Kinase

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Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Antibody conjugates
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Assay Target Class
  • Phosphoprotein (283)
  • Protein (10)
Automation Compatible
  • Yes (375)
Buffer
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  • Microplates (367)
Label Position
  • alpha (2)
  • gamma (35)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
Molecular modification
  • Phosphorylation (274)
Nucleotide
  • ATP (30)
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Radionuclide
  • 32P (23)
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Radioisotope
  • P-32 (23)
  • P-33 (4)
  • S-35 (10)
Target Species
  • Human (269)
Volume
  • 0-50 (151)
  • 51-100 (21)
  • 201-250 (16)
  • 451-500 (68)
  • Over 500 (64)

"Kinase" 1-50 of 420 Products and Services

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EnSight Multimode Plate Reader

The EnSight Multimode Plate Reader is the first benchtop system to offer well-imaging alongside label-free and labeled detection technologies.

AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total p38 MAPK Assay Kit

AlphaLISA® SureFire® Ultra Total p38 MAPK assays are sandwich immunoassays for quantitative detection of p38 MAPK (phosphorylated and unphosphorylated) in cellular lysates using Alpha no-wash technology. This assay can be used for normalization of phosphorylation and other studies.

List Price: 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pS727, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

List Price: 26800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pS727, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

List Price: 8600.00 USD

LANCE® Eu-W1024 phospho-serine/threonine-phenylalanine antibody, 10 µg

Europium-labeled anti-phospho-serine/threonine-phenylalanine antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 3806.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pS727 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ Multiplex p-AKT (Ser473) (directly coupled) HV Kit, 100 Assay Points

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).

List Price: 461.00 USD

LANCE Eu-W1024 phospho-serine/threonine-proline antibody, 10 µg

Europium-labeled anti-phospho-serine/threonine-proline antibody, for use in LANCE® TR-FRET kinase assays.

List Price: 585.00 USD
SureFire principle

AlphaScreen SureFire mTOR (p-Ser2448) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

List Price: 1558.00 USD

LANCE Ultra Europium-anti-phospho-ATF-2 (Thr53) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human Activating Transcription Factor 2 (ATF-2; Swiss-Prot entry P15336) phosphorylated at Thr53. Thr53 of ATF-2 is phosphorylated by JNK and p38 kinases. Sequence motif recognized: VADQTPTPTRFLK

List Price: 1926.00 USD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 1543.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ Multiplex p-ERK (Eu) (directly coupled) Kit, 500 Assay Points

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).

List Price: 1558.00 USD
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 10,000 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.

List Price: 6800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pS727, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.

List Price: 1423.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ Multiplex p-ERK (Eu) (directly coupled) Kit, 50,000 Assay Points

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).

List Price: 26800.00 USD
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 500 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.

List Price: 1543.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pY701, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml, 250 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.

List Price: 554.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
SureFire principle

AlphaScreen SureFire p-Smad3 (Ser423/425) research kit, 10,000 points

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology.

List Price: 6800.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Ser473) research kit, 50,000 points

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 16700.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.

List Price: 15400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD

LANCE Ultra Europium-anti-phospho-MAP Kinase p38-alpha (Thr180/Tyr182)

Europium-labeled mouse monoclonal antibody recognizing human MAP kinase p38-alpha phosphorylated at Thr180 and Tyr182. Sequence motif recognized: DEM(pT)G(pY)VATRW

List Price: 1926.00 USD
SureFire principle

AlphaScreen SureFire mTOR (p-Ser2448) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD

AlphaScreen Phosphotyrosine (PT66) Assay Kit, 50,000 assay points

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.

List Price: 13000.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.

List Price: 1558.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD

ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml EasyTide Lead, 500 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.

List Price: 768.00 USD
List Price: 17400.00 USD
alphascreen surefire total

AlphaScreen SureFire Monoclonal Total ERK, 50,000 Assay Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.

List Price: 16700.00 USD

"Kinase" 1-50 of 420 Products and Services

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