Alpha

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1553 Products and Services

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SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD

AlphaLISA BRD4_BD1 / H4 K5,12Ac Binding Kit, 500 points

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain1: BRD4 (BD1) and acetylated histone H4 peptide (H4 K5,12Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.

List Price: 773.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
Alpha donor antibody coated

Anti-DNP Alpha Donor Beads

Alpha Donor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules.

List Price: 17500.00 USD

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads (25mg)

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.

List Price: 22100.00 USD

IL-8 (bovine/ovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.

List Price: 9500.00 USD

Albumin (human) AlphaLISA Detection Kit, 5,000 Assay Points

AlphaLISA no-wash immunoassay kit for detection of human serum albumin (HSA) in urine, buffered solution, or cell culture medium.

List Price: 21500.00 USD

Factor VIII (human) AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® Human Factor VIII Detection Kit is designed for detection and quantitation of Human Factor VIII in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NF?B (Ser536) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads (250 µg)

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.

List Price: 537.00 USD
AlphaLISA-antibody-coated

Anti-GST AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-GST antibody. These beads can be used to capture GST-tagged proteins and peptides in AlphaLISA no-wash assays.

List Price: 22700.00 USD
AlphaLISA immunoassay kits

IL-8 (bovine/ovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.

List Price: 433.00 USD
SureFire principle

AlphaScreen SureFire Histone H3 (p-Ser10) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-Histone H3 (Ser10) assay is a sandwich immunoassay for quantitative detection of phospho-Histone H3 (phosphorylated on Ser10) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
AlphaLISA immunoassay kits

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit, 100 Assay Points

The AlphaLISA® Human BDNF Biotin-Free Detection Kit is designed for the quantitative detection of human Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.

List Price: 432.00 USD

SureFire Ultra Activation Buffer C (50X)

Extra activation buffer for the AlphaLISA SureFire Ultra pSTAT4 assay. This buffer is already included in the AlphaLISA SureFire Ultra pSTAT4 kit, but can also be ordered separately.

List Price: 112.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IL2 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

List Price: 1433.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H4 Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone H4 in cell extracts or cells in culture medium in a lysate transfer assay.

List Price: 15100.00 USD

Albumin (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse Albumin Detection Kit is designed for the quantitative detection of mouse albumin in serum, urine, buffered solution or cell culture medium, using a homogeneous (no wash steps, no separation steps) assay.

List Price: 21500.00 USD

Amyloid ? 1-42 (high specificity) (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Amyloid beta 1-42 (Aß 1-42) (high specificity) Detection Kit is designed for detection and quantitation of human Aß 1-42 in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 21500.00 USD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
AlphaLISA immunoassay kits

IgA (mouse) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 584.00 USD

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads (5mg)

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.

List Price: 5400.00 USD
AlphaLISA immunoassay kits

IL-10 (bovine/ovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-10 enables the detection and quantitation of bovine IL-10 (bIL10) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit also detects Ovine IL-10.

List Price: 541.00 USD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
AlphaLISA immunoassay kits

Factor VIII (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® Human Factor VIII Detection Kit is designed for detection and quantitation of Human Factor VIII in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD

AlphaLISA CaptSure Acceptor Beads - 6 mL

Additional beads for Alpha SureFire Ultra assays.

List Price: 10800.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-FLAG AlphaPlex-645 Acceptor beads, 250 µg

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.

List Price: 554.00 USD
SureFire principle

AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-STAT3 (p-Ser705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Ser705) in cellular lysates using Alpha Technology.

List Price: 16700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD

IL-15 (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse Interleukin-15 (IL-15) Detection Kit is designed for detection and quantitation of mouse IL-15 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD

p24 (human HIV), lyophilized (AlphaLISA)

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) standard, lyophilized, for use in running standard curves in AlphaLISA® no-wash immunoassays. This standard is already provided in the AlphaLISA HIV p24 kit, but can be ordered separately.

List Price: 233.00 USD

VEGF-A (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

AlphaLISA® no-wash immunoassay kit for detection of mouse VEGF-A in buffer, cell culture supernatants, BALF (bronchoalveolar lavage fluid), lung homogenates, and other sample types. The analyte in this kit consists of the mouse VEGFA164.

List Price: 21500.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA di/mono-methylated-Histone H3 Lysine 27 (H3K27me2-1) Cellular Detection Kit, 5,000 Assay Points

This kit is designed for the detection of di/mono-methylated Histone H3 Lysine 27 (H3K27me2-1) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 11700.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human ICAM-1 Detection Kit, 100 Assay Points

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).

List Price: 608.00 USD
AlphaLISA-Protein-coated-bead

Protein A AlphaLISA Acceptor Beads 5 mg

AlphaLISA® Acceptor beads conjugated to Protein A. These beads can be used to capture antibodies that associate with Protein A.

List Price: 5500.00 USD
AlphaLISA immunoassay kits

Albumin (mouse) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Mouse Albumin Detection Kit is designed for the quantitative detection of mouse albumin in serum, urine, buffered solution or cell culture medium, using a homogeneous (no wash steps, no separation steps) assay.

List Price: 2745.00 USD
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads, 5mg

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.

List Price: 1356.00 USD
AlphaPlex-545-antibody-bead2

Anti-unmodified H3K4 AlphaPlex™-545 Acceptor Beads, 25 mg

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

List Price: 24300.00 USD
AlphaLISA-antibody-coated

Anti-Maltose-Binding Protein (MBP) AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-MBP (maltose-binding protein) antibody. The antibody is a rat monoclonal antibody, clone MBP 7G4. These beads can be used to capture MBP-tagged proteins for AlphaLISA no-wash assays.

List Price: 554.00 USD
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.

List Price: 1542.00 USD
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.

List Price: 2745.00 USD
AlphaLISA immunoassay kits

IL-13 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Interleukin 13 Detection Kit is designed for detection and quantitation of human IL-13 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD
AlphaLISA immunoassay kits

IgA (mouse) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 1378.00 USD
AlphaLISA-antibody-coated

Anti-Histone H3 (C-term) AlphaLISA Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L. Use in conjunction with biotinylated anti-H3K79me2 (AL148).

List Price: 24400.00 USD
Protein

IL-8 (bovine), lyophilized (AlphaLISA)

Bovine IL-8 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-8 Detection Kit, but can be ordered separately.

List Price: 233.00 USD
AlphaLISA immunoassay kits

TNF? (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNF? in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 1942.00 USD

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