We offer a wide range of 32P and 35S labeled nucleotides, stocked at different concentrations and specific activities to meet your application. Use to detect specific nucleic acid sequences.
Our labeled nucleotides may be incorporated by a variety of methods including in vitro transcription with SP6, T3 or T7 RNA polymerase, 3' end labeling with terminal deoxynucleotidyl transferase (TdT), T4 DNA polymerase or T7 DNA polymerase, random primed DNA labeling with Klenow fragment, cDNA labeling with AMV or M-MuLV reverse transcriptase, nick translation labeling with DNAse 1 and DNA Polymerase 1, and PCR labeling with thermophilic DNA polymerases like Taq or Pfu.
The resulting labeled probes may then be used in applications such as in situ hybridization, microarray analysis, DNA sequencing, southern blotting, and northern blotting.
3’ dATP (Cordycepin) is used in 3' end labeling of DNA using terminal transferase (TdT). It is a chain terminator, because it is deoxy- (missing an oxygen) at the 3’ position. It is often used in place of ddATP.