PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

 

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE® Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

 

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
PerkinElmer

AlphaLISA SureFire Ultra CREB pS133 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-CREB (Ser133) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-CREB assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
alphascreen surefire total

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 397.00 USD - 15700.00 USD

LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
Price Range : 1984.00 USD - 6800.00 USD

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD

Anti-bovine IgM AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-bovine IgM antibody. These beads can be used to capture bovine IgM antibodies.
Price Range : 773.00 USD - 26100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Ser1106) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLisa Analyte 2

TNFα (bovine), lyophilized (AlphaLISA)

Bovine TNFα standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine TNFα kits, but can also be ordered separately.
List Price : 325.00 USD
AlphaLisa Analyte 2

IL-5 (human), lyophilized (AlphaLISA)

Human Interleukin 5 (IL5) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL5 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

Claudin 7 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps).
List Price : 2536.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 27600.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Thr1343) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD

IgG (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG (total) in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 675.00 USD - 10400.00 USD
AlphaLISA immunoassay kits

BCL-2 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
List Price : 2266.00 USD
AlphaLisa Analyte 2

Leptin (human), lyophilized (AlphaLISA)

Human Leptin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human leptin kit, but can be ordered separately.
List Price : 240.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
Price Range : 1399.00 USD - 4541.00 USD
AlphaLisa Analyte 2

IL-6 (human), lyophilized (AlphaLISA)

Human Interleukin-6 (IL6) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL-6 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

Heme oxygenase 2 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
PerkinElmer

AlphaLISA SureFire Ultra 4EBP1 pT37/46 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-4E-BP1 (Thr37/46) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-4E-BP1 assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total MEK1 Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 27600.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
alphascreen surefire total

AlphaScreen SureFire Total Akt1 research kit

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 1590.00 USD - 17200.00 USD
PerkinElmer

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 1075.00 USD - 3806.00 USD

DELFIA Anti-phospho-(Ser) 14-3-3 motif, 10µg

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for capturing antibodies in DELFIA® TRF kinase assays.
List Price : 2642.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-MEK1 (Ser218/222) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
List Price : 1605.00 USD
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 50,000 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.
List Price : 17200.00 USD

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 349.00 USD - 2794.00 USD
Protein

COL1A1 (human), lyophilized (LANCE)

Human COL1A1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COL1A1 TR-FRET Detection Kit, but can also be ordered separately.
List Price : 287.00 USD
SureFire principle

AlphaScreen SureFire SMAD1 (p-Ser463;Ser465) Assay Kit

The AlphaScreen® SureFire® p-SMAD1 (p-Ser463/465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/465) in cellular lysates using Alpha Technology.
Price Range : 1588.00 USD - 17200.00 USD
AlphaLISA immunoassay kits

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit, 100 Assay Points

The AlphaLISA® Human BDNF Biotin-Free Detection Kit is designed for the quantitative detection of human Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
List Price : 445.00 USD

CCL2/MCP-1α (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse CCL2/MCP-1α Detection Kit is designed for detection and quantitation of mouse Monocyte Chemoattractant Protein 1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 773.00 USD - 10300.00 USD

IgG (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgG in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 1419.00 USD - 9400.00 USD
Protein

MANF (human) lyophilized (AlphaLISA)

Human MANF standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human MANF Detection Kit, but can be ordered separately.
List Price : 295.00 USD
AlphaLisa Analyte 2

IL-7 (human), lyophilized (AlphaLISA)

Human Interleukin 7 (IL7) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL7 kit, but can be ordered separately.
List Price : 240.00 USD

IgG (rabbit) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Rabbit IgG Detection Kit is designed for detection and quantitation of rabbit IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 773.00 USD - 10300.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pY701 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
List Price : 475.00 USD

CXCL10/IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-X-C Motif Chemokine 10 / 10 kDa Interferon- gamma-Induced Protein (CXCL10 / IP-10) Detection Kit is designed for detection and quantitation of human CXCL10/IP-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
PerkinElmer

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
Price Range : 1488.00 USD - 6800.00 USD

DELFIA Eu-N1 labeled anti-phosphoserine antibody, 10µg

Europium-labeled anti-phosphoserine antibody, for capturing antibodies in DELFIA® TRF kinase assays.
List Price : 1239.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 10,000 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.
List Price : 7000.00 USD

BMP-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BMP-9 enables the quantitative determination of human Bone Morphogenetic Protein 9 (BMP-9) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH Assay Kit

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.
Price Range : 475.00 USD - 27500.00 USD

COL1A1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human COL1A1 Detection Kit is designed for detection and quantitation of human COL1A1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 750.00 USD - 10000.00 USD
PerkinElmer

AlphaLISA SureFire Ultra AKT 1/2/3 pT308 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Akt1/2/3 (Thr308) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Akt1/2/3 assay kit, but can also be ordered separately.
List Price : 21.00 USD
Protein

FGF23 (Human) lyophilized (AlphaLISA)

Human FGF23 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA FGF23 Detection Kit, but can be ordered separately.
List Price : 233.00 USD

MMP-12 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MMP-12 Detection Kit is designed for detection and quantitation of Matrix metalloproteinase 12 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 772.00 USD - 10300.00 USD
AlphaLisa Analyte 2

IgG (mouse), lyophilized (AlphaLISA)

Mouse IgG standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA mouse IgG kits, but can also be ordered separately.
List Price : 231.00 USD

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