PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

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Application
  • Immunohistochemistry (IHC) (25)
  • In situ hybridization (ISH) (25)
  • Molecular Biology (14)
Buffer
  • Tricine (2)
  • Tricine with stabilizer and dye (12)
Concentration
  • 1 mm (1)
  • 10 mCi/mL (10)
  • 12.5 mCi/mL (1)
Detection Method
  • Chromogenic (6)
  • Fluorescence (69)
  • Radiometric (23)
Fluorophore
  • Coumarin (1)
  • Cy3 (19)
  • Cy3.5 (1)
  • Cy5 (16)
  • Cy5.5 (1)
  • Fluorescein (17)
  • Lissamine (1)
  • R110 (3)
  • R6G (3)
  • ROX (2)
  • TAMRA (4)
  • Texas Red (3)
  • TMR (5)
Format
  • Slides (25)
Label Position
  • alpha (11)
  • gamma (3)
Nucleotide
  • ATP (5)
  • CTP (5)
  • dATP (8)
  • dCTP (12)
  • ddATP (6)
  • ddCTP (6)
  • ddGTP (6)
  • ddUTP (7)
  • dGTP (4)
  • dTTP (1)
  • dUTP (9)
  • GTP (3)
  • UTP (11)
Product Brand Name
  • Ancillary Products (2)
  • EasyTide (7)
  • NEN (2)
  • NEN Radiochemicals (5)
  • Opal (1)
  • TSA (12)
  • TSA Plus (14)
Radionuclide
  • 33P (12)
  • 35S (2)
Radioisotope
  • P-33 (12)
  • S-35 (2)
Type
  • Opal 520 (1)
  • Opal 540 (1)
  • Opal 570 (1)
  • Opal 620 (1)
  • Opal 650 (1)
  • Opal 690 (1)
Volume
  • 0-50 (14)
  • 51-100 (8)
  • 201-250 (11)
Volume Capacity
  • 150 µL (2)
  • 25 nmol (2)
  • 75 nmol (1)

"In Situ Hybridization" 1-50 of 115 Products and Services

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PerkinElmer

Cyanine 3-dGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 451.00 USD
PerkinElmer

Cyanine 5-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 483.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 419.00 USD - 1885.00 USD
PerkinElmer

Cyanine 3-UTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 483.00 USD
PerkinElmer

Biotin-11-dGTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 321.00 USD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 517.00 USD - 2139.00 USD

Cyanine 5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 453.00 USD

CTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Cytidine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 438.00 USD - 2351.00 USD
PerkinElmer

Cyanine Smart Pack dUTP

Each Cyanine Smart Pack dCTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled deoxycytidine triphosphate (dCTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 3630.00 USD
PerkinElmer

Biotin-11-ddCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 439.00 USD
PerkinElmer

Cyanine 5-UTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 493.00 USD

TSA Plus Cyanine 5.5

TSA® Plus Cyanine 5.5 (Cy5.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5.5 in the localized area of the probe or antibody.
Price Range : 240.00 USD - 1919.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 693.00 USD - 1857.00 USD
PerkinElmer

TSA Plus Cyanine 3 System, for 50-150 Slides

TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
List Price : 438.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 693.00 USD - 1779.00 USD
PerkinElmer

Biotin-11-ddATP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 439.00 USD
Amplification Diluent

TSA Cyanine 5 Tyramide Reagent Pack, Reagents for 50-150 Slides

TSA® Cyanine 5 (Cy5) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
List Price : 373.00 USD

UTPαS, [35S]- 800Ci/mmol, 40mCi/ml, 1 mCi

Uridine triphosphate, labeled on the alpha phosphate group with 35S.
List Price : 1668.00 USD
PerkinElmer

TSA Biotin System

TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 364.00 USD - 1214.00 USD

Fluorescein-12-ATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 458.00 USD - 2343.00 USD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 824.00 USD - 2546.00 USD

Texas Red-5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

TSA Plus DIG Evaluation Kit

TSA® Plus DIG detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra DIG in the localized area of the probe or antibody.
Price Range : 232.00 USD - 1534.00 USD
PerkinElmer

TSA Plus DNP (HRP)

TSA® Plus DNP (HRP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP hroseradish peroxidase, with appropriate substrate.
Price Range : 275.00 USD - 491.00 USD
PerkinElmer

Cyanine Smart Pack UTP

Each Cyanine Smart Pack UTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled uridine triphosphate (UTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 3876.00 USD
PerkinElmer

Biotin-11-dATP 1mM 25nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 321.00 USD
PerkinElmer

Diethylaminocoumarin- 5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

Opal Multiplex Manual IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 952.00 USD - 1204.00 USD
PerkinElmer

TSA Plus Biotin

TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 409.00 USD - 1534.00 USD
PerkinElmer

Cyanine 3-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 453.00 USD

dTTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Deoxythymidine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 2618.00 USD
PerkinElmer

ROX-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD
PerkinElmer

Biotin-11-dCTP 5mM 1,000nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 10500.00 USD
PerkinElmer

TSA Cyanine 5 System, for 50-150 Slides

TSA® Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody.
List Price : 467.00 USD
PerkinElmer

Biotin-16 UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 324.00 USD
PerkinElmer

TSA Biotin Reagent Pack

TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 969.00 USD - 4483.00 USD
PerkinElmer

3-Amino-3-Deoxydigoxigenin-9-ddGTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 439.00 USD
TSA Tetramethylrhodamine System

TSA Tetramethylrhodamine System, for 100-300 Slides

TSA® tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
List Price : 820.00 USD
PerkinElmer

Cyanine 3-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 3408.00 USD
PerkinElmer

DNP-11-UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 321.00 USD
TSA Plus DNP (AP)System

TSA Plus DNP (AP)

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.
Price Range : 275.00 USD - 491.00 USD
PerkinElmer

Cyanine 5-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 514.00 USD
TSA Cyanine 3 System/Cyanine 5 System

TSA Plus Cyanine 3 and Cyanine 5 System

TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
List Price : 756.00 USD
PerkinElmer

Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

TSA Plus Cyanine 5

TSA® Plus Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody.
Price Range : 240.00 USD - 1919.00 USD
PerkinElmer

Cyanine 3-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 483.00 USD
PerkinElmer

Fluorescein-12-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD

Lissamine-5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
Opal 570 Reagent Pack

Opal Reagent Packs

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 118.00 USD - 134.00 USD

"In Situ Hybridization" 1-50 of 115 Products and Services

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