PerkinElmer

Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 988 Products and Services

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AlphaLisa Analyte 2

BMP-7 (human), lyophilized (AlphaLISA)

Human BMP-7 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human BMP-7 Detection Kit, but can be ordered separately.
List Price : 233.00 USD
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.
Price Range : 675.00 USD - 9800.00 USD

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 675.00 USD - 9800.00 USD
AlphaLISA assay principle

IL-31 (Human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for IL-31 enables the quantitative determination of human IL-31 in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse IL31. Cross-reactivity with other species has not been tested.
List Price : 2596.00 USD
AlphaLISA immunoassay kits

COL1A1 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human COL1A1 enables the detection and quantitation of human COL1A1 in serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
List Price : 433.00 USD
Protein

IL-31 (Human) lyophilized (AlphaLISA)

Human IL-31 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-31 Detection Kit, but can be ordered separately.
List Price : 295.00 USD
AlphaLisa Analyte 2

C-peptide (human), lyophilized (AlphaLISA)

Recombinant human C-peptide, lyophilized, for use in AlphaLISA® no-wash immunoassay standard curves. This standard is already provided in ALphaLISA C-peptide immunoassay kits, but can be ordered separately.
List Price : 233.00 USD
AlphaLisa Analyte 2

Human IgG, lyophilized (AlphaLISA)

Human IgG, lyophilized, 3 µg. Human IgG is included in the AlphaLISA® human IgG kits, but can also be ordered separately as a standalone item. One vial contains an amount of human IgG sufficient for performing 10 standard curves.
List Price : 240.00 USD

IgM (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgM antibodies in different sample matrices, including cell culture media. The IgM subtype is the first antibody found during an immune response and is responsible for agglutination and cytolytic reactions.
Price Range : 675.00 USD - 9400.00 USD
AlphaLISA immunoassay kits

IL-2 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-2 (IL-2) Detection Kit is designed for detection and quantitation of mouse IL-2 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaPlex-545-antibody-bead2

Anti-rabbit IgG AlphaPlex-545 (Tb) Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-rabbit IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD

IL-31 (Human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for IL-31 enables the quantitative determination of human IL-31 in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse IL31. Cross-reactivity with other species has not been tested.
List Price : 18000.00 USD
AlphaLISA immunoassay kits

BMP-7 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human BMP-7 enables the detection and quantitation of human BMP-7 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
List Price : 1433.00 USD

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLISA immunoassay kits

ApoB (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ApoB enables the quantitative determination of human apolipoprotein B in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD
AlphaLISA immunoassay kits

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
Price Range : 675.00 USD - 9400.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human/Rat IL22 Detection kit

The AlphaPlex 545 (Terbium) Human / Rat IL22 Detection Kit is designed for the quantitative determination of human or rat IL22 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 626.00 USD - 9700.00 USD

IL-1RA (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human IL-1RA enables the detection and quantitation of human Interleukin-1RA in human serum, human plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
List Price : 9500.00 USD
AlphaLisa Analyte 2

TAU (human), lyophilized (AlphaLISA)

Human Microtubule-associated Protein Tau (TAU) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human TAU kit, but can be ordered separately.
List Price : 514.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 1449.00 USD - 15700.00 USD

C-peptide (human) AlphaLISA Kit

AlphaLISA® no-wash immunoassay kit for detection of human C-peptide (human connecting peptide) in serum, buffered solution or cell culture medium.
Price Range : 1433.00 USD - 9500.00 USD
AlphaLisa Analyte 2

GDNF (human), lyophilized (AlphaLISA)

Human GDNF (glial cell-derived neurotrophic factor) standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA GDNF detection kits, but can also be ordered separately.
List Price : 240.00 USD
Alpha donor antibody coated

Anti-DIG Alpha Donor beads

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.
Price Range : 467.00 USD - 8500.00 USD
AlphaLISA immunoassay kits

Cry2A AlphaLISA Detection Kit

The AlphaLISA® Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 582.00 USD - 9000.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 855.00 USD - 49600.00 USD
AlphaLisa Analyte 2

ApoB (human), lyophilized (AlphaLISA)

Human ApoB standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ApoB Detection Kit, but can also be ordered separately.
List Price : 287.00 USD
AlphaPlex-545-antibody-bead2

Anti-FLAG AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 570.00 USD - 23400.00 USD
AlphaLisa Analyte 2

IL-18 (human), lyophilized (AlphaLISA)

Human Interleukin 18 (IL18) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL18 kit, but can be ordered separately.
List Price : 240.00 USD

p62 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for p-62 enables the detection and quantitation of human p62 in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
List Price : 12200.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
Price Range : 1449.00 USD - 15700.00 USD
PerkinElmer

AlphaLISA BRD4_BD2 / H4 K8Ac Binding Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain2: BRD4 (BD2) and acetylated histone H4 peptide (H4 K8Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
Price Range : 796.00 USD - 4242.00 USD
AlphaLisa Analyte 2

IgG4 (human), lyophilized (AlphaLISA)

Human IgG4 lyophilized standard for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IgG4 pharmacokinetic kit, but can be ordered separately.
List Price : 233.00 USD
AlphaLISA immunoassay kits

BRCA2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human breast cancer type 2 susceptibility protein (BRCA2) enables the quantitative determination of human BRCA2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

IL-12 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-12 enables the quantitative determination of bovine IL-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 17500.00 USD

AlphaScreen C-MYC Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-c-myc-conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 175.00 USD - 13400.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL1β Detection Kit

The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 571.00 USD - 9700.00 USD

AlphaScreen GST Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 175.00 USD - 13400.00 USD
AlphaLISA assay principle

Cardiac Troponin T (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 18000.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL1β Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL1β Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 571.00 USD - 9700.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 855.00 USD - 49600.00 USD
AlphaLisa Analyte 2

IgA (human), lyophilized (AlphaLISA)

Human IgA standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IgA kit, but can be ordered separately.
List Price : 240.00 USD
AlphaLISA immunoassay kits

IFNγ (cyno) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for monkey cynomolgus interferon gamma (IFNγ) enables the quantitative determination of cynomolgus IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 1590.00 USD - 17200.00 USD
AlphaLISA immunoassay kits

IL-18 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLisa Analyte 2

IL-6 (monkey), lyophilized (AlphaLISA)

Cynomolgus monkey IL-6 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA monkey IL-6 Detection Kit, but can also be ordered separately.
List Price : 287.00 USD
AlphaLISA immunoassay kits

TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 626.00 USD - 9800.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IP10 Detection Kit

The AlphaPlex Samarium (Sm) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 668.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

IgM (human) AlphaLISA Detection Kit

The AlphaLISA® Human IgM Protein Detection Kit is designed for detection and quantitation of human IgM in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 737.00 USD - 6100.00 USD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ Multiplex p-ERK (Eu) (directly coupled) Kit, 50,000 Assay Points

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
List Price : 27600.00 USD
AlphaLisa Analyte 2

IGF1 (human), lyophilized (AlphaLISA)

Human Insulin-like Growth Factor 1 (IGF1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IGF1 kit, but can be ordered separately.
List Price : 240.00 USD

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