PerkinElmer

Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 987 Products and Services

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AlphaLISA immunoassay kits

GDNF (human) AlphaLISA Detection kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human GDNF (glial cell-derived neurotrophic factor) in serum, plasma, cell culture supernatants, and other samples.
Price Range : 626.00 USD - 9700.00 USD

HIV p24 (high sensitivity) AlphaLISA Detection Kit

The AlphaLISA® Human Immunodeficiency Virus type-1 p24 protein (HIV p24) (high sensitivity) Detection Kit is designed for detection and quantitation of HIV p24 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLisa Analyte 2

GDNF (human), lyophilized (AlphaLISA)

Human GDNF (glial cell-derived neurotrophic factor) standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA GDNF detection kits, but can also be ordered separately.
List Price : 240.00 USD

IL-2 (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD

Insulin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 675.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

E-Cadherin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human E-cadherin enables the detection and quantitation of human E-cadherin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
List Price : 1433.00 USD
AlphaLisa Analyte 2

PAI-1 (human), lyophilized (AlphaLISA)

Human Plasminogen Activator Inhibitor-1 (PAI-1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The analyte consists of the active glycosylated human PAI-1. This standard is already provided in the AlphaLISA human PAI-1 kit, but can be ordered separately.
List Price : 514.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IP10 Detection Kit

The AlphaPlex Terbium (Tb) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 668.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

HIV p24 AlphaLISA Biotin-Free Detection Kit

The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD
AlphaLisa Analyte 2

EPO (human), lyophilized (AlphaLISA)

Human Erythropoietin (EPO) standard, lyophilized, for use in running standard curves in AlphaLISA® no-wash immunoassays. This standard is already provided in the AlphaLISA human EPO kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA Bromodomain Binding Assay kits

AlphaLISA BRD4_BD2 / H4 K5,8,12Ac Binding Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain2: BRD4 (BD2) and acetylated histone H4 peptide (H4 K5,8,12Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 796.00 USD - 4242.00 USD
AlphaLisa Analyte 2

TAU (human), lyophilized (AlphaLISA)

Human Microtubule-associated Protein Tau (TAU) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human TAU kit, but can be ordered separately.
List Price : 514.00 USD
AlphaLISA immunoassay kits

GM-CSF (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Detection Kit is designed for detection and quantitation of mouse GM-CSF in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2827.00 USD - 22100.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 Human Insulin Detection Kit

The AlphaPlex™ 545 (Terbium) Human Insulin Detection Kit is designed for the quantitative determination of human Insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 447.00 USD - 9700.00 USD

TGF-β1 (human) AlphaLISA Biotin-Free Detection Kit, 5,000 Assay Points

The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
List Price : 9500.00 USD
AlphaLISA immunoassay kits

E-Cadherin (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human E-cadherin enables the detection and quantitation of human E-cadherin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
List Price : 433.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA Bromodomain Binding Assay kits

AlphaLISA BRD4 (BD2) and H4 K5Ac Binding Assay Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain2: BRD4 (BD2) and acetylated histone H4 peptide (H4 K5Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 796.00 USD - 4242.00 USD
AlphaLISA immunoassay kits

Nidogen-1 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human Nidogen-1 enables the detection and quantitation of Human Nidogen-1 (NID-1; also known as Entactin) in human serum, plasma, and, cell lysyate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
List Price : 433.00 USD
Alpha donor antibody coated

Anti-DIG Alpha Donor beads

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.
Price Range : 467.00 USD - 8500.00 USD
AlphaLISA immunoassay kits

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
Protein

MANF (human) lyophilized (AlphaLISA)

Human MANF standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human MANF Detection Kit, but can be ordered separately.
List Price : 295.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human Insulin Detection Kit

The AlphaPlex™ 645 (Samarium) Human Insulin Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 447.00 USD - 9700.00 USD
AlphaLisa Analyte 2

IL-7 (human), lyophilized (AlphaLISA)

Human Interleukin 7 (IL7) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL7 kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLisa Analyte 2

PTEN (human), lyophilized (AlphaLISA)

Human PTEN standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human PTEN Detection Kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLisa Analyte 2

D-dimer (human), lyophilized (AlphaLISA)

Human D-dimer standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human D-dimer kit, but can be ordered separately.
List Price : 514.00 USD
PerkinElmer

AlphaLISA BRD4_BD2 / H4 K8Ac Binding Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain2: BRD4 (BD2) and acetylated histone H4 peptide (H4 K8Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 796.00 USD - 4242.00 USD
AlphaLisa Analyte 2

VEGF (human), lyophilized (AlphaLISA)

Human VEGF, lyophilized, 0.3 µg. The VEGF analyte corresponds to VEGF165 (isoform d). VEGF is included in the AlphaLISA® human VEGF kits, but can also be ordered separately as a standalone item. One vial contains an amount of human VEGF sufficient for performing 10 standard curves.
List Price : 240.00 USD
PerkinElmer

AlphaLISA BRD4_BD1 / H4 K5,8,12Ac Binding Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain1: BRD4 (BD1) and acetylated histone H4 peptide (H4 K5,8,12Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 796.00 USD - 4242.00 USD
AlphaLISA immunoassay kits

IgM (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgM antibodies in different sample matrices, including cell culture media. The IgM subtype is the first antibody found during an immune response and is responsible for agglutination and cytolytic reactions.
Price Range : 675.00 USD - 9400.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL1β Detection Kit

The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 571.00 USD - 9700.00 USD

G-CSF (human) AlphaLISA Detection Kit

The AlphaLISA® Human Granulocyte Colony-Stimulating Factor (G-CSF) Detection Kit is designed for detection and quantitation of human G-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

Price Range : 1476.00 USD - 9800.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IFN-γ Detection Kit

The AlphaPlex™ 545 (Terbium) human interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 447.00 USD - 9700.00 USD
AlphaLisa Analyte 2

Troponin I (human), lyophilized (AlphaLISA)

Human Troponin I, lyophilized, for use as a standard for running standard curves in AlphaLISA no-wash immunoassays. This standard is already included in AlphaLISA Troponin kits, but can be ordered separately.
List Price : 514.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 435.00 USD - 17200.00 USD
AlphaLISA immunoassay kits

IL-18 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-18 enables the quantitative determination of bovine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human/Rat IL22 Detection kit

The AlphaPlex 545 (Terbium) Human / Rat IL22 Detection Kit is designed for the quantitative determination of human or rat IL22 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 626.00 USD - 9700.00 USD
AlphaLisa Analyte 2

p53 (human), lyophilized (AlphaLISA)

p53 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human p53 Detection Kit, but can be ordered separately.
List Price : 300.00 USD
Alpha donor antibody coated

Anti-DNP Alpha Donor Beads

Alpha Donor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules.
Price Range : 1545.00 USD - 18000.00 USD
AlphaLISA immunoassay kits

BMP-7 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human BMP-7 enables the detection and quantitation of human BMP-7 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
List Price : 1433.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 1449.00 USD - 15700.00 USD
AlphaLISA Bromodomain Binding Assay kits

AlphaLISA BRD4_BD1 / H4 K5,12Ac Binding Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain1: BRD4 (BD1) and acetylated histone H4 peptide (H4 K5,12Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 796.00 USD - 4242.00 USD

Anti-6xHis AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-His antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine regions at the amino- or carboxy- terminus. These beads can be used to capture His-tagged proteins and peptides for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD

Biotin-anti-H3K79me2 antibody

Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLisa Analyte 2

PSA (human), lyophilized (AlphaLISA)

Human Prostate-Specific Antigen (PSA) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human PSA kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

TNFα (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 626.00 USD - 10000.00 USD
AlphaLisa Analyte 2

IL-13 (bovine), lyophilized (AlphaLISA)

Bovine IL-13 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Bovine IL-13 Detection Kit, but can also be ordered separately.
List Price : 226.00 USD
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD

CD117 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD117 enables the quantitative determination of human CD117 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 175.00 USD - 13400.00 USD

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