LANCE TR-FRET

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LANCE^® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

Homogeneous assay format (no wash steps, no separation steps)
Reduced background leading to better S:B
Large Stoke’s shift
Wider dynamic range
Extended signal stability
Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE^® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Biochemical Assays

Biochemical assays remain an important tool for the drug discovery process. Whether you need a binding assay, kinase assay, other enzymatic assay, or would like to build your own assay utilizing our Toolbox reagents, LANCE has a variety of biochemical assays available.

Suggested Products

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac), 10 µg LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36), 10 μg LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide, 0.5 nmole ULight anti-FLAG, 1 nmol LANCE Eu-W1024 Anti-Human IgG, 10µg LANCE Eu-W1024 Anti-6xHis, 10 µg LANCE Eu-W1024 Streptavidin, 50µg

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:

Angiogenesis
Animal Health
Autophagy
Biologics/Bioprocess
Cancer
Cardiovascular
Central nervous system
Inflammation
Metabolic

Suggested Products

PD-L1 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points PD-1 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points TNFα (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points IL-1β (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points IFN-γ (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

Assays for Cell Signaling

Breakthrough discoveries in cell signaling and cell signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles. And LANCE helps you do just that. With a wide selection of phospho- and total protein detection kits available, we are here to help you better understand the intracellular pathways of phosphorylated proteins.

Suggested Products

Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points Phospho-NFκB (Ser536) LANCE Ultra TR-FRET Detection Kit, 500 assay points Phospho-BTK (Tyr223) LANCE Ultra TR-FRET Detection Kit, 500 assay points Phospho-AKT 1/2/3 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

Suggested Products

Custom Assay Development Services Custom Reagent Labeling - LANCE

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-12 of 365 Products & Services

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Quick View

LANCE Ultra m/rIL17A, lyophilized(1.0ug)

TRF1514S

LANCE Ultra m/rIL17A, lyophilized(1.0ug)

List Price : 250.00 USD
Quick View

COL1A1 (human), lyophilized (LANCE)

TRF1371S

Human COL1A1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COL1A1 TR-FRET Detection Kit, but can also be ordered separately.

List Price : 308.00 USD
Quick View

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

TRF0200-C , TRF0200-D , TRF0200-M

Monoclonal antibody labeled with the LANCE^® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.

Price Range : 1160.00 USD - 5000.00 USD
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PD-L1 (human) LANCE Ultra TR-FRET Detection Kit

TRF1355M , TRF1355C

The LANCE® Ultra Human PD-L1 Detection Kit is designed for detection and quantitation of human PD-L1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Price Range : 806.00 USD - 10700.00 USD
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LANCE Ultra ULight™-Crosstide

TRF0106-M , TRF0106-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight^™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.

Price Range : 330.00 USD - 900.00 USD
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Oncostatin M (human) LANCE Ultra TR-FRET Detection Kit

TRF1341C , TRF1341M

The LANCE® Ultra Human Oncostatin M Detection Kit is designed for detection and quantitation of human OSM in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Price Range : 830.00 USD - 11100.00 USD
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LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

TRF0125-M , TRF0125-C , TRF0125-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight^™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: CSERRPHFPQFSYSASGTAR

Price Range : 306.00 USD - 1250.00 USD
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MMP-8 (human), lyophilized (LANCE)

TRF1374S

Human MMP-8 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra MMP-8 TR-FRET Detection Kit, but can be ordered separately.

List Price : 317.00 USD
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IGF-1 (human), lyophilized (LANCE)

TRF1236S

Human IGF-1 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra IGF-1 TR-FRET Detection Kit, but can be ordered separately.

List Price : 258.00 USD
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IL-28B (human) LANCE Ultra TR-FRET Detection Kit

TRF1365C , TRF1365M

The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Price Range : 930.00 USD - 12400.00 USD
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Nidogen 1 (human), lyophilized (LANCE)

TRF1367S

Human Nidogen 1 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra Nidogen 1 TR-FRET Detection Kit, but can be ordered separately.

List Price : 317.00 USD
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CCL4 (human) LANCE Ultra TR-FRET Detection Kit

TRF1258M , TRF1258C

The LANCE^® Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human C-C Motif Chemokine 4/Macrophage Inflammatory Protein-1beta in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Price Range : 830.00 USD - 11100.00 USD