Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1347 Products and Services

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AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
Price Range : 339.00 USD - 9500.00 USD

Myoglobin (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Myoglobin Detection Kit is designed for detection and quantitation of human myoglobin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
List Price : 21500.00 USD
AlphaLISA immunoassay kits

Myoglobin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Myoglobin Detection Kit is designed for detection and quantitation of human myoglobin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
List Price : 2745.00 USD

IL-15 (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse Interleukin-15 (IL-15) Detection Kit is designed for detection and quantitation of mouse IL-15 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
List Price : 9500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Price Range : 461.00 USD - 26700.00 USD
AlphaLISA immunoassay kits

IL-15 (mouse) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Mouse Interleukin-15 (IL-15) Detection Kit is designed for detection and quantitation of mouse IL-15 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
List Price : 1433.00 USD
AlphaLISA immunoassay kits

Myeloperoxidase (human) AlphaLISA Detection Kit

The AlphaLISA® Human Myeloperoxidase (MPO) Detection Kit is designed for detection and quantitation of human MPO in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2745.00 USD - 21500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
List Price : 461.00 USD

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 339.00 USD - 2713.00 USD

Anti-Human IgG4 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG4. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 532.00 USD - 21900.00 USD
AlphaLisa Analyte 2

IL-15 (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-15 (IL-15) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-15 kit, but can be ordered separately.
List Price : 233.00 USD

AlphaLISA SureFire Ultra ERK 1/2 pT202/Y204, Lysat

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-ERK1/2 assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-ERK assay kit, but can also be ordered separately.
List Price : 20.00 USD
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology.
List Price : 15200.00 USD

MMP-9 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of human MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
List Price : 9500.00 USD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD

Anti-FLAG AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-FLAG antibody. This mouse monoclonal M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites.
Price Range : 554.00 USD - 22700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 461.00 USD - 26700.00 USD

α-tubulin AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for α-tubulin enables the detection and quantitation of human α-tubulin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
List Price : 18500.00 USD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
List Price : 1406.00 USD
AlphaLisa Analyte 2

ICAM-1 (human), lyophilized (AlphaLISA)

Human Intercellular Adhesion Molecule-1 (ICAM-1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human ICAM-1 kit, but can be ordered separately.
List Price : 499.00 USD

Aflatoxin AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for aflatoxin enables the quantitative determination of aflatoxin in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). 
List Price : 9200.00 USD

IL-12 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-12 enables the quantitative determination of bovine IL-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
SureFire principle

AlphaScreen SureFire SMAD2 (p-Ser465;Ser467) Assay Kit, 10,000 Points

The AlphaScreen® SureFire® p-SMAD2 (p-Ser465/467) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.
List Price : 6200.00 USD

AlphaLISA SureFire Ultra eIF4E pS209 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-eIF4 (Ser209) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-eIF4 assay kit, but can also be ordered separately.
List Price : 20.00 USD
AlphaLISA immunoassay kits

MMP-9 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of human MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
List Price : 1433.00 USD
AlphaLISA assay principle

IL-31 (Human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for IL-31 enables the quantitative determination of human IL-31 in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse IL31. Cross-reactivity with other species has not been tested.
List Price : 2520.00 USD
AlphaLISA immunoassay kits

Monocyte Chemoattractant Protein-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) Detection Kit is designed for detection and quantitation of human CCL2/MCP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1433.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

AlphaLISA Two-in-One Detection Kit: Total or Oligomerized Aβ42, 500 assay points

The AlphaLISA® Two-in-One Amyloid Beta 42 (Aβ 1-42) Detection Kit is designed for detection and quantitation of total and oligomerized human Aβ 1-42 in cell culture supernatants and cerebrospinal fluid (CSF) using a homogeneous (no-wash steps, no separation steps) assay. The kit includes standards for both oligomerized and monomeric Aβ1-42, allowing the assay to be run for accurate quantitation. The oligomerized form is provided in collaboration with Crossbeta, using their innovative oligomer stabilization technology.
List Price : 2745.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 IL-6 Detection Kit 5000 Assay Points

The AlphaPlex™ 545 (Terbium) IL-6 Detection Kit is designed for the quantitative determination of human IL6 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
List Price : 9500.00 USD

Anti-Human IgG2 AlphaLISA Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to a anti-human IgG2. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
List Price : 5300.00 USD

IgG (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgG in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 589.00 USD - 9100.00 USD
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology.
Price Range : 1407.00 USD - 15200.00 USD

Anti-Sheep IgG AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-sheep IgG antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture sheep IgG antibodies for AlphaLISA no-wash assays.
Price Range : 554.00 USD - 22700.00 USD
Alpha donor antibody coated

Anti-FLAG® Alpha Donor beads

Alpha Donor beads conjugated to anti-FLAG antibody. This bead can be used to capture FLAG-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.
Price Range : 454.00 USD - 8200.00 USD
AlphaLISA immunoassay kits

IFNγ (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IFNγ in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 608.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 608.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

Plasminogen (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Plasminogen (PLG) Detection Kit is designed for detection and quantitation of human plasminogen in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. This kit was designed to detect both plasminogen and plasmin.
List Price : 1433.00 USD
AlphaLisa Analyte 2

IFN-λ2 (human), liquid (AlphaLISA)

Human interferon lamda-2 (IFN-λ2) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human IFN-λ2 Detection Kit, but can also be ordered separately.
List Price : 226.00 USD
SureFire principle

AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
List Price : 16700.00 USD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
List Price : 15200.00 USD

MMP-9 (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of murine MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
List Price : 21500.00 USD

Anti-HRP AlphaLISA Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to anti-HRP antibody. These beads can be used to capture HRP-fusion proteins for AlphaLISA no-wash assays.
List Price : 22100.00 USD

TSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TSH enables the quantitative determination of human thyroid stimulating hormone (TSH) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLisa Analyte 2

HIV p24, lyophilized (AlphaLISA)

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) (high sensitivity) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA HIV p24 kit, but can be ordered separately.
List Price : 233.00 USD
AlphaLISA immunoassay kits

IFN-γ (porcine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
List Price : 587.00 USD

HGFR/c-MET (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human HGFR / c-MET Detection Kit is designed for detection and quantitation of HGFR / c-MET in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of HGFR. The analyte in this kit consists of the extracellular domain of human HGFR fused to the Fc region of human IgG1.
List Price : 21500.00 USD

AlphaScreen cAMP Detection Kit

AlphaScreen no-wash assay kit for quantitative detection of cAMP.
Price Range : 380.00 USD - 13000.00 USD
Alpha_nickel_Donor

Nickel Chelate Alpha Donor beads, 1 mg

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.
List Price : 454.00 USD

AlphaLISA SureFire Ultra GAPDH total Lysate

Extra control lysate for the AlphaLISA SureFire Ultra GAPDH assay. This control is already provided in the AlphaLISA SureFire Ultra GAPDH assay kit, but can also be ordered separately.
List Price : 20.00 USD

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