Kinase

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Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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  • Accessories (6)
  • Instruments (1)
  • Reagents (259)
Antibody conjugates
  • Anti-P-PKA (1)
  • Anti-P-Ser (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-P-Tyr-100 (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-Histone H3 (2)
  • Anti-phospho-I?Ba (1)
  • Anti-phospho-MEK1/2 (1)
  • Anti-phospho-p70S6K (1)
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Application
  • Enzymatic (1)
  • Kinase (153)
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  • Multiplexing (14)
  • Protein Analysis & Detection (115)
Assay Points
  • 100 (26)
  • 1000 (1)
  • 10000 (86)
  • 500 (85)
  • 50000 (87)
Assay Target
  • 4EBP1 (3)
  • Akt1 (2)
  • Akt1/2 (3)
  • Akt1/2/3 (19)
  • ALK (2)
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  • Elk1 (1)
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  • ERK1/2 (21)
  • GAPDH (1)
  • GSK3α (1)
  • GSK3β (1)
  • Histone H3 (1)
  • Hsp27 (1)
  • IGFR (1)
  • IKKα (8)
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  • IκBα (3)
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  • mTOR (5)
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  • p38 MAPK (4)
  • p53 (2)
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  • SLP-76 (4)
  • SMAD1 (5)
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  • SMAD3 (5)
  • STAT1 (9)
  • STAT3 (6)
  • STAT5 (5)
  • Topo IIα (2)
  • VEGF2 Receptor (2)
  • β-Catenin (1)
Assay Target Class
  • Phosphoprotein (156)
  • Protein (10)
Automation Compatible
  • Yes (225)
Buffer
  • Aqueous (2)
  • Tricine (17)
  • Tricine with stabilizer and dye (12)
Concentration
  • 10 mCi/mL (13)
  • 12.5 mCi/mL (4)
  • 2 mCi/mL (1)
  • 5 mCi/mL (2)
Detection Method
  • Absorbance (1)
  • Alpha (171)
  • DELFIA TRF (6)
  • Fluorescence (1)
  • Image-based Cytometry (1)
  • Label-free (1)
  • Luminescence (1)
  • Radiometric (35)
  • Time-Resolved Fluorescence (TRF) (51)
  • TR-FRET (44)
Experimental type
  • In vitro (188)
Fluorophore
  • Eu-N1 (6)
  • Eu-W1024 (24)
  • SureLight-APC (1)
  • ULight (19)
Format
  • Microplates (220)
Label Position
  • alpha (2)
  • gamma (33)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
Molecular modification
  • Phosphorylation (142)
Nucleotide
  • ATP (29)
  • GTP (6)
Product Brand Name
  • Alpha SureFire Ultra Multiplex (14)
  • AlphaLISA SureFire Ultra (65)
  • AlphaPlex (14)
  • AlphaScreen (4)
  • AlphaScreen SureFire (83)
  • DELFIA (6)
  • EasyTide (31)
  • EnSight (1)
  • EnSpire (2)
  • EnVision (2)
  • LANCE (6)
  • LANCE SureLight (1)
  • LANCE Ultra (37)
  • NEN (4)
  • Victor (2)
Radionuclide
  • 32P (23)
  • 33P (2)
  • 35S (10)
Radioisotope
  • P-32 (23)
  • P-33 (2)
  • S-35 (10)
Target Species
  • Human (142)
Volume
  • 0-50 (89)
  • 51-100 (4)
  • 201-250 (16)
  • 451-500 (47)
  • Over 500 (42)

"Kinase" 1-50 of 266 Products and Services

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EnSight Multimode Plate Reader

The EnSight Multimode Plate Reader is the first benchtop system to offer well-imaging alongside label-free and labeled detection technologies.

ATP, [γ-32P]- 3000Ci/mmol 5mCi/ml EasyTide Lead, 500 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
List Price : 659.00 USD

ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml EasyTide Lead, 1 mCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
List Price : 1143.00 USD

ATP, [γ-32P]- 3000Ci/mmol 10mCi/ml EasyTide Lead, 1 mCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
List Price : 873.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.
Price Range : 381.00 USD - 837.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 419.00 USD - 1885.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

LANCE Ultra ULight™-Crosstide

Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
Price Range : 307.00 USD - 838.00 USD

ATP, [γ-32P]- 3000Ci/mmol 10mCi/ml EasyTide Lead, 100 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
List Price : 366.00 USD

ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml EasyTide Lead, 250 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
List Price : 538.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

LANCE Ultra KinaSelect™ Ser/Thr Kit, 5 X 250 assay points

LANCE® Ultra TR-FRET Ser/Thr kinase substrate selection kit, containing five different ULight-peptide substrates and corresponding Europium anti-phospho antibodies for kinase reactions.
List Price : 1463.00 USD

LANCE Ultra ULight™-CREBtide

CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight™ dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 231.00 USD - 838.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.
List Price : 1605.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 693.00 USD - 1779.00 USD
SureFire principle

AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

The AlphaScreen® SureFire® p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit

The AlphaScreen® SureFire® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Ser1106) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD

LANCE® Eu-W1024 phospho-PKA sustrate antibody, 10 µg

Europium-labeled anti-phospho-PKA substrate antibody, for use in LANCE® TR-FRET kinase assays.
List Price : 2629.00 USD
Price Range : 386.00 USD - 17900.00 USD
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire Histone H3 (p-Ser10) Assay Kit

The AlphaScreen® SureFire® p-Histone H3 (Ser10) assay is a sandwich immunoassay for quantitative detection of phospho-Histone H3 (phosphorylated on Ser10) in cellular lysates using Alpha Technology.
Price Range : 1448.00 USD - 15600.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD
SureFire principle

AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

The AlphaScreen® SureFire® p-STAT3 (p-Ser705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Ser705) in cellular lysates using Alpha Technology.
Price Range : 435.00 USD - 17200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) HV Assay Kit - 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
List Price : 475.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-cJun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser63) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL
Price Range : 1984.00 USD - 6800.00 USD
SureFire principle

AlphaScreen SureFire SMAD1 (p-Ser463;Ser465) Assay Kit

The AlphaScreen® SureFire® p-SMAD1 (p-Ser463/465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/465) in cellular lysates using Alpha Technology.
Price Range : 1588.00 USD - 17200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 285.00 USD - 1164.00 USD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 298.00 USD - 813.00 USD
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 10,000 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.
List Price : 7000.00 USD
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire mTOR (p-Ser2448) Assay Kit

The AlphaScreen® SureFire® p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
List Price : 8900.00 USD

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1399.00 USD - 4541.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD

ATP, [γ-32P]- 3000Ci/mmol 10mCi/ml , 1 mCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
List Price : 835.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-cJun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser73) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 1449.00 USD - 15700.00 USD

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
Price Range : 381.00 USD - 838.00 USD

"Kinase" 1-50 of 266 Products and Services

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