PerkinElmer

Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 891 Products and Services

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BRCA2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human breast cancer type 2 susceptibility protein (BRCA2) enables the quantitative determination of human BRCA2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

IL-1α (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 626.00 USD - 10000.00 USD

AlphaLISA Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

ß-NGF (human) AlphaLISA Detection Kit

The AlphaLISA® Human Beta-Nerve Growth Factor (β-NGF) Detection Kit is designed for detection and quantitation of human β-NGF in cell culture medium and serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD

BMP-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BMP-9 enables the quantitative determination of human Bone Morphogenetic Protein 9 (BMP-9) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD

Trefoil factor 3 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Trefoil Factor 3 (TFF3) Detection Kit is designed for detection and quantitation of human TFF3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD

AlphaLISA Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
Price Range : 1075.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

Testosterone AlphaLISA Detection Kit

The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 626.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 600.00 USD - 18000.00 USD
AlphaLisa Analyte 2

TFF3 (human), lyophilized (AlphaLISA)

Human Trefoil Factor 3 (TFF3) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human TFF3 kit, but can be ordered separately.
List Price : 240.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA immunoassay kits

Beef troponin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for beef troponin enables the quantitative determination of beef troponin in food samples using a homogeneous AlphaLISA assay (no wash steps). This assay kit recognizes beef, bison, lamb, and pork meat but shows low cross reacitivity with chicken meat.
Price Range : 450.00 USD - 9200.00 USD
alphascreen surefire total

AlphaScreen SureFire GAPDH Assay Kit

The AlphaScreen® SureFire® total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a cellular normalization assay.
Price Range : 1590.00 USD - 17200.00 USD

IFN-β (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon β (IFNβ) Detection Kit is designed for detection and quantitation of human IFNβ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-ß 1a subtype.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

IgG (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human IgG in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fcγ region.
Price Range : 737.00 USD - 6100.00 USD
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.
Price Range : 675.00 USD - 9800.00 USD

Anti-goat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-goat IgG antibody. These beads can be used to capture goat IgG antibodies. The antibody is Fc-specific, and is a rabbit polyclonal F(ab')2 fragment.
Price Range : 571.00 USD - 23400.00 USD

AlphaLISA anti-mouse IgG Acceptor beads

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.
Price Range : 548.00 USD - 22500.00 USD
AlphaLisa Analyte 2

Testosterone in DMSO (AlphaLISA)

Testosterone standard in 100% DMSO, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Testosterone Research kit, but can be ordered separately.
List Price : 240.00 USD
PerkinElmer

Alpha SureFire Ultra Activation Buffer B

Additional buffer for Alpha SureFire Ultra assays.
Price Range : 115.00 USD - 601.00 USD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
Price Range : 1448.00 USD - 15600.00 USD

Anti-GFP AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD
SureFire principle

AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD

AlphaLISA Anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 27 (H3K27me2-1). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1379.00 USD - 25100.00 USD

AlphaLISA Anti-O-linked GlcNAc Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.
Price Range : 1379.00 USD - 25100.00 USD

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 1075.00 USD - 3806.00 USD
AlphaLisa Analyte 2

TIM-3 (human), lyophilized (AlphaLISA)

Human TIM-3 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human TIM-3 Detection Kit, but can also be ordered separately.
List Price : 392.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 435.00 USD - 17200.00 USD

Collagen IV (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human collagen IV enables the quantitative determination of collagen IV in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

TDP-43 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TDP-43 enables the detection and quantitation of human Transactive Response DNA Binding protein 43 (TDP-43) in human serum, CSF, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
PerkinElmer
Price Range : 271.00 USD - 11100.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-Human IgG AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
AlphaLisa Analyte 2

Cry1F analyte, lyophilized (AlphaLISA)

Cry1F standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Cry1F kit, but can be ordered separately.
List Price : 321.00 USD

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD

AlphaScreen Mouse IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-mouse IgG conjugated AlphaScreen Acceptor beads.
Price Range : 175.00 USD - 13400.00 USD

CRP (rhesus) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rhesus C-reactive protein (CRP) enables the quantitative determination of rhesus CRP in buffered solution, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

AlphaLISA cAMP Detection Kit

AlphaLISA® cAMP kit for quantitative detection of cAMP. This assay can be used as a functional assay for Gs-coupled and Gi-coupled GPCR activity.
Price Range : 210.00 USD - 2859.00 USD
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1303.00 USD - 27400.00 USD
AlphaLisa Analyte 2

IGF2 (human), lyophilized (AlphaLISA)

Human Insulin-like Growth Factor 2 (IGF2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IGF2 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

IL-6 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLisa Analyte 2

TNFα (human), lyophilized (AlphaLISA)

Recombinant human TNFα, lyophilized, for use in AlphaLISA® no-wash immunoassay standard curves. This standard is already provided in ALphaLISA TNFα immunoassay kits, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

Cry1F AlphaLISA Detection Kit

The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 582.00 USD - 9000.00 USD
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLisa Analyte 2

IL-1β (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-1ß kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLisa Analyte 2

IL-17 (human), lyophilized (AlphaLISA)

Human Interleukin-17 (IL17A) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL-17 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

Aflatoxin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for aflatoxin enables the quantitative determination of aflatoxin in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 464.00 USD - 9500.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLisa Analyte 2

Lambda light chain (human), lyophilized (AlphaLISA)

Human lambda light chain standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human lambda light chain Detection Kit, but can also be ordered separately.
List Price : 287.00 USD
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD

AlphaScreen Omnibeads

Single bead instrument control that contains the chemistry of both Alpha Donor beads and AlphaScreen Acceptor beads.

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