PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-12 of 1040 Products & Services

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  • AlphaLisa Analyte 2

    CXCL9/MIG (human), lyophilized (AlphaLISA)

    Human C-X-C Motif Chemokine 9 / Monokine Induced by Gamma Interferon (CXCL9 / MIG) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CXCL9/MIG kit, but can be ordered separately.
    List Price : 552.00 USD
  • AlphaLisa Analyte 2

    TGF-β1 (human), lyophilized (AlphaLISA)

    Human TGF-β1 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human TGF-β1 kit, but can be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA immunoassay kits

    Testosterone AlphaLISA Detection Kit

    The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 672.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    TGF-β1 (human) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
    Price Range : 465.00 USD - 10200.00 USD
  • AlphaLISA immunoassay kits

    Progesterone AlphaLISA Detection Kit

    The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 724.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    ICAM-1 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Intercellular Adhesion Molecule-1 (ICAM-1) Detection Kit is designed for detection and quantitation of human ICAM-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of ICAM-1. The analyte in this kit consists of the extracellular domain of human ICAM-1.
    Price Range : 3036.00 USD - 23700.00 USD
  • Alpha-Unconjugated-Donor-bead

    AlphaScreen Unconjugated Donor Beads

    Alpha Unconjugated Donor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own Alpha Donor beads for use in Alpha no-wash assays.
    Price Range : 375.00 USD - 10500.00 USD
  • AlphaLisa Analyte 2

    Neprilysin (human), lyophilized (AlphaLISA)

    Neprilysin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Neprilysin kit, but can be ordered separately.
    List Price : 257.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-MEK 1 Kit

    The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • AlphaLisa Analyte 2

    Frataxin (human), lyophilized (AlphaLISA)

    Human frataxin, lyophilized, 0.3 µg. Human frataxin standard is already included in the AlphaLISA human frataxin kits, but can also be ordered separately as a standalone item. One vial contains an amount of human frataxin sufficient for performing 10 standard curves.
    List Price : 258.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

    The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
    Price Range : 504.00 USD - 29200.00 USD
  • AlphaLISA immunoassay kits

    PEG IgM AlphaLISA Detection Kit

    The AlphaLISA anti-PEG IgM Detection Kit is designed for the quantitative determination of anti-PEG IgM antibodies in sera using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 792.00 USD - 17800.00 USD
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