PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLisa Analyte 2

LAG-3 (human), lyophilized (AlphaLISA)

Human LAG-3 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human LAG-3 Detection Kit, but can also be ordered separately.
List Price : 421.00 USD
AlphaLISA immunoassay kits

FSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FSH enables the quantitative determination of human follicle stimulating hormone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 541.00 USD - 16200.00 USD
AlphaLISA immunoassay kits

IL-17A (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 726.00 USD - 20400.00 USD
AlphaLISA immunoassay kits

BACE-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 451.00 USD - 9900.00 USD

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 2130.00 USD - 7400.00 USD
AlphaLisa Analyte 2

HLA-C (human), liquid (AlphaLISA)

Human HLA-C standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human HLA-C Detection Kit, but can also be ordered separately.
List Price : 421.00 USD
AlphaLisa Analyte 2

IFN-γ (porcine), lyophilized (AlphaLISA)

Porcine IFN-γ standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA porcine IFN-γ Detection Kit, but can be ordered separately.
List Price : 323.00 USD
AlphaLisa Analyte 2

Cry1F analyte, lyophilized (AlphaLISA)

Cry1F standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Cry1F kit, but can be ordered separately.
List Price : 344.00 USD
LANCE Detection Kit

FSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 806.00 USD - 10700.00 USD

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 644.00 USD - 19300.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL17 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 374.00 USD - 10500.00 USD
AlphaLISA immunoassay kits

PDGF (BB dimer) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 15000.00 USD
AlphaLISA immunoassay kits

GM-CSF (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Detection Kit is designed for detection and quantitation of mouse GM-CSF in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3036.00 USD - 23700.00 USD

Anti-methyl-Histone H3 Lysine 9 (H3K9me2) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 9 (H3K9me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1481.00 USD - 27000.00 USD
AlphaLisa Analyte 2

PON-1 (human), lyophilized (AlphaLISA)

Human paraoxonase/arylesterase 1 (PON-1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human PON-1 Detection Kit, but can also be ordered separately.
List Price : 782.00 USD
AlphaLISA Buffer

AlphaLISA Lysis Buffer

Pre-formulated buffer that can be used as a general lysis buffer for Alpha assays.
Price Range : 144.00 USD - 748.00 USD
AlphaLISA immunoassay kits

TNFR1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of human TNFR1 in buffered solution, serum, lysates, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 11100.00 USD
AlphaLISA immunoassay kits

IL-8 (bovine/ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.
Price Range : 724.00 USD - 10500.00 USD
Protein

Nidogen 1 (human), lyophilized (LANCE)

Human Nidogen 1 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra Nidogen 1 TR-FRET Detection Kit, but can be ordered separately.
List Price : 317.00 USD
AlphaLisa Analyte 2

Oncostatin M (human), lyophilized (AlphaLISA)

Human Oncostatin M standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Oncostatin M Detection Kit, but can be ordered separately.
List Price : 257.00 USD

DELFIA Assay Buffer

DELFIA® Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA time-resolved fluorescence (TRF) assays.
Price Range : 40.00 USD - 722.00 USD

GAD67 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.
Price Range : 805.00 USD - 25800.00 USD
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 502.00 USD - 9100.00 USD
AlphaLISA immunoassay kits

IL-8 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1109.00 USD - 10700.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human ICAM-1 Detection Kit

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 672.00 USD - 23700.00 USD

Anti-DIG AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a mouse monoclonal anti-Dig (digoxin) antibody. These beads can be used to capture Dig-labeled proteins, peptides, and other biomolecules to create AlphaLISA no-wash assays.
Price Range : 613.00 USD - 25200.00 USD

IL-31 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-31 Detection Kit is designed for detection and quantitation of human interleukin-31 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 806.00 USD - 10700.00 USD
Assay principle for LANCE TR-FRET binding assay

CTLA4-CD86 AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human CTLA-4 and CD86, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding to CTLA-4/CD86.
Price Range : 1876.00 USD - 11400.00 USD
AlphaLisa Analyte 2

N-cadherin (human), lyophilized (AlphaLISA)

Human N-cadherin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human N-cadherin Detection Kit, but can be ordered separately.
List Price : 258.00 USD
AlphaLISA immunoassay kits

Progesterone AlphaLISA Detection Kit

The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 724.00 USD - 10500.00 USD
AlphaLISA immunoassay kits

C-peptide (human) AlphaLISA Kit

AlphaLISA® no-wash immunoassay kit for detection of human C-peptide (human connecting peptide) in serum, buffered solution or cell culture medium.
Price Range : 1539.00 USD - 10200.00 USD
AlphaLISA immunoassay kits

Amyloid β 1-42 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 3036.00 USD - 23700.00 USD

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1541.00 USD - 28000.00 USD
AlphaLISA immunoassay kits

B7-H5/VISTA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human V-domain Ig suppressor of T cell activation (VISTA) enables the quantitative determination of human VISTA (B7-H5, PD-1H) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 15000.00 USD

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 589.00 USD - 24200.00 USD

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3632.00 USD - 9100.00 USD
AlphaLISA immunoassay kits

LAG-3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human LAG-3 enables the quantitative determination of human lymphocyte-activation gene 3 (LAG-3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 15000.00 USD

Anti-IgG4 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG4. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG4 in different sample matrices, including monkey serum.
Price Range : 594.00 USD - 24400.00 USD
AlphaLISA Cell-Histone Buffer Set for epigenetic assays, 500

AlphaLISA Cell-Histone Buffer Set

Pre-formulated buffer for cell-based Alpha epigenetic assays.
Price Range : 889.00 USD - 7000.00 USD
AlphaLisa Analyte 2

TNFR1 (human), lyophilized (AlphaLISA)

Human tumor necrosis factor receptor 1 (TNFR1) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human TNFR1 detection kit, but can also be ordered separately.
List Price : 421.00 USD
AlphaLISA immunoassay kits

IL-21 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-21 enables the quantitative determination of human interleukin-12 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 15000.00 USD

IFN-γ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
Price Range : 649.00 USD - 21100.00 USD
AlphaLISA immunoassay kits

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 725.00 USD - 10200.00 USD
Protein

COL1A1 (human), lyophilized (LANCE)

Human COL1A1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COL1A1 TR-FRET Detection Kit, but can also be ordered separately.
List Price : 308.00 USD
AlphaLISA immunoassay kits

EGF (human) AlphaLISA Detection Kit

The AlphaLISA® EGF Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor (EGF) in cell culture media, serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 672.00 USD - 10500.00 USD

DELFIA® Enhancement Solution, 250 mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 236.00 USD

CXCL4/PF4 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
Price Range : 644.00 USD - 19300.00 USD
AlphaLISA immunoassay kits

IL-12 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-12 enables the quantitative determination of bovine IL-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 18800.00 USD
PerkinElmer

LANCE Ultra hIL-11,lyophilized(0.3ug)

LANCE Ultra hIL-11,lyophilized(0.3ug)
List Price : 250.00 USD

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