PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA immunoassay kits

Alpha-1 Anti-Trypsin (A1AT) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human A1AT enables the detection and quantitation of human A1AT (hA1AT) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with Human Anti-thrombin and mouse A1AT.
Price Range : 626.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 2912.00 USD - 22800.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA immunoassay kits

MMP-3 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Matrix Metalloproteinase-3 (MMP3) Detection Kit is designed for detection and quantitation of human MMP3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP3 and pro-MMP3.
Price Range : 2912.00 USD - 22800.00 USD
PerkinElmer

FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 583.00 USD - 10600.00 USD
LANCE Detection Kit

IL-23A (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-23A Detection Kit is designed for detection and quantitation of human interleukin-23A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 773.00 USD - 10300.00 USD
AlphaLisa Analyte 2

Myeloperoxidase (human), lyophilized (AlphaLISA)

Human Myeloperoxidase (MPO) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human MPO kit, but can be ordered separately.
List Price : 529.00 USD

LANCE Ultra cAMP Detection Kit

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
Price Range : 637.00 USD - 8300.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA assay principle

Cardiac Troponin T (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 618.00 USD - 18500.00 USD
Protein

Lambda Light Chain (human), lyophilized (LANCE)

Human lambda light chain standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human Lambda Light Chain Detection Kit, but can also be ordered separately.
List Price : 240.00 USD

AlphaScreen C-MYC Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-c-myc-conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 180.00 USD - 13800.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 1653.00 USD - 17700.00 USD

LANCE® Eu-W1024 Anti-Rabbit IgG

Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 1043.00 USD - 9100.00 USD

LANCE Ultra ULight™-PKC substrate

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.
Price Range : 400.00 USD - 1089.00 USD
SureFire principle

AlphaScreen SureFire SMAD1 (p-Ser463;Ser465) Assay Kit

The AlphaScreen® SureFire® p-SMAD1 (p-Ser463/465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/465) in cellular lysates using Alpha Technology.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA immunoassay kits

PON-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 728.00 USD - 14000.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IP10 Detection Kit

The AlphaPlex Samarium (Sm) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 688.00 USD - 10000.00 USD
AlphaLISA assay principle

IL-31 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL-31 enables the quantitative determination of human IL-31 in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse IL31. Cross-reactivity with other species has not been tested.
Price Range : 618.00 USD - 18500.00 USD

LANCE Ultra KinaSelect™ Ser/Thr Kit, 5 X 250 assay points

LANCE® Ultra TR-FRET Ser/Thr kinase substrate selection kit, containing five different ULight-peptide substrates and corresponding Europium anti-phospho antibodies for kinase reactions.
List Price : 1902.00 USD
AlphaLISA immunoassay kits

IL-2 (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 695.00 USD - 10100.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 IL-6 Detection Kit 

The AlphaPlex™ 545 (Terbium) IL-6 Detection Kit is designed for the quantitative determination of human IL6 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 607.00 USD - 10000.00 USD
AlphaLISA immunoassay kits

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

GAD67 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.
Price Range : 772.00 USD - 24700.00 USD
alphascreen surefire total

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 409.00 USD - 16100.00 USD

Anti-IgG1 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG1. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG1 in different sample matrices, including monkey serum.
Price Range : 570.00 USD - 23400.00 USD
LANCE Detection Kit

IgG (bovine) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Bovine IgG Detection Kit is designed for detection and quantitation of bovine IgG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 1433.00 USD - 9500.00 USD

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 227.00 USD - 13800.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology.
Price Range : 1493.00 USD - 16100.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL1β Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL1β Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 588.00 USD - 10000.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-Mouse IgG

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 1043.00 USD - 9100.00 USD
AlphaLISA immunoassay kits

TNFα (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 645.00 USD - 10300.00 USD
AlphaLISA immunoassay kits

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
Price Range : 695.00 USD - 9700.00 USD

LANCE Ultra ULight™-anti-GST

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.
Price Range : 471.00 USD - 11200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Ser1106) in cellular lysates using Alpha Technology.
Price Range : 1493.00 USD - 16100.00 USD
Protein

Fibrinogen (human), lyophilized (LANCE)

Human fibrinogen standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human fibrinogen TR-FRET Detection Kit, but can also be ordered separately.
List Price : 295.00 USD
LANCE Detection Kit

p53 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra p53 Detection Kit is designed for detection and quantitation of human p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 2240.00 USD - 10300.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1532.00 USD - 7100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Protein

IL-23 (human), lyophilized (AlphaLISA)

Human IL-23 standard, for use in running standard curves in AlphaLISA assays.
List Price : 304.00 USD
AlphaLISA immunoassay kits

TAU (human) AlphaLISA Detection Kit

The AlphaLISA® Human Microtubule-associated Protein Tau (TAU) Detection Kit is designed for detection and quantitation of human TAU in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies used in the kit correspond to clone numbers BT2 and Tau 12.
Price Range : 2912.00 USD - 22800.00 USD

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 3484.00 USD - 8700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA immunoassay kits

MMP-2 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Matrix Metalloproteinase-2 (MMP2) Detection Kit is designed for detection and quantitation of human MMP2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP2 and pro-MMP2.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

EGFR (human) AlphaLISA Detection Kit

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 695.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

Collagen IV (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human collagen IV enables the quantitative determination of collagen IV in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLisa Analyte 2

Biotinylated Human FCGR3A (176Val), lyophilized (AlphaLISA)

Biotinylated human FCGR3A (176Val) receptor. For use in AlphaLISA® no-wash assays.
List Price : 265.00 USD
AlphaLISA immunoassay kits

CXCL10/IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-X-C Motif Chemokine 10 / 10 kDa Interferon- gamma-Induced Protein (CXCL10 / IP-10) Detection Kit is designed for detection and quantitation of human CXCL10/IP-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

IL-28B (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-28B enables the detection and quantitation of human Interleukin-28B in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL28A, IL29 or Mouse IL28B. Cross reactivity with other species has not been tested.
Price Range : 446.00 USD - 9700.00 USD

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