DELFIA TRF

OF

When washing and separation are essential to avoid high sample-dependent background or dissociate sticky complexes, turn to our DELFIA® time-resolved fluorescence (TRF) technology for a solid-phase heterogeneous assay similar to ELISA -- but with larger dynamic range and higher signal-to-noise. DELFIA utilizes the unique chemical properties of lanthanide chelates in concert with time-resolved fluorescence (TRF) detection to create a wash-based assay that offers high sensitivity, wide dynamic range, superior stability, and excellent flexibility.

Advantages:

  • High sensitivity: femtogram-level detection
  • Low background: large Stokes' shift and time-resolved measurement
  • Wide dynamic range: 4-5 log
  • Superior stability: lanthanide chelates allow for delayed detection and batch processing
  • Excellent flexibility: easily converted from 96-well to 384-well format
  • Multiplexing capability

Applications:

  • Immunodetection and immunogenicity
  • Receptor-ligand binding
  • Enzymatic assays
  • Cell cytotoxicity and proliferation
  • Protein-protein interactions
  • Biodistribution
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    Antibody conjugates
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    • Anti-phospho-threonine (1)
    • Anti-rabbit IgG (1)
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    • Streptavidin (3)
    Application
    • Kinase (6)
    • Protein Analysis & Detection (29)
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    Molecular modification
    • Phosphorylation (6)

    "DELFIA TRF" 1-50 of 55 Products and Services

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    DELFIA® L*R binding buffer concentrate (10x), 250 mL

    Buffer for DELFIA® time-resolved fluorescence (TRF) ligand binding assays.
    List Price : 517.00 USD

    DELFIA® Eu-N1 Anti-c-myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in DELFIA® TRF assays.
    Price Range : 592.00 USD - 5600.00 USD

    DELFIA Eu-N1-ITC Chelate and Eu Standard, 20 mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
    List Price : 11100.00 USD

    DELFIA® Eu-N1 Anti-6xHis

    Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
    Price Range : 438.00 USD - 4089.00 USD

    DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

    Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
    Price Range : 243.00 USD - 3063.00 USD

    DELFIA Sm-N1-Iodoacetomido Chelate and Samarium (Sm) Standard, 1mg

    Samarium labeling chelate for creating your own Samarium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The Iodacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
    List Price : 2015.00 USD

    DELFIA Assay Buffer

    DELFIA® Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA time-resolved fluorescence (TRF) assays.
    Price Range : 36.00 USD - 653.00 USD

    DELFIA® Inducer, 250 mL

    DELFIA® Inducer is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This buffer is an alternative to Enhancement Solution for Eu and/or Sm measurements.
    List Price : 222.00 USD

    DELFIA Eu-N1-Iodoacetomido Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The Iodacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
    List Price : 2015.00 USD

    DELFIA Tb-N1-Iodoacetomido Chelate and Terbium (Tb) Standard, 1mg

    Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The Iodacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
    List Price : 2015.00 USD

    DELFIA Eu-N1 labeled anti-phosphoserine antibody, 10µg

    Europium-labeled anti-phosphoserine antibody, for capturing antibodies in DELFIA® TRF kinase assays.
    List Price : 1203.00 USD

    DELFIA® Eu-N1 Anti-HA

    Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
    Price Range : 592.00 USD - 5600.00 USD

    DELFIA®; Eu-N1 Goat anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
    Price Range : 685.00 USD - 3113.00 USD

    DELFIA® Wash Concentrate, 250mL

    Concentrated washing solution for DELFIA® time-resolved fluorescence (TRF) assays.
    List Price : 72.00 USD

    DELFIA Sm-Labeling kit (Sm-N1-ITC Chelate), 0.2mg

    The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.
    List Price : 510.00 USD

    DELFIA Eu-N1 rabbit anti-mouse-IgG

    Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA® TRF assays.
    Price Range : 262.00 USD - 1136.00 USD

    DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

    DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
    List Price : 510.00 USD

    DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

    Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
    Price Range : 227.00 USD - 3051.00 USD

    DELFIA® Assay Buffer without detergents, 5x conc.

    Detergent-free assay buffer for DELFIA® time-resolved fluorescence (TRF) assays.
    List Price : 803.00 USD

    DELFIA Anti-phospho-(Ser) 14-3-3 motif, 10µg

    Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for capturing antibodies in DELFIA® TRF kinase assays.
    List Price : 2565.00 USD

    DELFIA Eu-N1 ITC chelate & Eu standard, 1 mg

    DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
    List Price : 2015.00 USD

    DELFIA Tb-N1 Streptavidin

    Terbium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
    Price Range : 289.00 USD - 1971.00 USD

    DELFIA Sm-N1 Streptavidin

    Samarium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
    Price Range : 289.00 USD - 1971.00 USD

    DELFIA® Enhancement Solution, 50 mL

    DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
    List Price : 42.00 USD

    DELFIA® Eu-N1 Anti-GST

    Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in DELFIA® TRF assays.
    Price Range : 249.00 USD - 3063.00 USD

    DELFIA Eu-DTPA-ITC Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2015.00 USD

    Fluorescein Standard Solution, 50mL, 100nMole/L

    100 nmol/L Fluorescein standard solution, 50 mL Shipped on blue ice.
    List Price : 148.00 USD

    DELFIA Sm-N1-ITC Chelate and Samarium (Sm) Standard, 1mg

    Samarium labeling chelate for creating your own Samarium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
    List Price : 2015.00 USD

    DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

    Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
    Price Range : 208.00 USD - 3063.00 USD

    DELFIA 1 nmol/L Europium standard solution, 50 mL

    Europium standard solution for DELFIA time-resolved fluorescence (TRF) assays.
    List Price : 149.00 USD

    DELFIA Eu-N1 Streptavidin

    DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.
    List Price : 359.00 USD

    DELFIA® BATDA Labeling Reagent, 50 µL

    BATDA reagents for labeling cells in DELFIA time-resolved fluroescence (TRF) assays.
    List Price : 175.00 USD

    DELFIA Eu-DTPA-amino Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2015.00 USD

    DELFIA Eu-N1 Labeled Anti-Phosphothreonine Antibody, 10µg

    Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
    List Price : 1209.00 USD

    DELFIA® Enhancement Solution, 250 mL

    DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
    List Price : 213.00 USD

    DELFIA® Enhancement Solution, 1000 mL

    DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
    List Price : 798.00 USD

    DELFIA® Enhancer, 50mL

    DELFIA Enhancer solution for forming fluorescent Terbium and Dysprosium chelates in DELFIA time-resolved fluorescence (TRF) assays.
    List Price : 170.00 USD

    DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg

    Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
    List Price : 2015.00 USD

    DELFIA Eu-N1 Anti-Human IgG, 100µg

    DELFIA® Europium-labeled anti-human IgG, for use in DELFIA time-resolved fluorescence (TRF) immunoassays.
    List Price : 321.00 USD

    DELFIA Tb-N1-DTA Chelate and Terbium (Tb) Standard, 1mg

    Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
    List Price : 2015.00 USD

    DELFIA® 1 nmol/L Terbium standard solution, 50 mL

    For GLP or for general problem solving, lanthanide standard solutions are ready-to-use preparations of europium, samarium or terbium.
    List Price : 116.00 USD

    DELFIA Eu-N1-DTA Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
    List Price : 2015.00 USD

    DELFIA® Lysis Buffer, 30mL

    DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.
    List Price : 240.00 USD

    DELFIA® Eu-Solution, 200mL (for Cytotoxicity Detection Only)

    DELFIA® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.
    List Price : 161.00 USD

    DELFIA Eu-N1-amino Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups.
    List Price : 2015.00 USD

    DELFIA Tb-DTPA-ITC Chelate and Terbium (Tb) Standard, 1mg

    Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2015.00 USD

    DELFIA Sm-N1-DTA Chelate and Samarium (Sm) Standard, 1mg

    Samarium labeling chelate for creating your own Samarium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
    List Price : 2015.00 USD

    DELFIA® Research Buffer Set, 2x conc.

    Protein- and detergent-free assay buffer in separate bottles, for DELFIA® time-resolved fluorescence (TRF) assays.
    List Price : 329.00 USD

    DELFIA 10 nmol/L Samarium standard solution, 50 mL

    Samarium standard solution for DELFIA time-resolved fluorescence (TRF) assays.
    List Price : 118.00 USD

    DELFIA®Cell proliferation kit, 10 x 96 wells

    DELFIA® time-resolved fluorescence (TRF) cell proliferation kit for measuring cytotoxicity or proliferation in mammalian cells.
    List Price : 672.00 USD

    "DELFIA TRF" 1-50 of 55 Products and Services