BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. It deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.
4CN Plus substrate detects HRP and is ideal for western blotting applications. It produces dark purple precipitate in the prescence of HRP and is 10 times more sensitive than standard 4CN(4-chloro-1-napthol) formulations.
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