PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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AlphaLISA immunoassay kits

IL-6 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 6 (IL-6) enables the quantitative determination of porcine IL-6 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1064.00 USD - 10300.00 USD
AlphaLisa Analyte 2

Hyaluronic acid (human), lyophilized (AlphaLISA)

Human hyaluronic acid (hHA) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Hylauronic Acid Detection Kit, but can be ordered separately.
List Price : 240.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Tyr701) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Tyr701) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

AlphaScreen GST Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 180.00 USD - 13800.00 USD
AlphaLISA immunoassay kits

PD-1/PD-L1 (human) AlphaLISA Binding Kit

The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.
Price Range : 695.00 USD - 9700.00 USD
SureFire principle

AlphaScreen SureFire IKKa (p-Ser176/Ser180) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD

Anti-Human IgG4 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG4. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 565.00 USD - 23200.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-GSK-3β (Ser9) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-GSK-3β (Ser9) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

IFNβ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interferon β (IFNβ) enables the quantitative determination of porcine IFNβ in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1064.00 USD - 10300.00 USD
AlphaLisa Analyte 2

IL-8 (porcine), lyophilized (AlphaLISA)

Porcine interleukin 8 (IL-8) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IL-8 Detection Kit, but can also be ordered separately.
List Price : 706.00 USD
AlphaLISA immunoassay kits

IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 695.00 USD - 10000.00 USD
AlphaLisa Analyte 2

Galectin-9 (human), lyophilized (AlphaLISA)

Human galectin-9 (GAL-9) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human GAL-9 Detection Kit, but can also be ordered separately.
List Price : 404.00 USD
AlphaLisa Analyte 2

TNFα (cynomolgus), lyophilized (AlphaLISA)

Cynomolgus TNFα standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Cynomolgus TNFα Detection Kit, but can also be ordered separately.
List Price : 233.00 USD
alphascreen surefire total

AlphaScreen SureFire JNK 123 Total Assay Kit

The AlphaScreen® SureFire® total JNK1/2/3 assay is a sandwich immunoassay for quantitative detection of JNK1/2/3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 14300.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 695.00 USD - 12400.00 USD

AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 
Price Range : 180.00 USD - 13800.00 USD
AlphaLISA immunoassay kits

IL-4 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 4 (IL4) Detection Kit is designed for detection and quantitation of human IL4 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
List Price : 1520.00 USD
AlphaLisa Analyte 2

Biotinylated Human FCGR3A (176Val), lyophilized (AlphaLISA)

Biotinylated human FCGR3A (176Val) receptor. For use in AlphaLISA® no-wash assays.
List Price : 265.00 USD
AlphaLISA immunoassay kits

IL-12/IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.
Price Range : 696.00 USD - 19600.00 USD
AlphaLISA immunoassay kits

IL-10 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-10 enables the quantitative determination of porcine interleukin 10 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14800.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLisa Analyte 2

IL-6 (porcine), lyophilized (AlphaLISA)

Porcine interleukin 6 (IL-6) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IL-6 Detection Kit, but can also be ordered separately.
List Price : 706.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human TNFα Detection Kit

The AlphaPlex™ 645 (Samarium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 607.00 USD - 10000.00 USD
PerkinElmer

AlphaLISA SureFire Ultra SMAD1 pS463/465 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Smad1 (Ser463/465) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Smad1 assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLisa Analyte 2

Amyloid β 1-40 (human), lyophilized (AlphaLISA)

Human Amyloid β 1-40, lyophilized, 0.3 µg. Amyloid β 1-40 is included in the AlphaLISA® human Amyloid β 1-40 kits, but can also be ordered separately as a standalone item. One vial contains an amount of human Amyloid β 1-40 sufficient for performing 10 standard curves.
List Price : 247.00 USD
AlphaLISA immunoassay kits

HLA-E (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-E enables the quantitative determination of human MHC class I antigen E (HLA-E) in buffered solution, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLisa Analyte 2

IgG (mouse), lyophilized (AlphaLISA)

Mouse IgG standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA mouse IgG kits, but can also be ordered separately.
List Price : 238.00 USD
AlphaLisa Analyte 2

OX40L (human), lyophilized (AlphaLISA)

Recombinant human OX40L standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human OX40L detection kit, but can also be ordered separately.
List Price : 728.00 USD
AlphaLISA immunoassay kits

CHO-P AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.
Price Range : 759.00 USD - 6300.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF4E (Ser209) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 695.00 USD - 9700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA immunoassay kits

Myeloperoxidase (human) AlphaLISA Detection Kit

The AlphaLISA® Human Myeloperoxidase (MPO) Detection Kit is designed for detection and quantitation of human MPO in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3110.00 USD - 22800.00 USD

Anti-Mouse IgG2a (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2a. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 565.00 USD - 23200.00 USD

α-tubulin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for α-tubulin enables the detection and quantitation of human α-tubulin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 697.00 USD - 19600.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IGF Receptor β (Tyr1135/1136) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IGF Receptor β (Tyr1135/1136) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD

IL-22 (human/rat) AlphaLISA Detection kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human or rat IL-22 in serum, cell culture supernatants, and other samples.
Price Range : 695.00 USD - 10000.00 USD

AlphaScreen Histidine (Nickel Chelate) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 180.00 USD - 13800.00 USD
AlphaLISA SureFire Ultra kits

Phospho-ErbB2 (Tyr1221/1222) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD
AlphaLISA immunoassay kits

IFNα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interferon α (IFNα) enables the quantitative determination of porcine IFNα in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1064.00 USD - 10300.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p70 S6K (Thr389) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

IL-17A (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 17A (IL-17A) enables the quantitative determination of porcine IL-17A in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1064.00 USD - 10300.00 USD
PerkinElmer

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Lysate

Extra positive and negative control lysates for the AlphaLISA® SureFire® Ultra phospho-eIF2α (Ser51) assay.
List Price : 21.00 USD

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 618.00 USD - 18500.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-EGF Receptor (Tyr1068) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-EGF Receptor (Tyr1068) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL1β Detection Kit

The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 588.00 USD - 10000.00 USD

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