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Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 915 Products and Services

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alphascreen surefire total

AlphaScreen SureFire Total STAT3 Assay Kit

The AlphaScreen® SureFire® total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 397.00 USD - 15700.00 USD
AlphaLISA immunoassay kits

IL-18 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
AlphaLisa Analyte 2

IL-1ß (rat), lyophilized (AlphaLISA)

Rat Interleukin 1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Rat IL-1ß kit, but can be ordered separately.
List Price : 240.00 USD
PerkinElmer

AlphaLISA SureFire Ultra SMAD1 pS463/465 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Smad1 (Ser463/465) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Smad1 assay kit, but can also be ordered separately.
List Price : 21.00 USD
alphascreen surefire total

AlphaScreen SureFire Total p38 MAPK Assay Kit

The AlphaScreen® SureFire® total p38 MAPK assay is a sandwich immunoassay for quantitative detection of p38 MAPK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 1590.00 USD - 17200.00 USD
AlphaLISA immunoassay kits

IL-8 (human) AlphaLISA Detection Kit

The AlphaLISA Human IL8 Detection Kit is designed for the quantitative determination of human IL-8 in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

Fibronectin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human fibronectin enables the quantitative determination of human fibronectin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 728.00 USD - 14000.00 USD
Protein

Ghrelin (active) (human), lyophilized (AlphaLISA)

Active human ghrelin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Active Ghrelin Detection Kit, but can also be ordered separately.
List Price : 287.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit

The AlphaScreen® SureFire® total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 1449.00 USD - 15700.00 USD
PerkinElmer

AlphaLISA SureFire IGF1 receptor pY1135/1136 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-IGFR (Tyr1135/1136) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-IGFR assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA immunoassay kits

IL-18 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-18 enables the quantitative determination of porcine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD

Anti-6xHis AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-His antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine regions at the amino- or carboxy- terminus. These beads can be used to capture His-tagged proteins and peptides for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 8900.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IL2 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 589.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

Fibronectin (human plasma) AlphaLISA Detection Kit

The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

IgG (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG (total) in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 675.00 USD - 10400.00 USD
AlphaLISA immunoassay kits

Residual Protein A AlphaLISA Detection Kit

The AlphaLISA Residual Protein A Detection Kit is designed for the quantitative detection of Protein A contaminants in buffered solution using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 737.00 USD - 6100.00 USD
PerkinElmer

AlphaLISA SureFire Insulin recepeptor pY1150/1 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Insulin Receptor β (Tyr1150/1151) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Insulin Receptor β assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA immunoassay kits

Neprilysin (human) AlphaLISA Detection Kit

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 675.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

Amyloid β 1-40 (human) AlphLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLisa Analyte 2

IgG2b (mouse), lyophilized (AlphaLISA)

Mouse IgG2b standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG2b kit, but can be ordered separately.
List Price : 231.00 USD

AlphaLISA Anti-acetyl-Histone H4 Lysine (H4Kac pan) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against acetylated lysine residues on human Histone H4 (H4Kac pan). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
PerkinElmer

Protein A AlphaPlex™ 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to Protein A, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
PerkinElmer

AlphaLISA SureFire Ultra p70 S6K pT389 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-p70 S6K (Thr389) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-p70 S6K assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA immunoassay kits

Alpha-1 Anti-Trypsin (A1AT) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human A1AT enables the detection and quantitation of human A1AT (hA1AT) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with Human Anti-thrombin and mouse A1AT.
Price Range : 608.00 USD - 9500.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL17 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 348.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLisa Analyte 2

IgA (bovine), lyophilized (AlphaLISA)

Bovine IgA standard, 10 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IgA kits, but can also be ordered separately.
List Price : 325.00 USD
AlphaLisa Analyte 2

TDP-43 (human), lyophilized (AlphaLISA)

Human TDP-43 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA TDP-43 Detection Kit, but can be ordered separately.
List Price : 300.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

Nickel Chelate AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to nickel chelate. These beads can be used to capture His-tagged proteins and peptides.
Price Range : 571.00 USD - 23400.00 USD
AlphaLISA immunoassay kits

Amyloid β 1-42 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA Buffer

AlphaLISA Dissociation Buffer

Pre-formulated buffer that can be used to dissociate Protein A from IgG in bioprocess samples. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
Price Range : 191.00 USD - 363.00 USD
AlphaLISA immunoassay kits

IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 675.00 USD - 9700.00 USD

AlphaScreen Omnibeads

Single bead instrument control that contains the chemistry of both Alpha Donor beads and AlphaScreen Acceptor beads.
AlphaLISA immunoassay kits

Amyloid ß 1-x (human) AlphaLISA Detection Kit

The AlphaLISA® Human Amyloid beta 1-x (Aß 1-x) Detection Kit is designed for detection and quantitation of human Aß 1-x in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2827.00 USD - 22100.00 USD
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit

The AlphaScreen® SureFire® total IκBαassay is a sandwich immunoassay for quantitative detection of IκBα(both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA immunoassay kits

IL-17A (serum) (mouse) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of mouse IL-17 (IL-17A homodimer) in serum, cell culture supernatants, and other samples.
Price Range : 607.00 USD - 9800.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H4 Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone H4 in cell extracts or cells in culture medium in a lysate transfer assay.
Price Range : 796.00 USD - 15600.00 USD
AlphaPlex Samarium Acceptor Beads

Protein A AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
Price Range : 570.00 USD - 23400.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

AlphaLISA Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
Price Range : 1075.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

BRCA2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human breast cancer type 2 susceptibility protein (BRCA2) enables the quantitative determination of human BRCA2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA assay principle

ANGPT2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPT2 enables the quantitative determination of human Angiopoietin 2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLisa Analyte 2

IL-8 (bovine), lyophilized (AlphaLISA)

Bovine IL-8 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-8 Detection Kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

IFNγ (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IFNγ Biotin-Free Detection Kit is designed for the quantitative detection of human IFNγ in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD

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