Alpha

OF

Eliminate the common challenges associated with assays in detection, quantitation, and binding. Our Alpha technology provides a unique beads-based platform that combines convenience, optimal performance, and superior flexibility.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
  • Distance: measure analytes and binding interactions spanning up to 200 nm or more
  • Broad range of affinities: picomolar to low millimolar, stable, or transient interactions
  • Avidity: because each bead has multiple binding sites, low affinity binding interactions can be detected using nanomolar concentrations of proteins or other binding partners

 

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1799 Products and Services

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AlphaLISA assay principle

IL-17A (bovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).

List Price: 18500.00 USD

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit for 500 Assay Points

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology

List Price: 1407.00 USD
AlphaLISA assay principle

IL-17A (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).

List Price: 656.00 USD
Epi_Cell_based_detection

AlphaLISA Acetylated Histone H3 Lysine 9 (H3K9ac) Cellular Detection Kit, 500 Assay Points

This kit is designed for the detection of acetylated Histone H3 Lysine 9 (H3K9ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 2035.00 USD

AlphaLISA anti-p53K382ac Acc beads, LQC

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

Product not available online
alphalisa_immunoassay_antibody_1

IgG (bovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG (total) in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 10100.00 USD

AlphaLISA dimethylated-Histone H3 Lysine 79 (H3K79me2) Cellular Detection Kit, bulk orders

This kit is designed for the detection of di-methylated Histone H3 Lysine 79 (H3K79me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Product not available online
AlphaLISA-antibody-coated

Anti-rabbit IgG (Fc specific) AlphaLISA Acceptor Beads 5 mg

AlphaLISA® Acceptor beads conjugated to anti-rabbit IgG antibody. These beads can be used to capture rabbit IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 5500.00 USD

Progesterone AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD

IL-1α (bovine), lyophilized (AlphaLISA)

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 316.00 USD

AlphaScreen SureFire cJun (p-Ser73) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-cJun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser73) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
Epi_Cell_based_detection

AlphaLISA dimethylated-Histone H3 Lysine 79 (H3K79me2) Cellular Detection Kit, 5,000 Assay Points

This kit is designed for the detection of di-methylated Histone H3 Lysine 79 (H3K79me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 11700.00 USD

p62 (human) AlphaLISA Detection Kit, bulk orders

AlphaLISA® no-wash immunoassay kit for detection of human p62 (SQSTM1, Sequestosome 1) in buffered solution or cell lysates. The analyte in this kit consists of recombinant p62 fused to glutathione S-transferase (GST).

Product not available online
Epi_Cell_based_detection

AlphaLISA di/mono-methylated-Histone H3 Lysine 27 (H3K27me2-1) Cellular Detection Kit, 500 Assay Points

This kit is designed for the detection of di/mono-methylated Histone H3 Lysine 27 (H3K27me2-1) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 2035.00 USD
AL313

PEG IgM AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA anti-PEG IgM Detection Kit is designed for the quantitative determination of anti-PEG IgM antibodies in sera using a homogeneous (no wash steps, no separation steps) assay.

List Price: 716.00 USD
AlphaLISA-antibody-coated

Anti-rabbit IgG (Fc specific) AlphaLISA Acceptor Beads 25 mg

AlphaLISA® Acceptor beads conjugated to anti-rabbit IgG antibody. These beads can be used to capture rabbit IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 22700.00 USD

Anti-bovine IgM AlphaLISA Acceptor Beads, 25 mg

AlphaLISA Acceptor beads conjugated to anti-bovine IgM antibody. These beads can be used to capture bovine IgM antibodies.

List Price: 25400.00 USD

Progesterone AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD
AlphaPlex-545-antibody-bead2

Anti-Mouse IgG AlphaPlex™ 545 Acceptor Beads 5 mg

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody, for use in Alpha no-wash multiplexing assays.

List Price: 5500.00 USD

AlphaScreen SureFire cJun (p-Ser63) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-cJun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser63) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
alphalisa_immunoassay_antibody_1

IgG1 (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® Bovine IgG1 Detection Kit is designed for detection, quantitation, and isotyping of bovine IgG1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 632.00 USD

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD

AlphaScreen SureFire Phospho-p38 MAPK Kit for 500 Assay Points

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 1543.00 USD
Epi_Cell_based_detection

AlphaLISA dimethylated-Histone H3 Lysine 79 (H3K79me2) Cellular Detection Kit, 100 Assay Points

This kit is designed for the detection of di-methylated Histone H3 Lysine 79 (H3K79me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 584.00 USD

AlphaScreen General IgG (Protein A) Detection Kit, 500 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

List Price: 170.00 USD

IL-17A (mouse), lyophilized (AlphaLISA)

Mouse IL-17A standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA mouse IL-17A kits, but can also be ordered separately.

List Price: 233.00 USD

Amyloid β 1-42 (human), lyophilized (AlphaLISA)

Human Amyloid beta 1-42 (Aß 1-42) (high specificity) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human Aß 1-42 kit, but can be ordered separately.

List Price: 499.00 USD
AL313

PEG IgM AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA anti-PEG IgM Detection Kit is designed for the quantitative determination of anti-PEG IgM antibodies in sera using a homogeneous (no wash steps, no separation steps) assay.

List Price: 2678.00 USD
AlphaLISA-antibody-coated

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads 250 µg

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 554.00 USD

Anti-bovine IgM AlphaLISA Acceptor Beads, 5 mg

AlphaLISA Acceptor beads conjugated to anti-bovine IgM antibody. These beads can be used to capture bovine IgM antibodies.

List Price: 5700.00 USD

IL-2 (human) AlphaLISA Biotin-Free Detection Kit, 100 Assay Points

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

List Price: 608.00 USD
AlphaPlex-645-Samarium-Antibody

Anti-Human IgG AlphaPlex 645 (Sm) Acceptor beads, 250 µg

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.

List Price: 554.00 USD

Prolactin (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Prolactin Detection Kit is designed for detection and quantitation of human Prolactin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD

AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit, 50,000 points

The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

List Price: 16700.00 USD

Amyloid ß 1-x (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Amyloid beta 1-x (Aß 1-x) Detection Kit is designed for detection and quantitation of human Aß 1-x in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 21500.00 USD

IL-7 (human), lyophilized (AlphaLISA)

Human Interleukin 7 (IL7) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL7 kit, but can be ordered separately.

List Price: 233.00 USD

IFNγ (bovine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IFNγ in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 1942.00 USD
AL313

IgM (bovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

List Price: 9700.00 USD

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD

Amyloid β 1-42 (mouse/rat) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Mouse/Rat Amyloid beta (Aß 1-42) Detection Kit is designed for detection and quantitation of mouse or rat Aß 1-42 in serum, cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 2745.00 USD
Epi_Cell_based_detection

AlphaLISA Tri-Methyl-Histone H3 Lysine 27 (H3K27me3) Cellular Detection Kit, 100 Assay Points

This kit is designed for the detection of tri-methylated Histone H3 Lysine 27 (H3K27me3) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 584.00 USD
alphascreen_protein_coated_acceptor_bead

AlphaScreen Protein A Acceptor beads, 5 mg

AlphaScreen Acceptor beads conjugated to Protein A.

List Price: 1627.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.

List Price: 8600.00 USD

p62 (human) AlphaLISA Detection Kit, 500 Assay Points

AlphaLISA® no-wash immunoassay kit for detection of human p62 (SQSTM1, Sequestosome 1) in buffered solution or cell lysates. The analyte in this kit consists of recombinant p62 fused to glutathione S-transferase (GST).

List Price: 2665.00 USD
Epi_Cell_based_detection

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit, 5,000 Assay Points

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 11700.00 USD
AlphaLISA-Protein-coated-bead

Protein G AlphaLISA Acceptor Beads 25 mg

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.

List Price: 22700.00 USD
Alpha_Protein_Donor_bead

Protein A Alpha Donor beads, 25 mg

Alpha Donor beads conjugated to Protein A. This bead can be used to capture antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.

List Price: 8200.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads, 250 ug

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1339.00 USD

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology.

List Price: 6200.00 USD

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