PerkinElmer

Cell Signaling and Pathway Analysis

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From receptor ligand binding to intracellular pathway analysis of phosphorylated proteins, breakthrough discoveries in cell signaling and cell signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles.

We enable a deeper understanding and more biologically relevant results with complete cell-based and biochemical cell signaling assays featuring innovative reagent technologies like: AlphaLISA® SureFire® Ultra, AlphaLISA® SureFire® Ultra Multiplex, AlphaScreen® SureFire®, LANCE® Ultra TR-FRET, and DELFIA® TRF assays – not to mention our reporter gene assays and complete line of industry leading radiochemicals.

With our highly reliable, high-performance multimode plate readers, including the EnVision® and Ensight systems, and automated microscope image capture and analysis we provide access to a wide range of detection technologies for everything you need to bring about the next big breakthrough in cell signaling and pathway analysis.

Cellular Kinase Assays
Cellular Kinase Assays

Cellular Kinase Assays

Alpha SureFire® assays are no-wash cellular kinase assays. While they exist in different formats (AlphaScreen® SureFire, AlphaLISA® SureFire Ultra, and Alpha SureFire Ultra Multiplex), all use PerkinElmer’s exclusive bead-based technology in a sandwich immunoassay format for the detection of phosphorylated proteins in cells. Alpha SureFire assays are a quantitative alternative to Western Blotting, are automation-friendly, miniaturizable, and detect both endogenous and recombinant proteins.

AlphaScreen SureFire

AlphaScreen SureFire has a rich track record of small molecule screening successes:

  • Uses protein A to capture one of the kit’s antibodies
  • 4-hour incubation protocol
  • Need to order the AlphaScreen protein A kit in addition to the AlphaScreen SureFire kit

AlphaLISA SureFire Ultra

The labeling of one of the antibodies with CaptSure, a small peptide tag, is recognized by CaptSure-antibody-coated beads, providing significant improvement over the AlphaScreen SureFire format:

  • Lower background signal, improved sensitivity, and dynamic range
  • No interference from extraneous antibodies; ideal for therapeutic antibody screening
  • AlphaLISA beads are optimal for hemoglobin-containing samples
  • Quicker assay: 2-hour incubation protocol
  • Beads are included in the kit

Alpha SureFire Ultra Multiplex

This format utilizes AlphaPlex technology to read multiple signals from the same well:

  • Improves data quality with internal controls (e.g., total protein) or gathers more data (multiplex phosphorylation events) from a single experiment, resulting in significant time and cost savings
  • Same features as the AlphaLISA SureFire Ultra, beads are included in the kit
GPCR Studies
GPCR Studies

GPCR Studies

We offer the widest selection of proven GPCR target solutions - beyond the ligand GPCR event to the actual assessment of the cellular-activated signal transduction cascade. Enabling you to identify, validate, screen, and profile your leads is our priority.

LANCE Ultra TR-FRET

LANCE® Ultra TR-FRET Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer) based, homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.

Radiometric Detection

Radiometric detection is a direct method that removes the need for target specific antibodies, reducing assay time & variables. A range of isotopes (3H, 14C, 33P, 35S) can be labeled to a variety of without altering biological function to study in the most natural state receptor ligand binding, kinases, G proteins, and other cell signaling pathways.

For high throughput screening, SPA (Scintillation Proximity Assay) technology provides a homogenous radiometric format for a multitude of assays.

  • No separation or washing steps saves time and generates reliable results.
  • A wide range of SPA bead coatings allows a variety of targets to be assayed.
  • A more convenient, cost-effective, and safer alternative to radioactive filter binding assay
  • Amenable to automation for increased throughput.
  • SPA beads can be used in tubes, 96-, 384- or 1536-well microplates and their signal measured on any scintillation counter.
  • Assay miniaturization is possible, reducing total assay volumes and concentrations per well for lower reagents costs and higher throughput.
  • SPA beads are suitable for use with 3H-, 125I-, 33P-, 35S-, and 14C-labeled ligands.
  • No requirement for protein modification.

An alternative format, SPA Imaging Beads are designed for detection on CCD based microplate imagers They offer additional benefits to SPA Scintillation Beads, including:

  • Red-shifted signal minimizes quenching with colored compounds to reduce the number of false negatives.
  • Format-free imaging of any plate density increases throughput to produce results faster.
EnSight Multimode Plate Reader
EnSight Multimode Plate Reader

Multimode Plate Readers

We offer plate readers with a wide range of modes to meet the diverse application requirements of any Cell Signaling lab. Each multimode detection plate reader features leading technologies, so no matter which instrument you choose, you can expect excellent performance for your specific assay.
  • VICTOR X Multilabel Plate Reader - a versatile, reliable system delivering multiple detection technologies on a single cost-effective platform, providing flexibility, sensitivity, and ease of use to Cell Signalling research labs of all sizes.
  • EnSpire® Multimode Plate Reader - designed to meet the demands of multiuser research environments, providing high-performance technology and flexibility for a range of applications – without compromising sensitivity.
  • EnSpire Alpha Plate Reader - proven Alpha technology at an affordable price.
  • EnSight Multimode Plate Reader - the first benchtop system to offer well imaging alongside labeled and label-free technologies, the EnSight system’s unique combination of detection modes enables you to monitor the quality of your cell samples and take an orthogonal approach to your assays.
  • EnVision®Multilabel Plate Reader - One of the fastest readers in the market, the EnVision® multilabel system includes two detectors enabling simultaneous, fast dual-wavelength reading and high-speed, sensitive laser-based TRF and Alpha technology detection. And it’s expandable, too, as your throughput and application needs change and grow.
  • ViewLux® - the top-of-the-line microplate imager that’s ideal for ultrahigh-throughput screening of compound libraries requiring the ultimate in speed, reliability, and high-quality data.
  • The MicroBeta2 System provides advanced radiometric and luminescent detection abilities for GPCRs, kinases, reporter gene assays, and traditional liquid scintillation counting. Providing coincidence counting, a unique patented configuration with two photomultiplier tubes that simultaneously detect signal, this system ensures high efficiency and extremely low background for a variety of radionuclides.
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