PerkinElmer

Single Cell ICP-MS for Cancer Research

Metal-based chemotherapies such as Cisplatin are the most widely used class of drugs for the treatment of various cancers. Initially patients respond well to these drugs but often later relapse as the cancer displays resistance to the therapy. Metal-based chemotherapy works by forming crosslinks in DNA creating blockages which results in the cancer cells inability to replicate. Cellular uptake of metal-based drugs is related to tumor burden and consequently low intracellular levels of the drug are proportional to a decrease in tumor response.

Until now there has been little or no data about the distribution of the drug within individual tumor cells as traditional techniques can only measure the average concentration across the whole cell population. It’s clear that cells in the same population do not behave in the same way and the need to understand individual cell behavior is a trend across life science research in general. As a result, there is a growing need to analyze cellular uptake and distribution of metal-based or nanoparticle delivery of chemotherapy at the single cell level to determine if treatment is effective.

The innovative NexION® Single Cell ICP-MS system includes nebulizer technology allowing introduction of cells, one by one, as well as new software to calculate metal concentrations per cell. These will enable you to gain a better understanding of the mechanisms of how resistance develops in metal-based chemotherapies. With the NexION system you can rapidly detect and quantify metal concentrations in individual cancer cells to yield critical data about metal distribution in the cell population. This information may not only help you better understand chemotherapy resistance but can also lead to potential new therapies for cancer including in combination with other treatments such as immunotherapy.

Featured Products

Single Cell Analysis

Traditional methods for measuring uptake rates of metals in cells can be a time-consuming process with long instrument analysis and sample preparation time, often leading to qualitative or population-averaged results.

Nexion 2000 AwardThe award-winning NexION® ICP-MS Single Cell Analysis System, with its proprietary Asperon Single Cell Spray Chamber, Single Cell Micro DX Autosampler, and the Syngistix Single Cell Software Module, offers a unique opportunity to quantitatively measure the metal content in individual cells, unveiling new capabilities to study intrinsic metals and the uptake of dissolved (ionic) and nanoparticulate metals into cells, therefore providing new insights into drug delivery, toxicity assessment, bioavailability, and bioaccumulation mechanisms.

 

 

 

 

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NexION 2000 B ICP Mass Spectrometer

PerkinElmer’s NexION® 2000 is the most versatile ICP-MS on the market, featuring an array of unique technologies that combine to deliver the highest performance no matter what your analytical challenge. Discover the effortless versatility of an instrument that makes it easy to handle any sample matrix, any interference, and any particle size.

PerkinElmer received on April 11, 2018 the Application Award from LaborPraxis in the category Bio and Pharma Analysis for the NexION 2000 ICP-MS. We were awarded this prize during analytica 2018 in Munich, Germany, in acknowledgment of a new technique enabling users to quantify the amount of metal in an individual cell for the first time.

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NexION 1000 ICP Mass Spectrometer

The NexION® 1000 ICP-MS is the ideal high-throughput system for routine, multi-elemental, trace-level analyses that meet regulatory standards – and that works within your budget. It features a host of proprietary technologies that combine to deliver exceptional speed and operational simplicity, making your lab more efficient than ever before.

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Featured Content

New Research Evaluating Cisplatin Uptake in Ovarian Cancer Cells by Single Cell ICP-MS

Cisplatin, carboplatin, and oxaliplatin are the most widely used of platinum-based cancer chemotherapy drugs in the Western world. Cisplatin's effectiveness is due to its ability to bind to the DNA, resulting in DNA-platinum (Pt) adducts, which bend the DNA. The cells must then repair the DNA damage, otherwise DNA replication is blocked resulting in cell death. Many cancers are initially sensitive to platinum-based treatment, but patients frequently relapse with tumors displaying resistance to further cisplatin therapy.

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Quantification of Nanoparticle Uptake into Cancer Cells using Single Cell ICP-MS

Nanoparticles (NPs) have been of significant interest over the last two decades as they offer attractive benefits for drug delivery to overcome limitations in conventional chemotherapy.3 Nanoparticles can be engineered to carry both drugs and imaging probes to simultaneously detect and treat cancer. They may also be designed to specifically target diseased tissues and cells in the body. A number of nanoparticlebased cancer therapeutics have been approved for clinical use and/or are currently under development.

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Single Cell ICP-MS Presents Hope For More Effective Ovarian Cancer Treatments

Watch our video to learn how Dr. Lauren Amble, Staff Scientist at the NIH is using Single Cell ICP-MS to investigate cellular uptake of metal-based chemotherapy in individual ovarian cancer cells.

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Cisplatin Efficacy: The Importance of Measuring Uptake in Cancer Cells

Read about the benefits of Single Cell ICP-MS in analyzing cisplatin uptake in the June Issue of Cancer Research from Technology Networks.

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Single Cell ICP-MS: Quantification of Metals at the Cellular Level

Quantifying metal content in an individual cell can offer insight into understanding the uptake and elimination mechanisms of metals and/or metal-containing nanoparticles by individual cells.

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