TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

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Antibody conjugates
  • Anti-6X His (2)
  • Anti-acetyl-lysine (1)
  • Anti-c-Myc (1)
  • Anti-FITC (6)
  • Anti-FLAG (7)
  • Anti-GST (2)
  • Anti-H3K27ac (1)
  • Anti-H3K27me2 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K36me2 (1)
  • Anti-H3K4 (1)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K9/H3K27 (1)
  • Anti-H3K9ac (1)
  • Anti-H3K9me2 (1)
  • Anti-H4R3me (1)
  • Anti-HA (1)
  • Anti-human IgG (4)
  • Anti-methyl arginine (1)
  • Anti-mouse IgG (5)
  • Anti-O-linked-GlcNAc (1)
  • Anti-P-Akt (1)
  • Anti-P-PKA (1)
  • Anti-P-Ser (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-P-Ser 14-3-3 4E2 (1)
  • Anti-P-Ser PKC (1)
  • Anti-P-Ser/Thr-Phe (1)
  • Anti-P-Ser/Thr-Pro (1)
  • Anti-P-Tyr-100 (1)
  • Anti-p53K382ac (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-ATF-2 (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-Histone H3 (2)
  • Anti-phospho-I?Ba (1)
  • Anti-phospho-MEK1/2 (1)
  • Anti-phospho-mTOR (1)
  • Anti-phospho-p38a (1)
  • Anti-phospho-p70S6K (1)
  • Anti-phospho-PKC (1)
  • Anti-phospho-PLK (1)
  • Anti-phospho-rpS6 (1)
  • Anti-phospho-threonine (1)
  • Anti-phospho-Threonine-Proline (1)
  • Anti-phospho-Topo IIa (1)
  • Anti-phospho-Tyrosine Hydroxylase (1)
  • Anti-rabbit IgG (5)
  • Biotin (1)
  • Protein G (1)
  • PT66 (2)
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Application
  • Enzymatic (6)
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  • Protein Analysis & Detection (21)
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Automation Compatible
  • Yes (119)
Detection Method
  • Time-Resolved Fluorescence (TRF) (101)
  • TR-FRET (101)
Fluorophore
  • Eu-W1024 (64)
  • Eu-W8044 (1)
  • SureLight APC (6)
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  • ULight (50)
Molecular modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (62)
Product Family
  • LANCE (31)
  • LANCE SureLight (7)
  • LANCE Ultra (87)
Volume
  • 0-50 (33)
  • 51-100 (32)

"TR-FRET Toolbox Reagents" 1-50 of 122 Products and Services

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LANCE Ultra ULight™-MBP peptide, 0.125 nmole

MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.

List Price: 425.00 USD

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 3284.00 USD

LANCE Ultra ULight™-MBP peptide, 0.5 nmole

MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.

List Price: 298.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 199.00 USD

LANCE Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- and di-methylated at lysine 27 (H3K27me2-1). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 8200.00 USD

LANCE Ultra ULight™-anti-6xHis

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.

List Price: 10500.00 USD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG

List Price: 813.00 USD

LANCE Eu-W1024 anti-FLAG, 50 µg

Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.

List Price: 954.00 USD

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 8200.00 USD

LANCE Ultra ULight™-PKC substrate

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.

List Price: 813.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66), 50 µg

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 983.00 USD

ULight anti-FITC, 2 nmol

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.

List Price: 1420.00 USD

LANCE Ultra ULight™-poly GT

Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.

List Price: 813.00 USD

LANCE Ultra ULight™-MBP peptide, 5 nmoles

MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.

List Price: 813.00 USD

LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide, 0.5 nmole

ULight™-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR

List Price: 369.00 USD

LANCE® Eu-W1024 phospho-serine antibody, 10 µg (Zymed)

Europium-labeled anti-phospho-serine antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 1503.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2)

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 3284.00 USD

LANCE Eu-W8044 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays. The W8044 chelate provides more stability than the W1024 chelate.

List Price: 8600.00 USD

LANCE® Eu-W1024 Anti-Rabbit IgG

Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.

List Price: 8600.00 USD

LANCE Eu-W1024 Anti-HA

Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.

List Price: 13100.00 USD

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRQSLIE

List Price: 290.00 USD

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP

List Price: 277.00 USD

LANCE Eu-W1024 Anti-6xHis

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

List Price: 10500.00 USD

ULight anti-FLAG, 100 nmol

ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.

List Price: 10000.00 USD

LANCE Eu-W1024 Anti-phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 8100.00 USD

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR

List Price: 369.00 USD

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE

List Price: 370.00 USD

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK

List Price: 1750.00 USD

LANCE Eu-W1024 Anti-Mouse IgG

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.

List Price: 983.00 USD

LANCE Ultra ULight™-CREBtide

CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight™ dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.

List Price: 813.00 USD

LANCE Ultra Europium-anti-phospho-ATF-2 (Thr53) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human Activating Transcription Factor 2 (ATF-2; Swiss-Prot entry P15336) phosphorylated at Thr53. Thr53 of ATF-2 is phosphorylated by JNK and p38 kinases. Sequence motif recognized: VADQTPTPTRFLK

List Price: 1926.00 USD

LANCE® Eu-W1024 phospho-Akt substrate antibody, 10 µg

Europium-labeled anti-phospho-Akt substrate antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 4201.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 3284.00 USD

LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 8200.00 USD

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG

List Price: 6600.00 USD

LANCE® Eu-W1024 phospho-threonine-proline antibody, 10 µg

Europium-labeled anti-phospho-serine/threonine antibody, for use in LANCE® TR-FRET (TRF) kinase assays.

List Price: 1053.00 USD

LANCE Ultra ULight™-mTOR (Ser2448) Peptide, 5 nmoles

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser2448 of human mTOR. The ULight-mTOR peptide can be phosphorylated at Ser 2448 by the p70 S6 kinase and MST1 kinase. Peptide core motif : CAGAGRSRTRTDSYSAGQSV

List Price: 1131.00 USD

ULight anti-FLAG, 10 nmol

ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.

List Price: 1450.00 USD

LANCE Eu-W1024 anti-FITC, 1 mg

Eu-W1024 labeled anti-FITC antibodies for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.

List Price: 8400.00 USD

LANCE Ultra ULight™-Protein A

Protein A labeled with ULight™ dye.

List Price: 813.00 USD
ULight antibody

ULight anti-c-myc, 20 nmol

ULight™ labeled anti-c-myc antibody for LANCE® TR-FRET assays.

List Price: 10200.00 USD

LANCE® Eu-W1024 labeled anti-GST antibody

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.

List Price: 199.00 USD

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG

List Price: 6600.00 USD

SureLight Allophycocyanin-anti-GST antibody, 1 mg, lyophilized

APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.

List Price: 468.00 USD

ULight anti-human IgG, 2 nmol

ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.

List Price: 1420.00 USD

LANCE Ultra ULight™-mTOR (Ser2448) Peptide, 0.5 nmole

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser2448 of human mTOR. The ULight-mTOR peptide can be phosphorylated at Ser 2448 by the p70 S6 kinase and MST1 kinase. Peptide core motif : CAGAGRSRTRTDSYSAGQSV

List Price: 369.00 USD

LANCE Eu-W1024 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.

List Price: 8600.00 USD

LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

ULight™-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence : CAGAGAIETDKEYYTVKD

List Price: 369.00 USD

LANCE Ultra ULight-TK peptide

ULight™-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.

List Price: 368.00 USD

LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG

List Price: 6600.00 USD

"TR-FRET Toolbox Reagents" 1-50 of 122 Products and Services

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