PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA immunoassay kits

Lambda light chain (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lambda light chain enables the quantitative determination of human λ light chain in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 15000.00 USD
AlphaLISA immunoassay kits

α-tubulin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for α-tubulin enables the detection and quantitation of human α-tubulin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 726.00 USD - 20400.00 USD
AlphaLISA immunoassay kits

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.
Price Range : 1585.00 USD - 10500.00 USD
AlphaLisa Analyte 2

BCL-2 (human), lyophilized (AlphaLISA)

Human BCL-2 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA BCL-2 Detection Kit, but can be ordered separately.
List Price : 322.00 USD
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 644.00 USD - 19300.00 USD
AlphaLISA immunoassay kits

p62 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for p-62 enables the detection and quantitation of human p62 in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 598.00 USD - 13100.00 USD
AlphaLISA assay principle

ANGPT2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPT2 enables the quantitative determination of human Angiopoietin 2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 18800.00 USD

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 451.00 USD - 9900.00 USD
AlphaLISA immunoassay kits

IL-6 (human) AlphaLISA Biotin-free Detection Kit

The AlphaLISA Human IL-6 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-6 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 451.00 USD - 9900.00 USD
AlphaLISA immunoassay kits

IgA (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 646.00 USD - 10100.00 USD
AlphaLisa Analyte 2

EGFR (human), lyophilized (AlphaLISA)

EGFR (human), for use in the AlphaLISA EGF/EGFR Binding Assay.
List Price : 250.00 USD
LANCE Detection Kit

ApoE (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ApoE Detection Kit is designed for detection and quantitation of human Apolipoprotein E (ApoE) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 806.00 USD - 10700.00 USD
AlphaLISA immunoassay kits

Chloramphenicol AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 446.00 USD - 10500.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT3 (Tyr705) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT3 (Tyr705) in a multiplexed assay.
Price Range : 378.00 USD - 22800.00 USD
AlphaLISA SureFire Ultra kits

Total VEGFR-2 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ Total VEGFR-2 assay is a sandwich immunoassay for quantitative detection of VEGF receptor 2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 489.00 USD - 29500.00 USD

CCL5/RANTES (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3036.00 USD - 23700.00 USD

Anti-Mouse IgA AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgA. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 589.00 USD - 24200.00 USD
AlphaLISA Binding Assay kits

PD-1/PD-L1 (human) AlphaLISA Binding Kit

The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.
Price Range : 725.00 USD - 10200.00 USD
AlphaLisa Analyte 2

Apolipoprotein E (human), lyophilized (AlphaLISA)

Human ApoE standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA ApoE Detection Kit, but can be ordered separately.
List Price : 317.00 USD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 327.00 USD - 890.00 USD
PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
Price Range : 310.00 USD - 14900.00 USD

IFN-λ2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human interferon lambda-2 (IFN-λ2) enables the quantitative determination of human IFN-λ2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 451.00 USD - 9900.00 USD
AlphaLISA immunoassay kits

ANGPTL4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 451.00 USD - 9900.00 USD
AlphaLisa Analyte 2

IgE (mouse), lyophilized (AlphaLISA)

Mouse IgE standard, for running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgE isotyping kit, but can be ordered separately.
List Price : 248.00 USD
AlphaLISA immunoassay kits

N-cadherin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human N-cadherin enables the detection and quantitation of human N-Cadherin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 478.00 USD - 10500.00 USD
AlphaLISA immunoassay kits

CD40 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 11100.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
Price Range : 1538.00 USD - 16600.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IFN-γ Detection Kit

The AlphaPlex™ 645 (Samarium) Human Interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 480.00 USD - 10500.00 USD
AlphaLisa Analyte 2

IgG2b (mouse), lyophilized (AlphaLISA)

Mouse IgG2b standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG2b kit, but can be ordered separately.
List Price : 248.00 USD
AlphaPlex Samarium Acceptor Beads

Protein A AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
Price Range : 612.00 USD - 25100.00 USD

ANGPTL3 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL3 Detection Kit is designed for detection and quantitation of human angiopoietin-like 3 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 830.00 USD - 11100.00 USD
AlphaLISA immunoassay kits

Utrophin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human utrophin enables the quantitative determination of utrophin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 301.00 USD - 18800.00 USD
AlphaLISA immunoassay kits

PD-L1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human PD-L1 Detection Kit is designed for detection and quantitation of Human PD-L1 (CD274/B7-H1) in immunoassay buffer, human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 725.00 USD - 10200.00 USD
AlphaLISA immunoassay kits

TNFR1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of human TNFR1 in buffered solution, serum, lysates, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 782.00 USD - 11100.00 USD
AlphaLISA immunoassay kits

FSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FSH enables the quantitative determination of human follicle stimulating hormone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 541.00 USD - 16200.00 USD

Nickel Chelate AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to nickel chelate. These beads can be used to capture His-tagged proteins and peptides.
Price Range : 613.00 USD - 25200.00 USD
PerkinElmer

LANCE Eu-anti-C-tag antibody

Europium-labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE ULight or SureLight™ APC-labeled reagents to create LANCE TR-FRET assays.
Price Range : 332.00 USD - 9400.00 USD

LANCE Ultra Europium-anti-phospho-40S Ribosomal Protein S6 (Ser235/236)

Europium-labeled rabbit monoclonal antibody recognizing human 40S ribosomal protein S6 phosphorylated at both Ser235 and Ser236. Sequence motif recognized: RRL(pS)(pS)LRA
Price Range : 745.00 USD - 2580.00 USD
AlphaLISA immunoassay kits

PDGF (BB dimer) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 625.00 USD - 15000.00 USD
AlphaLISA immunoassay kits

hCG (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human chorionic gonadotropin (hCG) enables the quantitative determination of hCG in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 541.00 USD - 16200.00 USD

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
Price Range : 725.00 USD - 10100.00 USD

IL-28B (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-28B enables the detection and quantitation of human Interleukin-28B in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL28A, IL29 or Mouse IL28B. Cross reactivity with other species has not been tested.
Price Range : 465.00 USD - 10200.00 USD
AlphaLISA immunoassay kits

IL-2 (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 725.00 USD - 10500.00 USD
Protein

MMP-12 (human), lyophilized (LANCE)

Human MMP-12 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra MMP-12 TR-FRET Detection Kit, but can be ordered separately.
List Price : 317.00 USD
AlphaLisa Analyte 2

Periostin (human), lyophilized (AlphaLISA)

Human periostin standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human periostin detection kit, but can also be ordered separately.
List Price : 759.00 USD
AlphaLISA immunoassay kits

TDP-43 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for TDP-43 is designed for the quantitative determination of Transactive response DNA binding protein 43 (TDP43) in human serum, CSF, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 649.00 USD - 21100.00 USD

Albumin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Albumin Detection Kit is designed for detection and quantitation of human albumin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 829.00 USD - 11100.00 USD
PerkinElmer

SF UltraPlexTb CaptSure Acc Beads

CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
Price Range : 287.00 USD - 11800.00 USD

Anti-IgG1 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG1. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG1 in different sample matrices, including monkey serum.
Price Range : 594.00 USD - 24400.00 USD

IL-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of mouse IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1585.00 USD - 10500.00 USD

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