PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

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Antibody conjugates
  • Anti-6X His (3)
  • Anti-acetyl-lysine (1)
  • Anti-c-Myc (2)
  • Anti-C-tag (2)
  • Anti-FITC (2)
  • Anti-FLAG (3)
  • Anti-GST (3)
  • Anti-H3K27ac (1)
  • Anti-H3K27me2 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K36me2 (1)
  • Anti-H3K4 (1)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K9/H3K27 (1)
  • Anti-H3K9ac (1)
  • Anti-H3K9me2 (1)
  • Anti-H4R3me (1)
  • Anti-HA (1)
  • Anti-human IgG (2)
  • Anti-methyl arginine (1)
  • Anti-mouse IgG (3)
  • Anti-O-linked-GlcNAc (1)
  • Anti-P-PKA (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-P-Tyr-100 (1)
  • Anti-p53K382ac (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-Histone H3 (2)
  • Anti-phospho-I?Ba (1)
  • Anti-phospho-MEK1/2 (1)
  • Anti-phospho-p70S6K (1)
  • Anti-phospho-PKC (1)
  • Anti-phospho-PLK (1)
  • Anti-phospho-rpS6 (1)
  • Anti-phospho-threonine (1)
  • Anti-phospho-Topo IIa (1)
  • Anti-phospho-Tyrosine Hydroxylase (1)
  • Anti-rabbit IgG (3)
  • Anti-V5 (2)
  • Biotin (1)
  • Protein A (1)
  • Protein G (1)
  • PT66 (1)
  • PY20 (1)
  • Streptavidin (5)
Application
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (42)
  • Protein Analysis & Detection (17)
  • Protein-protein interactions (21)
Automation Compatible
  • Yes (89)
Detection Method
  • Time-Resolved Fluorescence (TRF) (82)
  • TR-FRET (82)
Fluorophore
  • Eu-W1024 (50)
  • Eu-W8044 (1)
  • SureLight APC (6)
  • SureLight-APC (1)
  • ULight (31)
Molecular modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (41)
Product Brand Name
  • LANCE (21)
  • LANCE SureLight (7)
  • LANCE Ultra (63)

"TR-FRET Toolbox Reagents" 1-50 of 89 Products and Services

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PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
Price Range : 229.00 USD - 13900.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-Human IgG

Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 205.00 USD - 8900.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
Price Range : 1488.00 USD - 6800.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-6xHis

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
Price Range : 229.00 USD - 10800.00 USD

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 403.00 USD - 10200.00 USD

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 1488.00 USD - 6800.00 USD

LANCE Ultra ULight™-MBP peptide

MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
Price Range : 307.00 USD - 838.00 USD

LANCE Ultra ULight™-CREBtide

CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight™ dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 231.00 USD - 838.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342)

Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE
Price Range : 1984.00 USD - 6900.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 1984.00 USD - 6800.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 285.00 USD - 5800.00 USD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 298.00 USD - 813.00 USD

LANCE Ultra ULight™-PLK (Ser137) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRRSLLE
Price Range : 224.00 USD - 813.00 USD

LANCE® Eu-W1024 labeled anti-GST antibody

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
Price Range : 205.00 USD - 10100.00 USD

LANCE Ultra ULight™-anti-GST

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.
Price Range : 428.00 USD - 10800.00 USD

ULight anti-rabbit IgG

ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
Price Range : 415.00 USD - 10500.00 USD

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 381.00 USD - 838.00 USD
PerkinElmer
Price Range : 386.00 USD - 17900.00 USD
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1013.00 USD - 8900.00 USD

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD

LANCE® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.
List Price : 3300.00 USD
PerkinElmer

LANCE Eu-W1024 anti-FITC

Eu-W1024 labeled anti-FITC antibodies for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 309.00 USD - 8700.00 USD

LANCE Ultra ULight™-Protein A

Protein A labeled with ULight™ dye.
Price Range : 307.00 USD - 838.00 USD

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 415.00 USD - 10500.00 USD

ULight anti-human IgG

ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
Price Range : 415.00 USD - 10500.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 205.00 USD - 9500.00 USD

LANCE Ultra ULight™-IRS-1 (Tyr983) Peptide

ULight™-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence : CKKSRGDYMTMQIG
Price Range : 380.00 USD - 1164.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
Price Range : 1984.00 USD - 6800.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-Mouse IgG

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 1013.00 USD - 8900.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1013.00 USD - 8400.00 USD
PerkinElmer

SureLight Allophycocyanin-streptavidin (APC-SA), protein binding assay, Iyophilized

APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
Price Range : 386.00 USD - 10800.00 USD

LANCE Ultra ULight™-anti-6xHis

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.
Price Range : 428.00 USD - 10800.00 USD

LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide

ULight™-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPK?1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR
Price Range : 380.00 USD - 1164.00 USD

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 1080.00 USD - 4677.00 USD

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.
Price Range : 381.00 USD - 837.00 USD

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 9 and lysine 27 (H3K9/K27). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8400.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL
Price Range : 1984.00 USD - 6800.00 USD

LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
Price Range : 381.00 USD - 838.00 USD

LANCE Ultra ULight™-poly GAT

Poly Glu-Ala-Tyr (1:1:1) labeled with ULight™ dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 322.00 USD - 930.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 4 (H3K4me1-2)

Europium-labeled monoclonal antibody recognizing human H3 mono- and di-methylated at lysine 4 (H3K4me1-2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3383.00 USD - 8500.00 USD
PerkinElmer

LANCE Eu-W1024 anti-FLAG

Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
Price Range : 309.00 USD - 8800.00 USD
PerkinElmer

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR
Price Range : 285.00 USD - 1164.00 USD

LANCE Ultra ULight™-PKC substrate

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.
Price Range : 307.00 USD - 838.00 USD
PerkinElmer

SureLight Allophycocyanin-anti-6His antibody, 1 mg, lyophilized

APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.
List Price : 542.00 USD

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 285.00 USD - 1164.00 USD

LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

ULight™-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence : CAGAGAIETDKEYYTVKD
Price Range : 380.00 USD - 1164.00 USD

LANCE® Eu-W1024 Anti-Rabbit IgG

Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 1013.00 USD - 8900.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-HA

Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.
Price Range : 1013.00 USD - 13500.00 USD

"TR-FRET Toolbox Reagents" 1-50 of 89 Products and Services