LANCE TR-FRET

OF

A simple, highly-sensitive, and highly-reproducible platform for detection, quantitation and screening in microplate format. From biomarker detection assays to cAMP/GPCR studies to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET technologies provide:

  • Time-resolved measurement for clearer signal resolution
  • Homogeneous (no wash steps, no separation steps)
  • Robust sensitivity
  • Long signal stability
  • Highly reproducible results

LANCE europium chelates are optimized for assay performance, signal level, energy transfer efficiency, background and sensitivity of the assay to interferences. The results are a superior S:B ratio and a very sensitive TR-FRET assay. You use less label and get more efficient use of assay reagents.

We also offer acceptor dyes that decrease steric hindrance while maximizing signal for labeled substrates, as well as those that provide a broad, antenna-like structure to help align fluorophores for optimal TR-FRET signal, making them excellent for measuring large binding interactions.

  • Products and Services

  • Resources, Events & More

Narrow your results
Antibody Conjugates
  • Anti-6X His (5)
  • Anti-acetyl-lysine (3)
  • Anti-c-Myc (4)
  • Anti-FITC (6)
  • Anti-FLAG (7)
  • Anti-GST (5)
  • Anti-H3K27ac (3)
  • Anti-H3K27me2 (3)
  • Anti-H3K27me3 (3)
  • Anti-H3K36me2 (3)
  • Anti-H3K4 (3)
  • Anti-H3K4me1-2 (3)
  • Anti-H3K9/H3K27 (3)
  • Anti-H3K9ac (3)
  • Anti-H3K9me2 (3)
  • Anti-H4R3me (3)
  • Anti-HA (3)
  • Anti-human IgG (7)
  • Anti-methyl arginine (3)
  • Anti-mouse IgG (7)
  • Anti-O-linked-GlcNAc (3)
  • Anti-P-Akt (2)
  • Anti-P-PKA (2)
  • Anti-P-Ser (2)
  • Anti-P-Ser 14-3-3 (2)
  • Anti-P-Ser 14-3-3 4E2 (2)
  • Anti-P-Ser PKC (2)
  • Anti-P-Ser/Thr-Phe (2)
  • Anti-P-Ser/Thr-Pro (2)
  • Anti-P-Tyr-100 (4)
  • Anti-p53K382ac (3)
  • Anti-phospho-4EBP1 (3)
  • Anti-phospho-Acetyl-CoA-Carboxylase (3)
  • Anti-phospho-ATF-2 (3)
  • Anti-phospho-Crosstide (3)
  • Anti-phospho-glycogen synthase (3)
  • Anti-phospho-Histone H3 (7)
  • Anti-phospho-I?Ba (3)
  • Anti-phospho-MBP (3)
  • Anti-phospho-MEK1/2 (3)
  • Anti-phospho-mTOR (3)
  • Anti-phospho-p38a (3)
  • Anti-phospho-p70S6K (4)
  • Anti-phospho-PKC (3)
  • Anti-phospho-PLK (4)
  • Anti-phospho-rpS6 (3)
  • Anti-phospho-threonine (2)
  • Anti-phospho-Threonine-Proline (2)
  • Anti-phospho-Topo IIa (3)
  • Anti-phospho-Tyrosine Hydroxylase (3)
  • Anti-rabbit IgG (7)
  • Biotin (1)
  • Protein G (4)
  • PT66 (3)
  • PY20 (3)
  • Streptavidin (8)
Application
  • cAMP (7)
  • ELISA (153)
  • Enzymatic (18)
  • Epigenetics (42)
  • Kinase (171)
  • Protein-protein interactions (48)
Assay Points
  • 500 (44)
  • 10000 (44)
Assay Target
  • Adiponectin (3)
  • Albumin (3)
  • Aβ1-40 (3)
  • CCL2 (4)
  • CCL4 (3)
  • CCL5 (2)
  • CHO HCP (2)
  • CHO-P (2)
  • CRP (2)
  • CXCL10 (3)
  • CXCL9 (3)
  • E.coli HCP (3)
  • ErbB2 (3)
  • Frataxin (3)
  • GLP-1 (3)
  • Glucagon (3)
  • IFNα (2)
  • IFNγ (5)
  • IgG (9)
  • IL17A (2)
  • IL1α (2)
  • IL1β (4)
  • IL2 (2)
  • IL4 (1)
  • IL5 (2)
  • IL6 (6)
  • IL8 (2)
  • Insulin (3)
  • LC3B (3)
  • Leptin (5)
  • PAI-1 (3)
  • Protein A (2)
  • Tau (3)
  • TNFα (2)
  • Transferrin (3)
  • VEGF-A (5)
Assay Target Class
  • Antibody (9)
  • Cytokine (45)
  • Hormone (11)
  • Peptide (9)
  • Protein (37)
Automation Compatible
  • Yes (417)
Detection Method
  • Time-Resolved Fluorescence (TRF) (347)
  • TR-FRET (347)
Experimental Type
  • In vitro (420)
Fluorophore
  • Eu-W1024 (175)
  • Eu-W1024-ITC (2)
  • Eu-W1284-Iodoacetamido (2)
  • Eu-W8044 (2)
  • Eu-W8044-DTA (1)
  • SureLight APC (7)
  • SureLight-APC (2)
  • ULight (107)
Format
  • Microplates (423)
Molecular Modification
  • Acetylation (18)
  • GlcNAc (3)
  • Methylation (27)
  • Phosphorylation (169)
Product Family
  • LANCE (75)
  • LANCE SureLight (9)
  • LANCE Ultra (242)
Target Species
  • CHO (4)
  • Human (84)
  • Mouse (18)
  • Rabbit (3)
Therapeutic Area
  • Angiogenesis (7)
  • Autophagy (3)
  • Biologics/Bioprocess (18)
  • Cancer (9)
  • Central Nervous System (8)
  • CNS & Neurologic (1)
  • Inflammation (48)
  • Metabolic (20)
Volume
  • 0-50 (216)
  • 51-100 (38)
  • 201-250 (1)

"LANCE TR-FRET" 1-50 of 452 Products and Services

Sort
Per Page

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36), bulk orders

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM

Product not available online

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP), bulk orders

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK

Product not available online

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP), 100 μg

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK

List Price: 5700.00 USD

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 8200.00 USD

LANCE Ultra Europium-anti-phospho-CREB (Ser133), bulk orders

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.

Product not available online

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody, bulk orders

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG

Product not available online

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody, bulk orders

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

Product not available online

LANCE Ultra Europium-anti-phospho-mTOR (Ser2448) Antibody, 100 µg

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from mTOR phosphorylated at Ser2448. mTOR peptides can be phosphorylated by kinases MST1, p70 S6K and others. Sequence motif recognized: RSRTRTDSYSAGQSV

List Price: 6600.00 USD

IFN-γ (mouse) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra Mouse IFN-γ Detection Kit is designed for detection and quantitation of mouse interferon γ in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

List Price: 10000.00 USD

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21), bulk orders

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG

Product not available online

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 8200.00 USD

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody, 10 µg

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL

List Price: 1926.00 USD

LANCE Ultra Europium-anti-P-Tyrosine (PT66) Antibody, 100 µg

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 1399.00 USD

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36), 10 μg

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM

List Price: 1358.00 USD

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody, 10 µg

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 3284.00 USD

LANCE Ultra Protein A, lyophilized (0.3u

LANCE Ultra Protein A, lyophilized (0.3u

List Price: 226.00 USD

LANCE Eu-anti-6xHis antibody, (LQC)

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

Product not available online

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody, bulk orders

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP

Product not available online

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody, bulk orders

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG

Product not available online

LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody, bulk orders

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.

Product not available online

LANCE Ultra MMP, lyophilized (0.3ug)

LANCE Ultra MMP, lyophilized (0.3ug)

List Price: 226.00 USD

LANCE Eu-W1024 Anti-c-Myc, 10 µg

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.

List Price: 222.00 USD

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody, 1.7 μg

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE

List Price: 1444.00 USD

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36), 100 μg

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM

List Price: 4409.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody, 10 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 3284.00 USD

LANCE Eu-W1024 Protein G, 1mg

Europium-labeled Protein G, for capturing antibodies in LANCE® TR-FRET assays.

List Price: 8600.00 USD

SureLight Allophycocyanin-anti-GST antibody, 1 mg, lyophilized

APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.

List Price: 468.00 USD

LANCE Ultra ULight™-MBP peptide, 0.125 nmole

MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.

List Price: 425.00 USD

LANCE Eu-W1024 anti-FLAG, 1 mg

Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.

List Price: 8500.00 USD

CXCL9/MIG (human), lyophilized (LANCE)

Human CXCL9 standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human CXCL9/MIG Detection Kit, but can be ordered separately.

List Price: 226.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66), 50 µg

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 983.00 USD

TNFα (mouse) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra Mouse TNFα Detection Kit is designed for detection and quantitation of mouse tumor necrosis factor α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

List Price: 10000.00 USD

LANCE Eu-W1024 Anti-c-Myc, 50µg

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.

List Price: 1106.00 USD

LANCE® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 3204.00 USD

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody, 10 µg

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP

List Price: 1926.00 USD

LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641), 100 µg

Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL

List Price: 6700.00 USD

LANCE Ultra KinaSelect™ Ser/Thr Kit, 5 X 250 assay points

LANCE® Ultra TR-FRET Ser/Thr kinase substrate selection kit, containing five different ULight-peptide substrates and corresponding Europium anti-phospho antibodies for kinase reactions.

List Price: 1420.00 USD

LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641), bulk orders

Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL

Product not available online

LANCE cAMP kit, 10,000 points

LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.

List Price: 1580.00 USD

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody, 100 µg

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG

List Price: 6600.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.

List Price: 8200.00 USD
Protein

VEGF (human), lyophilized (LANCE)

Human transferrin standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Transferrin TR-FRET Detection Kit, but can be ordered separately.

List Price: 226.00 USD

LANCE Eu-W1024 Anti-phosphotyrosine (P-Tyr-100), 10µg

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 199.00 USD

LANCE Ultra hLeptin, lyophilized (0.3ug)

LANCE Ultra hLeptin, lyophilized (0.3ug)

List Price: 226.00 USD
LANCE biomarker detection assay principle

GLP-1 (7-36) (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human GLP-1 (7-36) Detection Kit is designed for detection and quantitation of human GLP-1 (7-36) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

List Price: 750.00 USD

IL-5 (human) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra Human IL-5 Detection Kit is designed for detection and quantitation of human interleuukin-5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

List Price: 10000.00 USD

SureLight Allophycocyanin-Streptavidin (APC-SA), protein binding assays, 30 mg, lyophilized

APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.

List Price: 10500.00 USD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide, 5 nmoles

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG

List Price: 813.00 USD

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide, 0.125 nmole

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR

List Price: 277.00 USD
LANCE biomarker detection assay principle

CCL4 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human C-C Motif Chemokine 4/Macrophage Inflammatory Protein-1beta in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

List Price: 750.00 USD

"LANCE TR-FRET" 1-50 of 452 Products and Services

Per Page