Alpha

OF

Eliminate the common challenges associated with assays in detection, quantitation, and binding. Our Alpha technology provides a unique beads-based platform that combines convenience, optimal performance, and superior flexibility.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
  • Distance: measure analytes and binding interactions spanning up to 200 nm or more
  • Broad range of affinities: picomolar to low millimolar, stable, or transient interactions
  • Avidity: because each bead has multiple binding sites, low affinity binding interactions can be detected using nanomolar concentrations of proteins or other binding partners

 

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1639 Products and Services

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AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

List Price: ***
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

List Price: ***
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

List Price: ***
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

List Price: ***
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra STAT1 pY701, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA-antibody-coated

Anti-rabbit IgG (Fc specific) AlphaLISA Acceptor Beads 250 µg

AlphaLISA® Acceptor beads conjugated to anti-rabbit IgG antibody. These beads can be used to capture rabbit IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 554.00 USD
alphaimmunoassay1

Renin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Renin / Prorenin Detection Kit is designed for detection and quantitation of human renin and prorenin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to equally detect both Renin and Prorenin.

List Price: 1433.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-O-linked GlcNAc Acceptor Beads, 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.

List Price: 1339.00 USD
alphaimmunoassay1

IL-2 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Interleukin-2 (IL2) Detection Kit is designed for detection and quantitation of human IL-2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA-antibody-coated

Anti-mouse IgG (Fc specific) AlphaLISA Acceptor Beads 5 mg

AlphaLISA® Acceptor beads conjugated to anti-mouse IgG antibody. These beads can be used to capture mouse IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 5500.00 USD

EGF (human), lyophilized (AlphaLISA)

Human EGF standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA EGF kit, but can be ordered separately.

List Price: 233.00 USD
alphaimmunoassay1

Residual Protein A AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA Residual Protein A Detection Kit is designed for the quantitative detection of Protein A contaminants in buffered solution using a homogeneous (no wash steps, no separation steps) assay.

List Price: 6000.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra STAT1 pY701 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

List Price: 461.00 USD

AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 8600.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-Anti-O-linked GlcNAc Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.

List Price: 24400.00 USD
alphaimmunoassay1

GM-CSF (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Detection Kit is designed for detection and quantitation of human GM-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD
AlphaLISA-antibody-coated

Anti-human IgG (Fc specific) AlphaLISA® Acceptor Beads 25 mg

AlphaLISA® Acceptor beads conjugated to anti-human IgG antibody. These beads can be used to capture human IgG antibodies. The antibody conjugated to these beads is Fc-specific, and is a mouse polyclonal antibody.

List Price: 22700.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD

AlphaLISA BRD4_BD2 / H4 K8Ac Binding Kit, 5,000 points

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain2: BRD4 (BD2) and acetylated histone H4 peptide (H4 K8Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.

List Price: 4118.00 USD
alphaimmunoassay1

IL-3 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Interleukin-3 (IL3) Detection Kit is designed for detection and quantitation of human IL-3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD
Alpha binding assay principle

EGF/EGFR AlphaLISA Binding Kit, 100 Assay Points

The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.

List Price: 420.00 USD
AL315

AlphaLISA cAMP Detection Kit, 100 Assay Points

AlphaLISA® cAMP kit for quantitative detection of cAMP. This assay can be used as a functional assay for Gs-coupled and Gi-coupled GPCR activity.

List Price: 204.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit, 10,000 points

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.

List Price: 6800.00 USD
alphaimmunoassay1

Residual Protein A AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA Residual Protein A Detection Kit is designed for the quantitative detection of Protein A contaminants in buffered solution using a homogeneous (no wash steps, no separation steps) assay.

List Price: 716.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
SureFire principle

AlphaScreen SureFire SMAD1 (p-Ser463;Ser465) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-SMAD1 (p-Ser463/465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/465) in cellular lysates using Alpha Technology.

List Price: 1542.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

List Price: 461.00 USD

AlphaLISA SureFire Ultra Total STAT3 Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 1558.00 USD
alphaimmunoassay1

GM-CSF (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Detection Kit is designed for detection and quantitation of human GM-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD
AlphaLISA-antibody-coated

Anti-HA AlphaLISA Acceptor Beads, 5 mg

AlphaLISA Acceptor beads conjugated to anti-HA (hemagglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.

List Price: 5600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

List Price: 8600.00 USD

AlphaLISA BRD4 (BD1) and H4 K5Ac Binding Assay Kit, 500 Assay Points

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain1: BRD4 (BD1) and acetylated histone H4 peptide (H4 K5Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.

List Price: 773.00 USD
Protein

IL-4 (bovine), lyophilized (AlphaLISA)

Bovine IL-4 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-4 Detection Kit, but can be ordered separately.

List Price: 226.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD

AlphaLISA Lysis Buffer, 5X (10 mL)

Pre-formulated buffer that can be used as a general lysis buffer for Alpha assays.

List Price: 130.00 USD

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