Protein A (1)
  • Protein G (1)
  • PT66 (1)
  • PY20 (1)
  • Streptavidin (5)
Application
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (43)
  • Protein Analysis & Detection (15)
  • Protein-protein interactions (19)
Automation Compatible
  • Yes (90)
Detection Method
  • Time-Resolved Fluorescence (TRF) (83)
  • TR-FRET (83)
Fluorophore
  • Eu-W1024 (50)
  • Eu-W8044 (1)
  • SureLight APC (6)
  • SureLight-APC (1)
  • ULight (32)
Molecular modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (42)
Product Brand Name
  • LANCE (21)
  • LANCE SureLight (7)
  • LANCE Ultra (61)
Volume
  • 0-50 (1)
  • 51-100 (1)

"TR-FRET Toolbox Reagents" 1-50 of 90 Products and Services

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PerkinElmer

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 285.00 USD - 1164.00 USD

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8400.00 USD

ULight anti-FLAG

ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
Price Range : 422.00 USD - 10300.00 USD

LANCE Ultra ULight™-anti-6xHis

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.
Price Range : 428.00 USD - 10800.00 USD

LANCE Ultra ULight-TK peptide

ULight™-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
Price Range : 379.00 USD - 1119.00 USD

LANCE® Eu-W1024 Anti-Rabbit IgG

Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 1013.00 USD - 8900.00 USD

LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD
PerkinElmer

Eu-anti-V5 (LANCE TR-FRET)

Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 300.00 USD - 8400.00 USD

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 415.00 USD - 10500.00 USD

LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
Price Range : 381.00 USD - 838.00 USD

ULight anti-c-myc, 0.2 nmol

ULight™ labeled anti-c-myc antibody for LANCE® TR-FRET assays.
List Price : 415.00 USD
PerkinElmer

LANCE Eu-W1024 anti-FLAG

Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
Price Range : 309.00 USD - 8800.00 USD

LANCE Ultra ULight™-PKC substrate

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.
Price Range : 307.00 USD - 838.00 USD

LANCE Ultra Europium-anti-O-linked-GlcNAc Antibody

Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). For use in LANCE® Ultra post-translational modification assays.
Price Range : 3383.00 USD - 8400.00 USD

LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
Price Range : 1984.00 USD - 6800.00 USD

LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD

LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide

ULight™-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPK?1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR
Price Range : 380.00 USD - 1164.00 USD
PerkinElmer

LANCE Eu-W1024 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.
Price Range : 1013.00 USD - 8900.00 USD

LANCE® Eu-W1024 phospho-PKA sustrate antibody, 10 µg

Europium-labeled anti-phospho-PKA substrate antibody, for use in LANCE® TR-FRET kinase assays.
List Price : 2629.00 USD

ULight anti-human IgG

ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
Price Range : 415.00 USD - 10500.00 USD

ULight anti-c-myc, 20 nmol

ULight™ labeled anti-c-myc antibody for LANCE® TR-FRET assays.
List Price : 10500.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1399.00 USD - 4541.00 USD
PerkinElmer

SureLight Allophycocyanin-anti-Mouse antibody, 1 mg, lyophilized

APC-labeled anti-mouse IgG antibody, for capturing mouse IgG antibodies in LANCE TR-FRET assays.
List Price : 386.00 USD

LANCE Ultra ULight™-PLK (Ser137) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRRSLLE
Price Range : 224.00 USD - 813.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8400.00 USD

LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD
PerkinElmer

LANCE Eu-W1024 anti-FITC

Eu-W1024 labeled anti-FITC antibodies for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 309.00 USD - 8700.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 1984.00 USD - 6800.00 USD

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac)

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 9 (H3K9ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 1080.00 USD - 4677.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1984.00 USD - 6800.00 USD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 381.00 USD - 838.00 USD
PerkinElmer

LANCE Eu-W1024 Protein G

Europium-labeled Protein G, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 205.00 USD - 8900.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 205.00 USD - 9500.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG
Price Range : 1802.00 USD - 5800.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
Price Range : 229.00 USD - 13900.00 USD

LANCE Ultra ULight™-anti-GST

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.
Price Range : 428.00 USD - 10800.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 4 (H3K4me1-2)

Europium-labeled monoclonal antibody recognizing human H3 mono- and di-methylated at lysine 4 (H3K4me1-2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 403.00 USD - 10200.00 USD
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1013.00 USD - 8900.00 USD

LANCE® Eu-W1024 phospho-threonine antibody, 10 µg

Europium-labeled anti-phosphothreonine antibody, for use in LANCE® TR-FRET kinase assays. The antibody is an affinity purified rabbit polyclonal IgG (supplied by New England Biolabs).
List Price : 1206.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
Price Range : 1984.00 USD - 6800.00 USD
PerkinElmer

SureLight Allophycocyanin-anti-GST antibody, 1 mg, lyophilized

APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.
List Price : 482.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-Mouse IgG

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 1013.00 USD - 8900.00 USD

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
Price Range : 381.00 USD - 838.00 USD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 298.00 USD - 813.00 USD

ULight anti-rabbit IgG

ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
Price Range : 415.00 USD - 10500.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL
Price Range : 1984.00 USD - 6800.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)
Price Range : 1984.00 USD - 6900.00 USD
PerkinElmer

LANCE® Eu-W1024 phospho-serine antibody, 10 µg (Zymed)

Europium-labeled anti-phospho-serine antibody, for use in LANCE® TR-FRET kinase assays.
List Price : 1548.00 USD

"TR-FRET Toolbox Reagents" 1-50 of 90 Products and Services