PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

 

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE® Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

 

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA immunoassay kits

GDNF (human) AlphaLISA Detection kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human GDNF (glial cell-derived neurotrophic factor) in serum, plasma, cell culture supernatants, and other samples.
Price Range : 626.00 USD - 9700.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-6X His AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
AlphaLisa Analyte 2

VCAM-1 (human), lyophilized (AlphaLISA)

Human VCAM-1 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA VCAM-1 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLisa Analyte 2

p24 (human HIV), lyophilized (AlphaLISA)

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) standard, lyophilized, for use in running standard curves in AlphaLISA® no-wash immunoassays. This standard is already provided in the AlphaLISA HIV p24 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLisa Analyte 2

Human IgG, lyophilized (AlphaLISA)

Human IgG, lyophilized, 3 µg. Human IgG is included in the AlphaLISA® human IgG kits, but can also be ordered separately as a standalone item. One vial contains an amount of human IgG sufficient for performing 10 standard curves.
List Price : 240.00 USD
AlphaLISA immunoassay kits

COL1A1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human COL1A1 enables the detection and quantitation of human COL1A1 in serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD

MANF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 750.00 USD - 10000.00 USD
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 433.00 USD - 9500.00 USD
LANCE_Fc_receptor

FCGR3A/CD16a (176Val/V158) LANCE Ultra TR-FRET Binding Kit

This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Val/V158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
Price Range : 750.00 USD - 10000.00 USD

LANCE® Eu-W1024 labeled anti-GST antibody

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
Price Range : 205.00 USD - 10100.00 USD

IL-5 (human) Research kits

The AlphaLISA® Human Interleukin 5 (IL5) Detection Kit is designed for detection and quantitation of human IL5 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD

IL-23A (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-23A Detection Kit is designed for detection and quantitation of human interleukin-23A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 750.00 USD - 10000.00 USD

PSMA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PSMA enables the quantitative determination of human prostate specific membrane antigen (PSMA) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 14000.00 USD
AlphaLISA assay principle

MANF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MANF enables the quantitative determination of mesencephalic astrocyte-derived neurotrophic factor (MANF) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD

IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin 17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 773.00 USD - 10300.00 USD
AlphaLisa Analyte 2

GDNF (human), lyophilized (AlphaLISA)

Human GDNF (glial cell-derived neurotrophic factor) standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA GDNF detection kits, but can also be ordered separately.
List Price : 240.00 USD

Anti-Mouse IgE (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgE. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD
LANCE-bromodomain-binding

LANCE Ultra BRD4 (BD1)/H4K5,8,12,16Ac Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain1: BRD4 (BD1) and acetylated histone H4 peptide (H4 K5,8,12,16Ac) using a homogeneous LANCE Ultra TR-FRET assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 750.00 USD - 10000.00 USD
AlphaLisa Analyte 2

IL-4 (bovine), lyophilized (AlphaLISA)

Bovine IL-4 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-4 Detection Kit, but can be ordered separately.
List Price : 233.00 USD
Protein

LC3B (human), lyophilized (LANCE)

Human LC3B standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human LC3B TR-FRET Detection Kit, but can be ordered separately.
List Price : 236.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human TNFα Detection Kit

The AlphaPlex™ 545 (Terbium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 589.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 675.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

E-Cadherin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human E-cadherin enables the detection and quantitation of human E-cadherin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
PerkinElmer

AlphaLISA SureFire Ultra SMAD3 pS423/425 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Smad3 (Ser423/425) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Smad3 assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD

DELFIA®; Eu-N1 Goat anti-Rabbit IgG

Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
Price Range : 706.00 USD - 3206.00 USD
AlphaLISA immunoassay kits

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.
Price Range : 1476.00 USD - 9800.00 USD
AlphaPlex-545-antibody-bead2

Anti-unmodified H3K4 AlphaPlex-545 Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

Chloramphenicol AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 415.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

Hyaluronic acid (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.
Price Range : 675.00 USD - 9500.00 USD
AlphaLisa Analyte 2

MANF (human), lyophilized (LANCE)

Human MANF standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human MANF TR-FRET Detection Kit, but can also be ordered separately.
List Price : 226.00 USD

Insulin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 675.00 USD - 9500.00 USD

AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of Human Programmed Cell Death Protein 1 (PD-1) in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 626.00 USD - 9800.00 USD
PerkinElmer

LANCE Ultra m/rIL17A, lyophilized(1.0ug)

LANCE Ultra m/rIL17A, lyophilized(1.0ug)
List Price : 233.00 USD

BMP-7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BMP-7 enables the detection and quantitation of human BMP-7 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
List Price : 1605.00 USD

Monocyte Chemoattractant Protein-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) Detection Kit is designed for detection and quantitation of human CCL2/MCP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
Protein

Transferrin (human), lyophilized (LANCE)

Human transferrin standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Transferrin TR-FRET Detection Kit, but can be ordered separately.
List Price : 233.00 USD

AlphaLISA Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
alphascreen surefire total

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 397.00 USD - 15700.00 USD
AlphaPlex-545-antibody-bead2

Anti-6X His AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex-545 Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD

DELFIA® Eu-N1 Anti-GST

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in DELFIA® TRF assays.
Price Range : 257.00 USD - 3154.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 1488.00 USD - 6800.00 USD
AlphaLisa Analyte 2

MMP-8 (human), lyophilized (AlphaLISA)

Human MMP-8 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human MMP-8 Detection Kit, but can be ordered separately.
List Price : 233.00 USD
AlphaPlex-545-antibody-bead2

Anti-HA AlphaPlex 545 (Tb) Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-HA (hemaglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.
Price Range : 571.00 USD - 23400.00 USD
Alpha Fc receptor bind

FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 675.00 USD - 10300.00 USD
AlphaLISA immunoassay kits

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD

MMP-3 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MMP-3 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase-3 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 772.00 USD - 10300.00 USD
Protein

IL-31 (Human) lyophilized (AlphaLISA)

Human IL-31 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-31 Detection Kit, but can be ordered separately.
List Price : 295.00 USD
Protein

IL-17A (human), lyophilized (AlphaLISA)

Human IL-17A standard, for use in running standard curves in AlphaLISA assays.
List Price : 240.00 USD

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