PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

Tumor Microenvironment Opal 7 Color Image
Tumor Microenvironment Opal 7 Color Image

Stain

Simultaneously detect six biomarkers plus DAPI within a single tissue section. Similar to standard immunohistochemistry, our advanced method is accessible to many laboratories where IHC method development is performed. It allows researchers to use the best primary antibodies, even those raised in the same species, together for multiplex detection. Our staining solutions provide researchers with tools to assess multiple cellular phenotypes at the same time while retaining context provided within tissue sections.
Vectra® Polaris™ Automated Quantitative Pathology Imaging System
Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Image

Our quantitative pathology imaging systems deliver high quality data where morphological context is preserved down to the sub-cellular level, removing autofluorescence and increasing signal-to-noise. These systems detect and measure multiple biomarkers, even if overlapping, within a single IHC or IF tissue section or TMA. Low signal levels that were previously undetected can now be captured, and overall quantification is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
inform Image Analysis Software for Digital Pathology
inform Image Analysis Software for Digital Pathology

Analyze

Our software programs combine the latest technologies with algorithms and intuitive, easy-to-use interfaces, giving you the power to make new, exciting discoveries from your data. Our automated advanced image analysis software allows you to visualize, analyze, quantify and phenotype cells labeled with multiple biomarkers in situ in FFPE tissue sections.
Contract Research Services for Phenotyping In Situ
Contract Research Services for Phenotyping In Situ

Contract Research Services

Interested in testing the Phenoptics™ workflow? Our Research Services team can perform multiplexed IHC for up to seven colors (six markers plus DAPI) to help you get results.
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Application
  • Immunohistochemistry (IHC) (25)
  • In situ hybridization (ISH) (25)
  • Molecular Biology (14)
Buffer
  • Tricine (2)
  • Tricine with stabilizer and dye (12)
Concentration
  • 1 mm (1)
  • 10 mCi/mL (10)
  • 12.5 mCi/mL (1)
Detection Method
  • Chromogenic (6)
  • Fluorescence (69)
  • Radiometric (23)
Fluorophore
  • Coumarin (1)
  • Cy3 (19)
  • Cy3.5 (1)
  • Cy5 (16)
  • Cy5.5 (1)
  • Fluorescein (17)
  • Lissamine (1)
  • R110 (3)
  • R6G (3)
  • ROX (2)
  • TAMRA (4)
  • Texas Red (3)
  • TMR (5)
Format
  • Slides (25)
Label Position
  • alpha (11)
  • gamma (3)
Nucleotide
  • ATP (5)
  • CTP (5)
  • dATP (8)
  • dCTP (12)
  • ddATP (6)
  • ddCTP (6)
  • ddGTP (6)
  • ddUTP (7)
  • dGTP (4)
  • dTTP (1)
  • dUTP (9)
  • GTP (3)
  • UTP (11)
Product Brand Name
  • Ancillary Products (2)
  • EasyTide (7)
  • NEN (2)
  • NEN Radiochemicals (5)
  • Opal (1)
  • TSA (12)
  • TSA Plus (14)
Radionuclide
  • 33P (12)
  • 35S (2)
Radioisotope
  • P-33 (12)
  • S-35 (2)
Type
  • Opal 520 (1)
  • Opal 540 (1)
  • Opal 570 (1)
  • Opal 620 (1)
  • Opal 650 (1)
  • Opal 690 (1)
Volume Capacity
  • 150 µL (2)

"In Situ Hybridization" 1-50 of 115 Products and Services

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PerkinElmer

Diethylaminocoumarin- 5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD

UTPαS, [35S]- 1250Ci/mmol, 12.5mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 35S.
Price Range : 617.00 USD - 1523.00 USD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 517.00 USD - 2139.00 USD
PerkinElmer

ROX-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD

R6G-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD
PerkinElmer

Anti-DNP HRP Conjugate

Anti-DNP antibody conjugated to horseradish peroxidase
List Price : 280.00 USD

Fluorescein-12-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
TSA -Plus DIG, 50-150 slides

TSA Plus DIG Evaluation Kit

TSA® Plus DIG detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra DIG in the localized area of the probe or antibody.
Price Range : 232.00 USD - 1534.00 USD
PerkinElmer

TSA Plus Biotin

TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 409.00 USD - 1534.00 USD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 517.00 USD - 2139.00 USD
PerkinElmer

TSA Cyanine 3 Reagent Pack

TSA® Cyanine 3 (Cy3) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 373.00 USD - 1750.00 USD

Fluorescein-12-ATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

Biotin-11-ATP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 321.00 USD

Texas Red-5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

TSA Fluorescein Reagent Pack

TSA® fluorescein reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 655.00 USD - 3071.00 USD
Opal 520 Reagent Pack

Opal Reagent Packs

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 118.00 USD - 134.00 USD
PerkinElmer

R110-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD
PerkinElmer

TSA Plus Cyanine 3

TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
Price Range : 240.00 USD - 1919.00 USD
PerkinElmer

Cyanine 3-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 453.00 USD

dCTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxycytidine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 500.00 USD - 2648.00 USD
TSA Cyanine 3 System

TSA Cyanine 3 System, for 50-150 Slides

TSA® Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
List Price : 467.00 USD
PerkinElmer

Biotin-11-CTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 327.00 USD
PerkinElmer

DNP-11-dUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 321.00 USD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 824.00 USD - 2546.00 USD

Fluorescein-12-CTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 327.00 USD

Fluorescein-12-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 277.00 USD
PerkinElmer

Biotin-11-ddUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 439.00 USD
PerkinElmer

3-Amino-3-Deoxydigoxigenin-9-ddGTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 439.00 USD
TSA -Plus Tetramethylrhodamine System

TSA Plus TMR

TSA® Plus tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
Price Range : 438.00 USD - 1919.00 USD

CTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Cytidine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 438.00 USD - 2351.00 USD
PerkinElmer

Cyanine 5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 453.00 USD

R6G-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD
TSA Coumarin System (100-300 slides)

TSA Coumarin System, for 100-300 Slides

TSA® coumarin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra coumarin in the localized area of the probe or antibody.
List Price : 820.00 USD
PerkinElmer

Texas Red®-5-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 327.00 USD
PerkinElmer

R110-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD
TSA Plus DNP (AP)System

TSA Plus DNP (AP)

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.
Price Range : 275.00 USD - 491.00 USD
PerkinElmer

Cyanine 3-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 483.00 USD
PerkinElmer

Biotin-11-dATP 1mM 25nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 321.00 USD
PerkinElmer

TSA Plus Fluorescein

TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 240.00 USD - 1919.00 USD
PerkinElmer

Fluorescein-12-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 447.00 USD
PerkinElmer

TAMRA-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD
PerkinElmer

Cyanine 3-UTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 483.00 USD
Amplification Diluent

TSA Cyanine 5 Tyramide Reagent Pack, Reagents for 50-150 Slides

TSA® Cyanine 5 (Cy5) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
List Price : 373.00 USD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 419.00 USD - 1885.00 USD
PerkinElmer

Cyanine 5-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 451.00 USD
PerkinElmer

Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

TSA Plus Cyanine 3 System, for 50-150 Slides

TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
List Price : 438.00 USD
PerkinElmer

Biotin-11-dCTP 5mM 1,000nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 10500.00 USD

Lissamine-5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 321.00 USD
PerkinElmer

TAMRA-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 439.00 USD

"In Situ Hybridization" 1-50 of 115 Products and Services

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