PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

Tumor Microenvironment Opal 7 Color Image
Tumor Microenvironment Opal 7 Color Image

Stain

Simultaneously detect eight biomarkers plus DAPI within a single tissue section. Similar to standard immunohistochemistry, our advanced method is accessible to many laboratories where IHC method development is performed. It allows researchers to use the best primary antibodies, even those raised in the same species, together for multiplex detection. Our staining solutions provide researchers with tools to assess multiple cellular phenotypes at the same time while retaining context provided within tissue sections
Vectra® Polaris™ Automated Quantitative Pathology Imaging System
Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Image

Our quantitative pathology imaging systems deliver high quality data where morphological context is preserved down to the sub-cellular level, removing autofluorescence and increasing signal-to-noise. These systems detect and measure multiple biomarkers, even if overlapping, within a single IHC or IF tissue section or TMA. Low signal levels that were previously undetected can now be captured, and overall quantification is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
inform Image Analysis Software for Digital Pathology
inform Image Analysis Software for Digital Pathology

Analyze

Our software programs combine the latest technologies with algorithms and intuitive, easy-to-use interfaces, giving you the power to make new, exciting discoveries from your data. Our automated advanced image analysis software allows you to visualize, analyze, quantify and phenotype cells labeled with multiple biomarkers in situ in FFPE tissue sections.
Contract Research Services for Phenotyping In Situ
Contract Research Services for Phenotyping In Situ

Contract Research Services

Interested in testing the Phenoptics™ workflow? Our Research Services team can perform multiplexed IHC for up to seven colors (six markers plus DAPI) to help you get results.
Products & Services (102)
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1-50 of 102 Products & Services

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  • TSA -Plus Tetramethylrhodamine System

    TSA Plus TMR

    TSA® Plus tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
    Price Range : 414.00 USD - 1815.00 USD
  • PerkinElmer

    R110-ddUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • PerkinElmer

    Cyanine 5-dUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 489.00 USD
  • PerkinElmer

    TSA Fluorescein System

    TSA® fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
    Price Range : 409.00 USD - 718.00 USD
  • PerkinElmer

    Biotin-11-CTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 354.00 USD
  • TSA -Plus Cyanine 5 System

    TSA Plus Cyanine 5

    TSA® Plus Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody.
    Price Range : 223.00 USD - 1779.00 USD
  • Tissue image

    Opal Multiplex Manual IHC Kit 50 slides

    Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
    Price Range : 1030.00 USD - 1302.00 USD
  • Fluorescein-12-dGTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 347.00 USD
  • PerkinElmer

    R110-ddGTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • TSA -Plus Fluorescein System

    TSA Plus Fluorescein

    TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
    Price Range : 223.00 USD - 1779.00 USD
  • PerkinElmer

    Fluorescein-12-ddUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 483.00 USD
  • PerkinElmer

    TSA Fluorescein Reagent Pack

    TSA® fluorescein reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
    Price Range : 579.00 USD - 2715.00 USD
  • PerkinElmer

    Cyanine Smart Pack dCTP

    Each Cyanine Smart Pack dCTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled deoxycytidine triphosphate (dCTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
    List Price : 3926.00 USD
  • PerkinElmer

    Fluorescein-12-GTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 354.00 USD
  • TSA -Plus Cyanine 3 System

    TSA Plus Cyanine 3

    TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
    Price Range : 223.00 USD - 1779.00 USD
  • TSA Tetramethylrhodamine System

    TSA Tetramethylrhodamine System, for 100-300 Slides

    TSA® tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
    List Price : 718.00 USD
  • Fluorescein-12-ATP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 347.00 USD
  • PerkinElmer

    TSA Plus Cyanine 5.5

    TSA® Plus Cyanine 5.5 (Cy5.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5.5 in the localized area of the probe or antibody.
    Price Range : 225.00 USD - 1797.00 USD
  • PerkinElmer

    ROX-ddATP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • PerkinElmer

    Biotin-11-dGTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 347.00 USD
  • PerkinElmer

    Anti-DNP HRP Conjugate

    Anti-DNP antibody conjugated to horseradish peroxidase
    List Price : 289.00 USD
  • PerkinElmer

    Cyanine 5-dCTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 3685.00 USD
  • PerkinElmer

    Cyanine Smart Pack UTP

    Each Cyanine Smart Pack UTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled uridine triphosphate (UTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
    List Price : 4476.00 USD
  • PerkinElmer

    TSA Cyanine 3 Reagent Pack

    TSA® Cyanine 3 (Cy3) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra Cy3 in the localized area of the probe or antibody.
    Price Range : 330.00 USD - 1547.00 USD
  • PerkinElmer

    Cyanine 3-dUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 489.00 USD
  • PerkinElmer

    TSA Biotin System

    TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
    Price Range : 319.00 USD - 1063.00 USD
  • PerkinElmer

    Anti-DNP AP Conjugate

    Anti-DNP antibody conjugated to alkaline phosphatase
    List Price : 340.00 USD
  • PerkinElmer

    R110-ddCTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • PerkinElmer

    TSA Biotin Reagent Pack

    TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
    Price Range : 857.00 USD - 3964.00 USD
  • PerkinElmer

    Biotin-11-ddUTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 474.00 USD
  • Cyanine 5-dCTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 489.00 USD
  • PerkinElmer

    TSA Plus DNP (AP)

    TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.
    Price Range : 289.00 USD - 516.00 USD
  • Opal 650 Reagent Pack

    Opal Reagent Packs

    Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
    Price Range : 127.00 USD - 147.00 USD
  • PerkinElmer

    TAMRA-ddGTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • PerkinElmer

    Cyanine 5-dATP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 488.00 USD
  • TSA Coumarin System (100-300 slides)

    TSA Coumarin System, for 100-300 Slides

    TSA® coumarin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra coumarin in the localized area of the probe or antibody.
    List Price : 725.00 USD
  • Texas Red®-5-dCTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 347.00 USD
  • Fluorescein-12-dUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 285.00 USD
  • PerkinElmer

    3-Amino-3-Deoxydigoxigenin-9-dCTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 347.00 USD
  • PerkinElmer

    Biotin-11-GTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 347.00 USD
  • Texas Red-5-dUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 347.00 USD
  • PerkinElmer

    Biotin-11-ddGTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 474.00 USD
  • PerkinElmer

    Cyanine 3-CTP, 100 nmol

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 523.00 USD
  • PerkinElmer

    TAMRA-ddCTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • R6G-ddCTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • TSA Cyanine 3/Fluorescein System

    TSA Plus Cyanine 3 and Fluorescein System

    TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
    List Price : 708.00 USD
  • PerkinElmer

    ROX-ddUTP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 474.00 USD
  • PerkinElmer

    Cyanine 3-ddATP

    Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
    List Price : 567.00 USD
  • PerkinElmer

    DNP-11-UTP

    Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
    List Price : 347.00 USD
  • TSA Plus Cyanine 3.5

    TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
    Price Range : 225.00 USD - 1797.00 USD
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