PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-12 of 1037 Products & Services

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  • Trefoil factor 3 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Trefoil Factor 3 (TFF3) Detection Kit is designed for detection and quantitation of human TFF3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • Assay principle for the AlphaLISA PCSK9/LDLR binding kit

    PCSK9/LDLR (human) AlphaLISA Binding Kit

    This kit is designed to assess binding and inhibition of human PCSK9 and LDLR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics using competition assays.
    Price Range : 2502.00 USD - 17900.00 USD
  • AlphaLisa Analyte 2

    PCSK9 (human), lyophilized (AlphaLISA)

    Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human PCSK9 kit, but can be ordered separately.
    List Price : 257.00 USD
  • Il-12 (p70) (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin 12 (p70) Detection Kit is designed for detection and quantitation of human IL-12 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • AlphaLisa Analyte 2

    IFN-β (human), lyophilized (AlphaLISA)

    Human Interferon β (IFNβ) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The standard corresponds to the IFN-ß 1a subtype . This standard is already provided in the AlphaLISA human IFNβ kit, but can be ordered separately.
    List Price : 257.00 USD
  • Amyloid β 1-42 (high specificity) (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Amyloid beta 1-42 (Aß 1-42) (high specificity) Detection Kit is designed for detection and quantitation of human Aß 1-42 in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3036.00 USD - 23700.00 USD
  • AlphaLisa Analyte 2

    MMP13 (human), lyophilized (AlphaLISA)

    Human MMP13 (matrix metalloproteinase-13) standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA MMP-13 detection kits, but can also be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

    The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
    Price Range : 504.00 USD - 29200.00 USD
  • AlphaLISA immunoassay kits

    MMP-9 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of human MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
    Price Range : 1585.00 USD - 10500.00 USD
  • AlphaLISA Biotin-anti-Histone H4 Antibody

    AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
    Price Range : 1154.00 USD - 10400.00 USD
  • AlphaLISA immunoassay kits

    CD70 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD70 enables the quantitative determination of human CD70 in buffer, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 11100.00 USD
  • AlphaLisa Analyte 2

    DNA (liquid), AlphaLISA

    DNA standard for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Host Cell Residual DNA kit, but can be ordered separately.
    List Price : 257.00 USD
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