Alpha

OF

Eliminate the common challenges associated with assays in detection, quantitation, and binding. Our Alpha technology provides a unique beads-based platform that combines convenience, optimal performance, and superior flexibility.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
  • Distance: measure analytes and binding interactions spanning up to 200 nm or more
  • Broad range of affinities: picomolar to low millimolar, stable, or transient interactions
  • Avidity: because each bead has multiple binding sites, low affinity binding interactions can be detected using nanomolar concentrations of proteins or other binding partners

 

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1639 Products and Services

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AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

List Price: ***
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

List Price: ***
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.

List Price: 1558.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra STAT1 pY701, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD

AlphaLISA anti-mouse IgG Acceptor beads, 250 µg

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.

List Price: 532.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.

List Price: 461.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD

AlphaLISA anti-mouse IgG Acceptor beads, 25 mg

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.

List Price: 21900.00 USD

VEGF-C (human), lyophilized (AlphaLISA)

Recombinant human VEGF-C, lyophilized, for use in AlphaLISA no-wash immunoassay standard curves. This standard is already provided in ALphaLISA VEGF-C immunoassay kits, but can be ordered separately.

List Price: 233.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD
AlphaLISA-antibody-coated

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1393.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-MEK1 (Ser218/222) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.

List Price: 1558.00 USD
AlphaLISA-antibody-coated

Anti-mouse IgG (Fc specific) AlphaLISA Acceptor Beads 250 µg

AlphaLISA® Acceptor beads conjugated to anti-mouse IgG antibody. These beads can be used to capture mouse IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.

List Price: 554.00 USD
alphaimmunoassay1

IFN-α (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.

List Price: 1433.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 1558.00 USD
AlphaLISA-antibody-coated

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 25400.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra STAT1 pY701, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

List Price: 26800.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 36 (H3K36me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 7000.00 USD
Epi_Cell_based_detection

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit, 5,000 Assay Points

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 11700.00 USD
AlphaLISA-antibody-coated

Anti-HA AlphaLISA Acceptor Beads, 25 mg

AlphaLISA Acceptor beads conjugated to anti-HA (hemagglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.

List Price: 22800.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

List Price: 8600.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

List Price: 26800.00 USD
AlphaLISA-antibody-coated

Anti-methyl-Histone H3 Lysine 4 (H3K4me1-2) AlphaLISA Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

List Price: 1339.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra STAT1 pY701 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) HV Assay Kit - 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-unmodified Histone H3 Lysine 9 /Lysine 27 (H3K9/K27) Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.

List Price: 24400.00 USD

AlphaLISA anti-mouse IgG Acceptor beads, 5 mg

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.

List Price: 5300.00 USD
Epi_Cell_based_detection

AlphaLISA Di-Methyl-Histone H3 Lysine 4 (H3K4me2) Cellular Detection Kit, 100 Assay Points

This kit is designed for the detection of di-methylated Histone H3 Lysine 4 (H3K4me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

List Price: 584.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.

List Price: 461.00 USD

VEGFD/FIGF (human), lyophilized (AlphaLISA)

Recombinant VEGF-D analyte for running standard curves in AlphaLISA no-wash immunoassays. This standard is provided in AlphaLISA VEGFD no-wash immunoassay kits, but can also be ordered separately. One vial contains enough reagent to run 5 standard curves using the AlphaLISA VEGF-D protocol.

List Price: 233.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

List Price: 26800.00 USD

AlphaScreen cAMP Detection Kit, 1,000 assay points

AlphaScreen no-wash assay kit for quantitative detection of cAMP.

List Price: 380.00 USD

p24 (human HIV), lyophilized (AlphaLISA)

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) standard, lyophilized, for use in running standard curves in AlphaLISA® no-wash immunoassays. This standard is already provided in the AlphaLISA HIV p24 kit, but can be ordered separately.

List Price: 233.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Thr1343) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
AlphaLISA-antibody-coated

Anti-Histone H3 (C-term) AlphaLISA Acceptor Beads, 250 µg

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L. Use in conjunction with biotinylated anti-H3K79me2 (AL148).

List Price: 1339.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra STAT1 pS727, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA-antibody-coated

Anti-FLAG AlphaLISA Acceptor Beads 5 mg

AlphaLISA® Acceptor beads conjugated to anti-FLAG antibody. This mouse monoclonal M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites.

List Price: 5500.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
AlphaLISA-antibody-coated

AlphaLISA Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.

List Price: 7000.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads (5mg)

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.

List Price: 5400.00 USD

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