PerkinElmer

Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

  • Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
  • TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
  • Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Radioisotope
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  • Eu-N1 (5)
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Assay points(200-50,000)
  • 100 (41)
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  • 50000 (94)
Assay Target Class
  • Histone (10)
  • Phosphoprotein (110)
  • Protein (24)
Automation Compatible
  • Yes (233)
Experimental Type
  • In vitro (163)
Format
  • Microplates (177)
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"Enzymology" 1-50 of 289 Products & Services

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LabChip EZ Reader

EZ READER, SHIP LEVEL

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.

SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1687.00 USD - 18200.00 USD

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 306.00 USD - 6300.00 USD
AlphaLISA SureFire Ultra kits

Phospho-Ribosomal Protein S6 (Ser240/244) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
Price Range : 504.00 USD - 29200.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2481) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (phosphorylated on Ser2481) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1687.00 USD - 18200.00 USD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 327.00 USD - 890.00 USD

Anti-methyl-Histone H4 Arginine 3 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H4 methylated at arginine 3 (H4R3me). This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1481.00 USD - 27000.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 34, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 34 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 757.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated Histone H3 Lysine 9 (H3K9ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 9 (H3K9ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 725.00 USD - 12900.00 USD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit

The AlphaScreen® SureFire® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1687.00 USD - 18200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 504.00 USD - 29200.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 21, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 21 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 757.00 USD

LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 224.00 USD - 10200.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3632.00 USD - 9100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD

Anti-Histone H3 (C-term) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L. Use in conjunction with biotinylated anti-H3K79me2 (AL148).
Price Range : 1481.00 USD - 27000.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 504.00 USD - 29200.00 USD
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 5, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1637.00 USD

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 1154.00 USD - 4087.00 USD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
SureFire principle

AlphaScreen SureFire EIF4E (p-Ser209) Assay Kit

The AlphaScreen® SureFire® p-eIF4E (Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
AlphaLISA SureFire Ultra kits

Total ZAP-70 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ total ZAP-70 assay is a sandwich immunoassay for quantitative detection of total ZAP-70 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-ZAP-70.
Price Range : 504.00 USD - 30400.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 7, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1637.00 USD

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1541.00 USD - 28000.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD1 (Ser463/465) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD1 (Ser463/465) kits are designed for the detection of SMAD1 (phosphorylated at S463/465) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-EGFR (Tyr1068) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-EGFR (Tyr1068) kits are designed for the detection of EGFR (phosphorylated at residue Y1068) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD

AlphaLISA Anti-unmodified Histone H3 Lysine 4 (H3K4) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 unmodified against lysine 4 (H3K4). These beads can be used for no-wash AlphaLISA epigenetic eraser assays.
Price Range : 1481.00 USD - 27000.00 USD
PerkinElmer

PDE Substrate iFL-cAMP, for LabChip EZ Reader

Fluorescently-labelled cAMP for Phosphodiesterase Assays (Enyzme inhibiton, target discovery, lead optimization)
List Price : 757.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1538.00 USD - 16600.00 USD

AlphaLISA Anti-O-linked GlcNAc Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.
Price Range : 1481.00 USD - 27000.00 USD

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: CSERRPHFPQFSYSASGTAR
Price Range : 306.00 USD - 1250.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 29200.00 USD
AlphaLISA SureFire Ultra kits

Phospho-Src (Tyr419) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-Src assay is a sandwich immunoassay for quantitative detection of Src (phosphorylated on Tyr419) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 30400.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 6, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1637.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2)

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3632.00 USD - 9100.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 33, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 33 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 757.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total STAT3 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total STAT3 kits are designed for the detection of total STAT3 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-STAT3, and is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
AlphaLISA SureFire Ultra kits

Phospho-MKK4 (Ser257) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-MKK4 assay is a sandwich immunoassay for quantitative detection of MKK4 (phosphorylated on Ser257) in cellular lysates using Alpha Technology.
Price Range : 504.00 USD - 30400.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total EGFR LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total EGFR kits are designed for the detection of EGFR (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-EGFR, and is compatible with both adherent and suspension cells.
Price Range : 1512.00 USD - 6800.00 USD
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1703.00 USD - 18200.00 USD
PerkinElmer

LabChip Sipper Chip Coating Reagent 8 (2 ml), for EZ Reader

Coating reagent 8 (CR8) is a positively-charged additive that can be added to the separation buffer. It will bind the silanol groups of the glass channels in the LabChip multi-sipper chip (the glass is negatively-charged) to neutralize the charge.
List Price : 146.00 USD

"Enzymology" 1-50 of 289 Products & Services

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