PerkinElmer

Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 879 Products and Services

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Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLISA immunoassay kits

hCG (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human chorionic gonadotropin (hCG) enables the quantitative determination of hCG in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 504.00 USD - 15100.00 USD

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 675.00 USD - 12000.00 USD
AlphaLISA immunoassay kits

Claudin 7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 676.00 USD - 19000.00 USD

Anti-Mouse IgM (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgM. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 548.00 USD - 22500.00 USD

Frataxin (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human Frataxin (hFXN) in cell and tissue lysates.
Price Range : 675.00 USD - 9800.00 USD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Tri-Methyl-Histone H3 Lysine 27 (H3K27me3) Cellular Detection Kit

This kit is designed for the detection of tri-methylated Histone H3 Lysine 27 (H3K27me3) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 675.00 USD - 12000.00 USD
AlphaLisa Analyte 2

Claudin 7 (human), lyophilized (AlphaLISA)

Human claudin 7 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Claudin 7 Detection Kit, but can be ordered separately.
List Price : 413.00 USD
AlphaLISA immunoassay kits

Fibrinogen (human) AlphaLISA Detection Kit

The AlphaLISA® Human Fibrinogen Detection Kit is designed for detection and quantitation of Human fibrinogen in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 433.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

Insulin-like Growth Factor 2 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin-like Growth Factor 2 (IGF2) Detection Kit is designed for detection and quantitation of human IGF2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

MMP-3 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Matrix Metalloproteinase-3 (MMP3) Detection Kit is designed for detection and quantitation of human MMP3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP3 and pro-MMP3.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

IgG2b (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG2b antibodies in cell culture media and non-mouse serum.
Price Range : 601.00 USD - 9400.00 USD
alphaimmunoassay1

G-CSF (human) AlphaLISA Detection Kit

The AlphaLISA® Human Granulocyte Colony-Stimulating Factor (G-CSF) Detection Kit is designed for detection and quantitation of human G-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

Price Range : 1476.00 USD - 9800.00 USD
PerkinElmer

AlphaLISA TruHits Kit

Control beads for identifying false positives and interfering compounds in AlphaLISA® no-wash assays.
Price Range : 555.00 USD - 3853.00 USD
AlphaLisa Analyte 2

GM-CSF (mouse), lyophilized (AlphaLISA)

Mouse Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse GM-CSF kit, but can be ordered separately.
List Price : 514.00 USD
AlphaLISA immunoassay kits

Alpha-synuclein (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Alpha-synuclein enables the quantitative determination of Alpha-synuclein in biological fluids such as serum, plasma, cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 626.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

Hyaluronic acid (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.
Price Range : 675.00 USD - 9500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 475.00 USD - 27500.00 USD

AlphaLISA Anti-mono-methyl-Histone H4 Lysine 20 Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against Histone H4 that is mono-methylated at lysine 20 (H4K20me1). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1379.00 USD - 25100.00 USD
AlphaLisa Analyte 2

IgG3 (mouse), lyophilized (AlphaLISA)

Mouse IgG3 lyophilized standard, for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgG3 isotyping kits, but can be ordered separately.
List Price : 231.00 USD
Alpha donor antibody coated

Anti-Rabbit IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-rabbit IgG antibody. This bead can be used to capture rabbit IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 467.00 USD - 8500.00 USD

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 420.00 USD - 9200.00 USD
AlphaLisa Analyte 2

IFNγ (bovine), lyophilized (AlphaLISA)

Bovine IFNγ standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IFNγ kits, but can also be ordered separately.
List Price : 325.00 USD
AlphaLISA immunoassay kits

Fibronectin (human plasma) AlphaLISA Detection Kit

The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
Protein

TSH (human), lyophilized (AlphaLISA)

Human thyroid stimulating hormone (TSH) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human TSH detection kit, but can also be ordered separately.
List Price : 295.00 USD
AlphaLisa Analyte 2

Albumin (mouse), lyophilized (AlphaLISA)

Mouse albumin recombinant standard, for running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse albumin detection kit, but can be ordered separately.
List Price : 514.00 USD
AlphaLisa Analyte 2

Hyaluronic acid (human), lyophilized (AlphaLISA)

Human hyaluronic acid (hHA) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Hylauronic Acid Detection Kit, but can be ordered separately.
List Price : 233.00 USD

Anti-Mouse IgM AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-mouse IgM antibody. The antibody is a monoclonal rat antibody that reacts with the μ heavy chain of mouse IgM. These beads can be used to capture mouse IgM antibodies for AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD

FSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FSH enables the quantitative determination of human follicle stimulating hormone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 504.00 USD - 15100.00 USD

Anti-GST AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-GST antibody. These beads can be used to capture GST-tagged proteins and peptides in AlphaLISA no-wash assays.
Price Range : 571.00 USD - 23400.00 USD
AlphaLISA immunoassay kits

TDP-43 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for TDP-43 is designed for the quantitative determination of Transactive response DNA binding protein 43 (TDP43) in human serum, CSF, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 604.00 USD - 19600.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IFN-γ Detection Kit

The AlphaPlex™ 545 (Terbium) human interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 447.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

IgG3 (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG3 antibodies in cell culture media and non-mouse serum.
Price Range : 675.00 USD - 9400.00 USD
AlphaLISA immunoassay kits

IFNγ (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IFNγ in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 626.00 USD - 10000.00 USD
AlphaLISA immunoassay kits

MMP-9 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of murine MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLisa Analyte 2

Fibrinogen (human), 1 µg, lyophilized (AlphaLISA)

Human fibrinogen standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human fibrinogen Detection Kit, but can be ordered separately.
List Price : 240.00 USD
PerkinElmer

AlphaLISA SureFire Ultra 4EBP1 pT37/46 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-4E-BP1 (Thr37/46) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-4E-BP1 assay kit, but can also be ordered separately.
List Price : 21.00 USD

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 675.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

EGF/EGFR AlphaLISA Binding Kit

The AlphaLISA® EGF/EGFR Binding Kit is designed for the detection of binding activity between Human EGF and EGFR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit the EGF/EGFR interaction.
Price Range : 675.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

LC3B (human) AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of human LC3B in buffered solution or cell lysates. The antibodies in the kit detect both LC3B type I and type II. The analyte in this kit consists of recombinant LC3B fused to His-tag at the N-terminus.
Price Range : 2745.00 USD - 21500.00 USD

CCL5/RANTES (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (CCL5/RANTES) Detection Kit is designed for detection and quantitation of mouse CCL5/RANTES in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

MMP-7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MMP7 enables the detection and quantitation of human pro- and active MMP-7 (hMMP-7) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA immunoassay kits

IFN-γ (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interferon-γ (IFNγ) Detection Kit is designed for detection and quantitation of mouse IFNγ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLisa Analyte 2

TNFα (cynomolgus), lyophilized (AlphaLISA)

Cynomolgus TNFα standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Cynomolgus TNFα Detection Kit, but can also be ordered separately.
List Price : 226.00 USD
AlphaLISA immunoassay kits

IL-33 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 582.00 USD - 14000.00 USD
AlphaLisa Analyte 2

IFN-α (human), lyophylized (AlphaLISA)

Human Interferon-alpha (IFN-α) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The analyte consists of the IFN-α 2b subtype. This standard is already provided in the AlphaLISA Human IFN-α kit, but can be ordered separately.
List Price : 240.00 USD

Anti-V5 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-V5 antibody. The mouse monoclonal antibody detects proteins tagged with the V5 14-amino-acid tag (the 9-amino-acid tag has also been successfully bound by the antibody). 
Price Range : 571.00 USD - 23400.00 USD
AlphaLisa Analyte 2

Biotinylated Human FCGR3A (176Val), lyophilized (AlphaLISA)

Biotinylated human FCGR3A (176Val) receptor. For use in AlphaLISA® no-wash assays.
List Price : 257.00 USD
AlphaLisa Analyte 2

IL-6 (human), lyophilized (AlphaLISA)

Human Interleukin-6 (IL6) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL-6 kit, but can be ordered separately.
List Price : 240.00 USD

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