Alpha

OF

Eliminate the common challenges associated with assays in detection, quantitation, and binding. Our Alpha technology provides a unique beads-based platform that combines convenience, optimal performance, and superior flexibility.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
  • Distance: measure analytes and binding interactions spanning up to 200 nm or more
  • Broad range of affinities: picomolar to low millimolar, stable, or transient interactions
  • Avidity: because each bead has multiple binding sites, low affinity binding interactions can be detected using nanomolar concentrations of proteins or other binding partners

 

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1623 Products and Services

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Immunoassay buffer, 10X, 100 mL

Pre-formulated buffer optimal for most AlphaLISA analyte detection assays. This buffer is used in most AlphaLISA immunoassay kits, and can be purchased to develop your own AlphaLISA immunoassay.

List Price: 362.00 USD
alphaimmunoassay1

BDNF (human) AlphaLISA Detection Kit, 5,000 assay points

AlphaLISA no-wash immunoassay kit for detection and quantitation of Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture supernatants, and other samples.

List Price: 9500.00 USD
AL313

PEG IgM AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA anti-PEG IgM Detection Kit is designed for the quantitative determination of anti-PEG IgM antibodies in sera using a homogeneous (no wash steps, no separation steps) assay.

List Price: 16100.00 USD
AlphaLISA-antibody-coated

Anti-rabbit IgG (Fc specific) AlphaLISA Acceptor Beads 25 mg

AlphaLISA® Acceptor beads conjugated to anti-rabbit IgG antibody. These beads can be used to capture rabbit IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 22700.00 USD
AlphaLISA-antibody-coated

Anti-goat IgG (Fc specific) AlphaLISA® Acceptor Beads 250 µg

AlphaLISA® Acceptor beads conjugated to anti-goat IgG antibody. These beads can be used to capture goat IgG antibodies. The antibody is Fc-specific, and is a rabbit polyclonal F(ab')2 fragment.

List Price: 554.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads, 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1339.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 7000.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Acceptor Beads (for peptide substrate only), 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 36 (H3K36me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1339.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.

List Price: 7000.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 24400.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Acceptor Beads, 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 27 (H3K27me2-1). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1339.00 USD
AlphaLISA-antibody-coated

Anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) AlphaLISA Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 and H4 methylated on arginine residues. This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.

List Price: 1339.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.

List Price: 24400.00 USD

Neprilysin (human), lyophilized (AlphaLISA)

Neprilysin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Neprilysin kit, but can be ordered separately.

List Price: 233.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-O-linked GlcNAcAcceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.

List Price: 7000.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD
alphalisa_immunoassay_antibody_1

IgG2 (bovine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG2 in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 2019.00 USD

AlphaLISA anti-mouse IgG Acceptor beads, 5 mg

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.

List Price: 5300.00 USD
AlphaLISA-antibody-coated

Anti-human IgG (Fc specific) AlphaLISA Acceptor Beads 250 µg

AlphaLISA® Acceptor beads conjugated to anti-human IgG antibody. These beads can be used to capture human IgG antibodies. The antibody conjugated to these beads is Fc-specific, and is a mouse polyclonal antibody.

List Price: 554.00 USD
AlphaLISA assay principle

N-cadherin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human N-cadherin enables the detection and quantitation of human N-Cadherin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 1432.00 USD
alphaimmunoassay1

Leptin (mouse) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1391.00 USD
Acceptor bead

Anti-HRP AlphaLISA Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to anti-HRP antibody. These beads can be used to capture HRP-fusion proteins for AlphaLISA no-wash assays.

List Price: 538.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD
alphalisa_immunoassay_antibody_1

IgG1 (bovine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Bovine IgG1 Detection Kit is designed for detection, quantitation, and isotyping of bovine IgG1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 2019.00 USD
AlphaLISA-antibody-coated

Anti-HA AlphaLISA Acceptor Beads, 250 µg

AlphaLISA Acceptor beads conjugated to anti-HA (hemagglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.

List Price: 554.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-unmodified Histone H3 Lysine 4 (H3K4) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 unmodified against lysine 4 (H3K4). These beads can be used for no-wash AlphaLISA epigenetic eraser assays.

List Price: 7000.00 USD
AlphaLISA binding assay principle

PD-1/PD-L1 (human) AlphaLISA Binding Kit, 500 Assay Points

The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.

List Price: 1391.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads, 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 27 (H3K27ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1339.00 USD
alphascreen surefire total

AlphaScreen SureFire ERK 1/2 Total Assay Kit - 50,000 Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 16700.00 USD
AlphaLISA assay principle

N-cadherin (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human N-cadherin enables the detection and quantitation of human N-Cadherin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 432.00 USD
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit - 50,000 Points

The AlphaScreen® SureFire® total IkB assay is a sandwich immunoassay for quantitative detection of IkB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 15200.00 USD
alphaimmunoassay1

Leptin (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9200.00 USD

AlphaLISA Lysis Buffer, 5X (10 mL)

Pre-formulated buffer that can be used as a general lysis buffer for Alpha assays.

List Price: 130.00 USD

AlphaLISA anti-mouse IgG Acceptor beads, 25 mg

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.

List Price: 21900.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AL313

IgM (bovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 9700.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.

List Price: 7000.00 USD
alphaimmunoassay1

Cardiac Troponin I (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human cardiac Troponin I Detection Kit is designed for detection and quantitation of human cardiac Troponin I in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 2745.00 USD
alphaimmunoassay1

PCSK9 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) Detection Kit is designed for detection and quantitation of human PCSK9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. One antibody targets the pro-domain of PCSK9.

List Price: 1433.00 USD
AlphaLISA-antibody-coated

Anti-methyl-Histone H3 Lysine 9 (H3K9me2) AlphaLISA® Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 9 (H3K9me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 7000.00 USD
alphascreen surefire total

AlphaScreen SureFire HV Total Akt 1/2/3 Assay Kit - 96-well format

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.

List Price: 385.00 USD
Protein

N-cadherin (human), lyophilized (AlphaLISA)

Human N-cadherin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human N-cadherin Detection Kit, but can be ordered separately.

List Price: 233.00 USD
AL324

Testosterone AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 608.00 USD
alphascreen surefire total

AlphaScreen SureFire JNK 123 Total Assay Kit - 500 Points

The AlphaScreen® SureFire® total JNK1/2/3 assay is a sandwich immunoassay for quantitative detection of JNK1/2/3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 1406.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
alphaimmunoassay1

IL-17 (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® Human Interleukin-17 (IL-17, IL17A) Detection Kit is designed for detection and quantitation of human IL-17 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD
alphaimmunoassay1

Hyaluronic acid (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.

List Price: 1391.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
alphaimmunoassay1

CHO HCP (broad reactivity) AlphaLISA Detection Kit, 500 Assay Points

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.

List Price: 1391.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 461.00 USD

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