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Alpha

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Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you:

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 938 Products and Services

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AlphaLisa Analyte 2

Neprilysin (human), lyophilized (AlphaLISA)

Neprilysin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Neprilysin kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA immunoassay kits

VCAM-1 (human) AlphaLISA Detection Kit

The AlphaLISA® VCAM-1 Detection Kit is designed for detection and quantitation of Intracellular Adhesion Molecule 1 (VCAM-1) in cell culture media, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 645.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

Neprilysin (human) AlphaLISA Detection Kit

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 695.00 USD - 10100.00 USD
AlphaLisa Analyte 2

Glucagon solution (AlphaLISA)

Glucagon solution, (100 µg/mL). This glucagon standard is already provided in the AlphaLISA Glucagon immunoassay kit, but can be ordered separately as a standalone product. One vial contains an amount of Glucagon sufficient for performing 10 standard curves.
List Price : 238.00 USD

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 227.00 USD - 13800.00 USD
AlphaLISA immunoassay kits

Alpha-1 Anti-Trypsin (A1AT) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human A1AT enables the detection and quantitation of human A1AT (hA1AT) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with Human Anti-thrombin and mouse A1AT.
Price Range : 626.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of Human Programmed Cell Death Protein 1 (PD-1) in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 645.00 USD - 10100.00 USD

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1420.00 USD - 25900.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Di-Methyl-Histone H3 Lysine 4 (H3K4me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 4 (H3K4me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 695.00 USD - 12400.00 USD
AlphaLISA immunoassay kits

Corticosterone (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human corticosterone enables the quantitative determination of corticosterone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

IL-18 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-18 enables the quantitative determination of porcine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
PerkinElmer

FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 583.00 USD - 10600.00 USD
AlphaLisa Analyte 2

Leptin (human), lyophilized (AlphaLISA)

Human Leptin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human leptin kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA immunoassay kits

EGF (human) AlphaLISA Detection Kit

The AlphaLISA® EGF Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor (EGF) in cell culture media, serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 645.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

E-Cadherin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human E-cadherin enables the detection and quantitation of human E-cadherin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 446.00 USD - 9700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology.
Price Range : 409.00 USD - 16100.00 USD
AlphaLisa Analyte 2

IFNβ (porcine), lyophilized (AlphaLISA)

Porcine interferon β (IFNβ) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IFNβ Detection Kit, but can also be ordered separately.
List Price : 706.00 USD
AlphaLISA immunoassay kits

Albumin (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Albumin Detection Kit is designed for the quantitative detection of mouse albumin in serum, urine, buffered solution or cell culture medium, using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 2912.00 USD - 22800.00 USD
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 481.00 USD - 8700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

Anti-Human IgG1 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG1. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 565.00 USD - 23200.00 USD
AlphaLISA immunoassay kits

Kappa light chain (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human kappa light chain enables the quantitative determination of human kappa light chain in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD
AlphaLISA immunoassay kits

Amyloid β 1-40 (mouse/rat) AlphaLISA Detection Kit

The AlphaLISA® Mouse/Rat Amyloid beta (Aß 1-40) Detection Kit is designed for detection and quantitation of mouse or rat Aß 1-40 in serum, cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 3110.00 USD - 22800.00 USD
AlphaLisa Analyte 2

BRCA2 (human), lyophilized (AlphaLISA)

Human BRCA2 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human BRCA2 Detection Kit, but can also be ordered separately.
List Price : 233.00 USD
Alpha_Donor_bead_1
Price Range : 56.00 USD - 4776.00 USD

AlphaScreen GST Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 180.00 USD - 13800.00 USD
AlphaLisa Analyte 2

Biotinylated Human FCGR3A (176Val), lyophilized (AlphaLISA)

Biotinylated human FCGR3A (176Val) receptor. For use in AlphaLISA® no-wash assays.
List Price : 265.00 USD
alphaimmunoassay1

IL-1β (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-1β (IL1β) Detection Kit is designed for detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLisa Analyte 2

VCAM-1 (human), lyophilized (AlphaLISA)

Human VCAM-1 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA VCAM-1 kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA immunoassay kits

EPO (human) AlphaLISA Detection Kit

The AlphaLISA® Human Erythropoietin (EPO) Detection Kit is designed for detection and quantitation of human EPO in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2912.00 USD - 22800.00 USD

AlphaLISA Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.
Price Range : 1420.00 USD - 25900.00 USD
AlphaLISA immunoassay kits

SHP-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human SHP-2 enables the quantitative determination of human Src-homology domain-2 containing protein tyrosine phosphatase 2 (SHP-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 728.00 USD - 14000.00 USD
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
Price Range : 360.00 USD - 10100.00 USD
SureFire principle

AlphaScreen SureFire NFkB (p-Ser536) Assay Kit

The AlphaScreen® SureFire®p-NF-κB p65 (p-Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NF-κB p65 (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
Price Range : 492.00 USD - 17700.00 USD
AlphaLisa Analyte 2

IgG1 (mouse), lyophilized (AlphaLISA)

Lyophilized mouse IgG1 standard for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG1 isotyping kit, but can be ordered separately.
List Price : 238.00 USD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA immunoassay kits

Amyloid β 1-42 (mouse/rat) AlphaLISA Detection Kit

The AlphaLISA® Mouse/Rat Amyloid beta (Aß 1-42) Detection Kit is designed for detection and quantitation of mouse or rat Aß 1-42 in serum, cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2912.00 USD - 22800.00 USD
AlphaLisa Analyte 2

ICAM-1 (human), lyophilized (AlphaLISA)

Human Intercellular Adhesion Molecule-1 (ICAM-1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human ICAM-1 kit, but can be ordered separately.
List Price : 529.00 USD
AlphaLISA immunoassay kits

CD80 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLisa Analyte 2

NNMT (human), lyophilized (AlphaLISA)

Human Nicotinamide N-Methyltransferase (NNMT) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human NNMT Detection Kit, but can also be ordered separately.
List Price : 392.00 USD
PerkinElmer

SF UltraPlexTb CaptSure Acc Beads

CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
Price Range : 279.00 USD - 11500.00 USD

AlphaScreen General IgG (Protein A) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
Price Range : 90.00 USD - 13800.00 USD
AlphaLISA immunoassay kits

IL-1α (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 645.00 USD - 10300.00 USD

AlphaLISA Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 27 (H3K27ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1420.00 USD - 25900.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA immunoassay kits

AST (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human AST enables the quantitative determination of human aspartate transaminase (AST) in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 728.00 USD - 14000.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total p38 MAPK Assay Kit

AlphaLISA® SureFire® Ultra Total p38 MAPK assays are sandwich immunoassays for quantitative detection of p38 MAPK (phosphorylated and unphosphorylated) in cellular lysates using Alpha no-wash technology. This assay can be used for normalization of phosphorylation and other studies.
Price Range : 489.00 USD - 28400.00 USD

AlphaScreen Rabbit IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-rabbit IgG conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 180.00 USD - 13800.00 USD

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