Kinase

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Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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  • Accessories (7)
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Antibody conjugates
  • Anti-P-Akt (1)
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  • Enzymatic (1)
  • Kinase (246)
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Assay Points
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Assay Target
  • 4EBP1 (5)
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  • Topo IIα (4)
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  • β-Catenin (1)
Assay Target Class
  • Phosphoprotein (243)
  • Protein (10)
Automation Compatible
  • Yes (327)
Buffer
  • Aqueous (2)
  • Tricine (17)
  • Tricine with stabilizer and dye (14)
Concentration
  • 10 mCi/mL (12)
  • 12.5 mCi/mL (4)
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Detection Method
  • Absorbance (1)
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  • Time-Resolved Fluorescence (TRF) (64)
  • TR-FRET (57)
Experimental type
  • In vitro (279)
Fluorophore
  • Eu-N1 (6)
  • Eu-W1024 (33)
  • SureLight-APC (1)
  • ULight (23)
Format
  • Microplates (320)
Label Position
  • alpha (2)
  • gamma (35)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
Molecular modification
  • Phosphorylation (235)
Nucleotide
  • ATP (30)
  • dTTP (1)
  • GTP (6)
Product Brand Name
  • Alpha SureFire Ultra Multiplex (14)
  • AlphaLISA SureFire Ultra (74)
  • AlphaPlex (14)
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  • AlphaScreen SureFire (161)
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  • NEN (4)
  • Victor (3)
Radionuclide
  • 32P (23)
  • 33P (4)
  • 35S (10)
Radioisotope
  • P-32 (23)
  • P-33 (4)
  • S-35 (10)
Target Species
  • Human (229)
Volume
  • 0-50 (141)
  • 51-100 (21)
  • 201-250 (16)
  • 451-500 (68)
  • Over 500 (64)

"Kinase" 1-50 of 371 Products and Services

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EnSight Multimode Plate Reader

The EnSight Multimode Plate Reader is the first benchtop system to offer well-imaging alongside label-free and labeled detection technologies.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pY701, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
List Price : 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
List Price : 26700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pS727 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.
List Price : 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
List Price : 26800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
List Price : 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
List Price : 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
List Price : 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
List Price : 8600.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-cJun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser63) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
List Price : 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pY701 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
List Price : 461.00 USD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.
Price Range : 1407.00 USD - 15200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology.
List Price : 15200.00 USD
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 10,000 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.
List Price : 6800.00 USD
SureFire principle

AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
List Price : 6800.00 USD
SureFire principle

AlphaScreen SureFire HV NFkΒ p65 (p-Ser536) Assay Kit - 96-well format

The AlphaScreen® SureFire®p-NF-κB p65 (p-Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NF-κB p65 (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
List Price : 422.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit, 10,000 points

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
List Price : 6800.00 USD
SureFire principle

AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology.
List Price : 15200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 1407.00 USD - 15200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
List Price : 461.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 500 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.
List Price : 1407.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-p38 MAPK Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.
List Price : 6800.00 USD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
List Price : 1407.00 USD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 1407.00 USD - 15200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
List Price : 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26700.00 USD
alphascreen surefire total

AlphaScreen SureFire Monoclonal Total ERK HV, 100 Assay Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
List Price : 422.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-cJun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser73) in cellular lysates using Alpha Technology.
List Price : 1407.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 422.00 USD - 16700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
List Price : 26700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
List Price : 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
List Price : 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 26800.00 USD
SureFire principle

AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology.
List Price : 1407.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 8600.00 USD
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology.
List Price : 6200.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 983.00 USD - 8600.00 USD

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 170.00 USD - 13000.00 USD
SureFire principle

AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD

"Kinase" 1-50 of 371 Products and Services

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