Alpha Reagents

Biomarker/cytokine detection

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

• All-in-one-well alternative to Western Blotting
• Flexibility to use cultured cell lines, stem cells and primary cells
• Cost-effective and flexible – a single plate can accommodate any assay for any target
• Great sensitivity and dynamic range
• Easy automation
• No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal

Protein:protein interactions

Handle large biomolecular interactions with ease.

• Ease-of-use: few addition steps, fast assay development
• Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
• Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
• High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
• Conserve reagents: robust assays using nM concentrations of proteins/antibodies

Epigenetics

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex^® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex^® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

GPCR research