From receptor ligand binding to intracellular pathway analysis of phosphorylated proteins, breakthrough discoveries in cell signaling and cell signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles.
We enable a deeper understanding and more biologically relevant results with complete cell-based and biochemical cell signaling assays featuring innovative reagent technologies like: AlphaLISA® SureFire® Ultra™, AlphaLISA® SureFire® Ultra™ Multiplex, AlphaScreen® SureFire®, LANCE® Ultra TR-FRET, and DELFIA® TRF assays – not to mention our reporter gene assays and complete line of industry leading radiochemicals.
With our highly reliable, high-performance multimode plate readers, including the EnVision® and Ensight™ systems, and automated microscope image capture and analysis we provide access to a wide range of detection technologies for everything you need to bring about the next big breakthrough in cell signaling and pathway analysis.
Alpha SureFire® assays are no-wash cellular kinase assays. While they exist in different formats (AlphaScreen® SureFire, AlphaLISA® SureFire Ultra, and Alpha SureFire Ultra Multiplex), all use PerkinElmer’s exclusive bead-based technology in a sandwich immunoassay format for the detection of phosphorylated proteins in cells. Alpha SureFire assays are a quantitative alternative to Western Blotting, are automation-friendly, miniaturizable, and detect both endogenous and recombinant proteins.
AlphaScreen SureFire has a rich track record of small molecule screening successes:
AlphaLISA SureFire Ultra
The labeling of one of the antibodies with CaptSure™, a small peptide tag, is recognized by CaptSure-antibody-coated beads, providing significant improvement over the AlphaScreen SureFire format:
Alpha SureFire Ultra Multiplex
This format utilizes AlphaPlex™ technology to read multiple signals from the same well:
We offer the widest selection of proven GPCR target solutions - beyond the ligand GPCR event to the actual assessment of the cellular-activated signal transduction cascade. Enabling you to identify, validate, screen, and profile your leads is our priority.
LANCE Ultra TR-FRET
LANCE® Ultra TR-FRET Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.
LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer) based, homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.
DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.
DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.
Radiometric detection is a direct method that removes the need for target specific antibodies, reducing assay time & variables. A range of isotopes (3H, 14C, 33P, 35S) can be labeled to a variety of without altering biological function to study in the most natural state receptor ligand binding, kinases, G proteins, and other cell signaling pathways.
For high throughput screening, SPA (Scintillation Proximity Assay) technology provides a homogenous radiometric format for a multitude of assays.
An alternative format, SPA Imaging Beads are designed for detection on CCD based microplate imagers They offer additional benefits to SPA Scintillation Beads, including:
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The EnVision 2105 multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Tried and tested, the EnVision microplate reader gives you robust performance and reliable data for high throughput screening, time after time.
The EnVision 2105 model delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). In addition, the Alpha 775 laser module is designed for plant assays.