PerkinElmer

Immunohistochemistry

OF

For more reliable results and enhanced sensitivity in chromogenic and fluorescent methods, our TSA reagents can be used in any immunohistochemistry (IHC) assay. They use much less primary antibody, which improves specificity and reduces background. Other benefits of this method include:

  • Improved limit of detection and quantitative range
  • Excellent localization of signal to the target
  • Enhanced fluorescent signal stability
  • Reagent kits that enable multiplexed immunohistochemistry for up to 6 targets at once. The workflow associated with these kits is similar to standard methods and accessible to any lab currently working with immunohistochemistry
  • Use the best unlabeled primary antibodies together in multiplex panels, with no species based crosstalk
  • Confirm single cell co-expression for many biomarkers in one tissue section
  • Get more information while conserving precious tissue

Tumor Microenvironment Opal 7 Color Image
Tumor Microenvironment Opal 7 Color Image

Stain

Simultaneously detect six biomarkers plus DAPI within a single tissue section. Similar to standard immunohistochemistry, our advanced method is accessible to many laboratories where IHC method development is performed. It allows researchers to use the best primary antibodies, even those raised in the same species, together for multiplex detection. Our staining solutions provide researchers with tools to assess multiple cellular phenotypes at the same time while retaining context provided within tissue sections.
Vectra® Polaris™ Automated Quantitative Pathology Imaging System
Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Image

Our quantitative pathology imaging systems deliver high quality data where morphological context is preserved down to the sub-cellular level, removing autofluorescence and increasing signal-to-noise. These systems detect and measure multiple biomarkers, even if overlapping, within a single IHC or IF tissue section or TMA. Low signal levels that were previously undetected can now be captured, and overall quantification is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
inform Image Analysis Software for Digital Pathology
inform Image Analysis Software for Digital Pathology

Analyze

Our software programs combine the latest technologies with algorithms and intuitive, easy-to-use interfaces, giving you the power to make new, exciting discoveries from your data. Our automated advanced image analysis software allows you to visualize, analyze, quantify and phenotype cells labeled with multiple biomarkers in situ in FFPE tissue sections.
Contract Research Services for Phenotyping In Situ
Contract Research Services for Phenotyping In Situ

Contract Research Services

Interested in testing the Phenoptics™ workflow? Our Research Services team can perform multiplexed IHC for up to seven colors (six markers plus DAPI) to help you get results.
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Application
  • Immunohistochemistry (IHC) (6)
  • In situ hybridization (ISH) (2)
Assay Target
  • CD20 (3)
  • CD4 (4)
  • CD45RO (1)
  • CD68 (2)
  • CD8 (4)
  • FOXP3 (2)
  • panCK (2)
Automation Compatible
  • No (4)
Detection Method
  • Fluorescence (4)
  • MSI (3)
Fluorophore
  • Cy3.5 (1)
  • Cy5.5 (1)
  • Opal 520 (4)
  • Opal 540 (3)
  • Opal 570 (4)
  • Opal 620 (3)
  • Opal 650 (3)
  • Opal 670 (1)
  • Opal 690 (3)
Format
  • Slides (2)
Imaging Modality
  • Multispectral Imaging (4)
Product Brand Name
  • Ancillary Products (1)
  • Opal (5)
  • TSA Plus (2)
Target Species
  • Human (4)
Type
  • Opal 520 (1)
  • Opal 540 (1)
  • Opal 570 (1)
  • Opal 620 (1)
  • Opal 650 (1)
  • Opal 690 (1)
Volume
  • 0-50 (1)

"Immunohistochemistry" 1-10 of 10 Products and Services

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Opal 520 Reagent Pack

Opal Reagent Packs

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 118.00 USD - 134.00 USD
Opal 4 Lymphocyte Kit, 50 slides

Opal 4 Lymphocyte Panel Kit

The Opal™ 4 Tumor Lymphocyte Kit enables phenotyping of helper T cells, cytotoxic T cells and B cells within intact FFPE tissue sections.
List Price : 1646.00 USD
PerkinElmer

TSA Blocking Reagent

TSA Blocking Reagent is used to make TNB Blocking buffer for use in in situ hybridization (ISH), immunofluorescence (IF), and immunohistochemistry (IHC) procedures. TNB Blocking Buffer is employed for preliminary blocking steps and also as a diluent for antibodies and peroxidase conjugates.
Price Range : 158.00 USD - 498.00 USD
Opal 7 Immunology Discovery Kit

Opal 7 Immunology Discovery Panel Kit

The Opal™ 7 Immunology Discovery Kit enables phenotyping of T cells, B cells and macrophages, with 3 open channels for user-selected targets.
List Price : 2758.00 USD
Opal 4-Color Automation IHC Kit 50 slides - NEL800001KT

Opal Automation IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 856.00 USD - 1132.00 USD
Opal 7 Tumor Infiltrating Lymphocyte Kit

Opal 7 Tumor Infiltrating Lymphocyte Kit

The Opal Tumor Infiltrating Lymphocyte Kit enables phenotyping of T cells, B cells and cancer cells within intact FFPE tissue sections.
List Price : 3492.00 USD
PerkinElmer

TSA Plus Cyanine 5.5

TSA® Plus Cyanine 5.5 (Cy5.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5.5 in the localized area of the probe or antibody.
Price Range : 240.00 USD - 1919.00 USD

TSA Plus Cyanine 3.5

TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
Price Range : 240.00 USD - 1919.00 USD
Opal 7 Solid Tumor Immunology Kit, 25 slides

Opal 7 Solid Tumor Immunology Kit

The Opal™ 7 Solid Tumor Immunology Kit enables phenotyping of helper T cells, cytotoxic T cells, B cells, macrophages and cancer cells within intact FFPE tissue sections.
List Price : 3492.00 USD
Tissue image

Opal Multiplex Manual IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 952.00 USD - 1204.00 USD

"Immunohistochemistry" 1-10 of 10 Products and Services