In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromgenic signal generation.

Improved limit of detection and quantitative range. Reduced exposure time for faster imaging. Excellent localization of signal to the target.

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Application
  • Immunohistochemistry (IHC) (25)
  • In situ hybridization (ISH) (25)
  • Molecular Biology (34)
Buffer
  • Tricine (3)
  • Tricine with stabilizer and dye (31)
Concentration
  • 1 mm (1)
  • 10 mCi/mL (15)
  • 12.5 mCi/mL (1)
  • 5 mm (1)
Detection Method
  • Chromogenic (6)
  • Fluorescence (74)
  • Radiometric (45)
Fluorophore
  • Coumarin (1)
  • Cy3 (19)
  • Cy3.5 (1)
  • Cy5 (17)
  • Cy5.5 (1)
  • Diethylaminocoumarin (1)
  • Fluorescein (17)
  • Lissamine (1)
  • R110 (3)
  • R6G (4)
  • ROX (3)
  • TAMRA (4)
  • Texas Red (4)
  • TMR (5)
Format
  • Slides (25)
Label Position
  • alpha (27)
  • gamma (7)
Nucleotide
  • ATP (9)
  • CTP (8)
  • dATP (13)
  • dCTP (17)
  • ddATP (7)
  • ddCTP (7)
  • ddGTP (7)
  • ddUTP (7)
  • dGTP (4)
  • dTTP (2)
  • dUTP (9)
  • GTP (5)
  • UTP (15)
Product Brand Name
  • Ancillary Products (2)
  • EasyTide (18)
  • NEN (3)
  • NEN Radiochemicals (13)
  • TSA (12)
  • TSA Plus (14)
Radionuclide
  • 33P (31)
  • 35S (3)
Radioisotope
  • P-33 (31)
  • S-35 (3)
Volume
  • 0-50 (14)
  • 51-100 (9)
  • 201-250 (11)
Volume Capacity
  • 150 µL (2)
  • 25 nmol (2)
  • 50 nmol (1)
  • 75 nmol (1)

"In Situ Hybridization" 1-50 of 142 Products and Services

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ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml, 100 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
List Price : 335.00 USD

Biotin-11-dCTP 1mM 25nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 300.00 USD

Cyanine 5-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 422.00 USD

Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 300.00 USD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 100 µCi

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 366.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml, 250 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
List Price : 554.00 USD

Fluorescein-12-GTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 306.00 USD

Biotin-11-dCTP 1mM

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 247.00 USD

TSA Plus Fluorescein

TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 233.00 USD - 1863.00 USD

Biotin-11-dATP 5mM 50nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 570.00 USD

Cyanine 5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 3185.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
List Price : 1423.00 USD

Fluorescein-12-UTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 306.00 USD

TSA Biotin System

TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 354.00 USD - 1179.00 USD

Cyanine Smart Pack dUTP

Each Cyanine Smart Pack dCTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled deoxycytidine triphosphate (dCTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 3393.00 USD

Biotin-11-ddATP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 410.00 USD

Cyanine 5-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 481.00 USD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 250 µCi

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 686.00 USD

TSA Plus DIG Evaluation Kit

TSA® Plus DIG detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra DIG in the localized area of the probe or antibody.
Price Range : 225.00 USD - 1489.00 USD

Biotin-11-dATP 5mM 75nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 808.00 USD

TSA Plus DNP (AP)

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.
Price Range : 267.00 USD - 477.00 USD

TSA Plus TMR

TSA® Plus tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
Price Range : 425.00 USD - 1863.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml, 250 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
List Price : 554.00 USD

3-Amino-3-Deoxydigoxigenin-9-dCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 300.00 USD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 1874.00 USD
Amplification Diluent

TSA Cyanine 5 Tyramide Reagent Pack, Reagents for 50-150 Slides

TSA® Cyanine 5 (Cy5) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
List Price : 362.00 USD

Anti-DNP AP Conjugate

Anti-DNP antibody conjugated to alkaline phosphatase
List Price : 321.00 USD

dCTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 100 µCi

Deoxycytidine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 400.00 USD

Biotin-11-ddCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 410.00 USD
Amplification Diluent

TSA Fluorescein Reagent Pack

TSA® fluorescein reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 636.00 USD - 2981.00 USD

Cyanine 5-ATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 452.00 USD

Cyanine 5-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 481.00 USD

DNP-11-UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 300.00 USD
TSA Cyanine 3 System

TSA Cyanine 3 System, for 50-150 Slides

TSA® Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
List Price : 453.00 USD

Biotin-11-CTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 306.00 USD

Texas Red®-5-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 410.00 USD

Biotin-16 UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 302.00 USD

TSA Plus DNP (HRP)

TSA® Plus DNP (HRP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP hroseradish peroxidase, with appropriate substrate.
Price Range : 267.00 USD - 477.00 USD

TSA Fluorescein System

TSA® fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 453.00 USD - 796.00 USD

Biotin-11-dATP 1mM 25nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 300.00 USD

Cyanine 3-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 3185.00 USD

Cyanine 5-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 452.00 USD
TSA -Plus TMR/Fluorescein System

TSA Plus Fluorescein and Tetramethylrhodamine (TMR) System

TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
List Price : 734.00 USD

GTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Guanosine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 1711.00 USD

R110-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 410.00 USD

dCTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 250 µCi

Deoxycytidine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 753.00 USD

Texas Red®-5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 300.00 USD

Fluorescein-12-dGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 300.00 USD

dTTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Deoxythymidine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 2094.00 USD

UTPαS, [35S]- 1250Ci/mmol, 12.5mCi/ml, 250 µCi

Uridine triphosphate, labeled on the alpha phosphate group with 35S.
List Price : 560.00 USD

"In Situ Hybridization" 1-50 of 142 Products and Services

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