Alpha

OF

Eliminate the common challenges associated with assays in detection, quantitation, and binding. Our Alpha technology provides a unique beads-based platform that combines convenience, optimal performance, and superior flexibility.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
  • Distance: measure analytes and binding interactions spanning up to 200 nm or more
  • Broad range of affinities: picomolar to low millimolar, stable, or transient interactions
  • Avidity: because each bead has multiple binding sites, low affinity binding interactions can be detected using nanomolar concentrations of proteins or other binding partners

 

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 1608 Products and Services

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SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-cJun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser63) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit, 50,000 points

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.

List Price: 16700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit for 10,000 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit for 500 Assay Points

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
alphaimmunoassay1

Factor VIII (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® Human Factor VIII Detection Kit is designed for detection and quantitation of Human Factor VIII in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.

List Price: 1433.00 USD
alphaimmunoassay1

Neprilysin (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 461.00 USD
alphaimmunoassay1

Insulin (human) AlphaLISA Detection Kit, 5000 assay points

The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9200.00 USD

AlphaLISA NaCl Buffer, 5X (100 mL)

Pre-formulated buffer that can be used to dissociate Protein A from IgG in bioprocess samples. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.

List Price: 352.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Ser473) research kit, 50,000 points

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 16700.00 USD
alphaimmunoassay1

CHO HCP (broad reactivity) AlphaLISA Detection Kit, 500 Assay Points

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.

List Price: 1391.00 USD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p38 MAPK (pT180/Y182) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
alphalisa_immunoassay_antibody_1

IgG2b (mouse) isotyping AlphaLISA Detection Kit, 500 Assay Points

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG2b antibodies in cell culture media and non-mouse serum.

List Price: 1378.00 USD
alphaimmunoassay1

Insulin (human) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.

List Price: 420.00 USD
alphaimmunoassay1

PCSK9 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) Detection Kit is designed for detection and quantitation of human PCSK9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. One antibody targets the pro-domain of PCSK9.

List Price: 9500.00 USD
alphaimmunoassay1

Neprilysin (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 608.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit, 10,000 points

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.

List Price: 6800.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD

AlphaLISA cGMP Detection Kit, 500 Assay Points

AlphaLISA® cGMP kit for quantitative detection of cGMP.

List Price: 1433.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit - 10,000 Assay Points

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

List Price: 8600.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.

List Price: 6200.00 USD
alphaimmunoassay1

Hyaluronic acid (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.

List Price: 9200.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
alphalisa_immunoassay_antibody_1

IgG4 (human) Pharmacokinetic AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human IgG4 Pharmacokinetic Detection Kit is designed for the quantitative determination of human IgG4 in monkey serum and other samples, using a homogeneous (no wash steps, no separation steps) assay.

List Price: 9200.00 USD
alphaimmunoassay1

Fibronectin (human plasma) AlphaLISA Detection Kit, 5000 assay points

The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

List Price: 9200.00 USD
alphascreen surefire total

AlphaScreen SureFire JNK 123 Total Assay Kit - 500 Points

The AlphaScreen® SureFire® total JNK1/2/3 assay is a sandwich immunoassay for quantitative detection of JNK1/2/3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 1406.00 USD
alphascreen surefire total

AlphaScreen SureFire p53 Total Assay Kit - 50,000 Points

The AlphaScreen® SureFire® total p53 assay is a sandwich immunoassay for quantitative detection of p53 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 15200.00 USD
SureFire principle

AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD

AlphaLISA NaCl Buffer, 5X (10 mL)

Pre-formulated buffer that can be used to dissociate Protein A from IgG in bioprocess samples. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.

List Price: 185.00 USD
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA SurefireUltra Principal

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

List Price: 26700.00 USD
alphaimmunoassay1

CHO-P AlphaLISA Detection Kit, 5,000 assay points

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.

List Price: 6000.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology.

List Price: 1407.00 USD
AlphaLISA_SureFire_Ultra_total

AlphaLISA SureFire Ultra Total p38 MAPK Assay Kit - 50,000 Assay Points

AlphaLISA® SureFire® Ultra Total p38 MAPK assays are sandwich immunoassays for quantitative detection of p38 MAPK (phosphorylated and unphosphorylated) in cellular lysates using Alpha no-wash technology. This assay can be used for normalization of phosphorylation and other studies.

List Price: 26700.00 USD
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit - 500 Points

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology.

List Price: 1407.00 USD

IL-7 (human), lyophilized (AlphaLISA)

Human Interleukin 7 (IL7) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL7 kit, but can be ordered separately.

List Price: 233.00 USD
AlphaLISA-antibody-coated

Anti-Human IgG4 AlphaLISA Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to a anti-human IgG4. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.

List Price: 21900.00 USD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit - 50,000 Points

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.

List Price: 15200.00 USD
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads, 1 mg

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.

List Price: 454.00 USD
alphaimmunoassay1

Growth Hormone (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Growth Hormone Detection Kit is designed for detection and quantitation of human growth hormone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

List Price: 9500.00 USD
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit - 50,000 Points

The AlphaScreen® SureFire® total IkB assay is a sandwich immunoassay for quantitative detection of IkB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

List Price: 15200.00 USD

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