Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1057 Products and Services

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Anti-Human IgG1 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG1. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD
Alpha donor antibody coated

Anti-Rabbit IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-rabbit IgG antibody. This bead can be used to capture rabbit IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 467.00 USD - 8500.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 435.00 USD - 17200.00 USD

Anti-GFP AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD

Anti-methyl-Histone H3 Lysine 4 (H3K4me1-2) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

Alpha-synuclein (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Alpha-synuclein enables the quantitative determination of Alpha-synuclein in biological fluids such as serum, plasma, cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 626.00 USD - 9800.00 USD

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD

Anti-IgG1 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG1. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG1 in different sample matrices, including monkey serum.
Price Range : 553.00 USD - 22700.00 USD
AlphaLISA Binding Assay kits

EGF/EGFR AlphaLISA Binding Kit, 5,000 Assay Points

The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.
List Price : 9500.00 USD

Protein G AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.
Price Range : 571.00 USD - 23400.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 855.00 USD - 49600.00 USD
AlphaLISA immunoassay kits

Adiponectin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD

Anti-Mouse IgG2b (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2b. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-Mouse IgG AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-mouse IgG antibody. These beads are used to capture mouse IgG antibodies (targeting the Fc region).
Price Range : 570.00 USD - 23400.00 USD
AlphaLisa Analyte 2

EGF (human), lyophilized (AlphaLISA)

Human EGF standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA EGF kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLisa Analyte 2

IL-22 (human), lyophilized (AlphaLISA)

Human IL-22 standard, 1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA human/rat IL-22 detection kits, but can also be ordered separately.
List Price : 240.00 USD
AlphaPlex-545-antibody-bead2

Anti-human IgG AlphaPlex 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
AlphaLISA assay principle

FGF23 (Human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
List Price : 464.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IFN-γ Detection Kit

The AlphaPlex™ 645 (Samarium) Human Interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 447.00 USD - 9700.00 USD
AlphaLISA assay principle

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT5 Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 27600.00 USD
AlphaLISA immunoassay kits

CCL4/MIP-1ß (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 4 / Macrophage Inflammatory Protein-1beta (CCL4 / MIP-1ß) Detection Kit is designed for detection and quantitation of human CCL4/MIP-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
Alpha binding assay principle

EGF/EGFR AlphaLISA Binding Kit, 100 Assay Points

The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.
List Price : 675.00 USD

IFN-γ (porcine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
List Price : 19600.00 USD
AlphaLisa Analyte 2

IL-1ß (rat), lyophilized (AlphaLISA)

Rat Interleukin 1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Rat IL-1ß kit, but can be ordered separately.
List Price : 240.00 USD
Alpha donor antibody coated

Anti-rat IgG Alpha Donor Beads, 25 mg

Alpha Donor beads conjugated to anti-rat IgG antibody. This bead can be used to capture rat IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
List Price : 8400.00 USD
SureFire principle

AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLisa Analyte 2

Alpha-synuclein (human), lyophilized (AlphaLISA)

Human Alpha-synuclein standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Alpha-synuclein detection kit, but can also be ordered separately.
List Price : 240.00 USD
SureFire principle

AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLisa Analyte 2

IgG3 (mouse), lyophilized (AlphaLISA)

Mouse IgG3 lyophilized standard, for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG3 isotyping kits, but can be ordered separately.
List Price : 231.00 USD
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
Price Range : 349.00 USD - 9800.00 USD

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 2827.00 USD - 22100.00 USD

Anti-Mouse IgG2a (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2a. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD
AlphaLISA immunoassay kits

Il-12 (p70) (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 12 (p70) Detection Kit is designed for detection and quantitation of human IL-12 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLisa Analyte 2

CD117 (human), lyophilized (AlphaLISA)

Human CD117 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human CD117 Detection Kit, but can also be ordered separately.
List Price : 226.00 USD
AlphaPlex-545-antibody-bead2

Anti-Mouse IgG AlphaPlex™ 545 Acceptor Beads 250 µg

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody, for use in Alpha no-wash multiplexing assays.
List Price : 570.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 175.00 USD - 13400.00 USD

Anti-FITC AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to mouse monoclonal anti-FITC antibody. These beads can be used to capture FITC-tagged and fluorescein-labeled biomolecules for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD

Monocyte Chemoattractant Protein-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) Detection Kit is designed for detection and quantitation of human CCL2/MCP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

HIV p24 (high sensitivity) AlphaLISA Detection Kit

The AlphaLISA® Human Immunodeficiency Virus type-1 p24 protein (HIV p24) (high sensitivity) Detection Kit is designed for detection and quantitation of HIV p24 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

IFNγ (ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ovine interferon gamma (IFNγ) enables the quantitative determination of ovine IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

IL-1ß (rat) AlphaLISA Detection Kit

The AlphaLISA® Rat Interleukin 1ß (IL-1ß) Detection Kit is designed for detection and quantitation of rat IL-1ß in bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

CXCL4/PF4 (Human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
List Price : 2596.00 USD
AlphaLISA immunoassay kits

IL-1α (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
List Price : 626.00 USD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
Price Range : 1448.00 USD - 15600.00 USD
AlphaLISA immunoassay kits

MMP-8 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human MMP-8 enables the detection and quantitation of human MMP-8 in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP8 and pro-MMP8.
List Price : 433.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA unmodified Histone H3 Lysine 4 (H3K4) Cellular Detection Kit

This kit is designed to be used as a control for the AlphaLISA Epigenetic Cellular Detection Kits, through detection of unmodified Histone H3 Lysine 4 (H3K4) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 675.00 USD - 12000.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 Human Insulin Detection Kit, 100 Assay Points

The AlphaPlex™ 545 (Terbium) Human Insulin Detection Kit is designed for the quantitative determination of human Insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
List Price : 447.00 USD

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