PerkinElmer

3D Cell Culture & Analysis

OF

3D cell cultures, microtissues, and organoids are increasingly being used to bridge the gap between 2D cell cultures and in vivo animal models. 3D cell models are more physiologically relevant than biochemical assays and 2D cell cultures, as they more closely represent the microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. 3D cell models show a higher degree of morphological and functional differentiation – again, similar to in vivo cell characteristics.

Grow, detect, and analyze 3D cell cultures with our best-in-class solutions, and begin generating more physiologically relevant data that can power better-informed decisions.

3D-Cell-Culture-4
3D-Cell-Culture-4

Grow 3D cell culture with speciality microplates

Choose from a range of carefully designed microplate technologies that produce uniform 3D cultures – time after time. We offer a variety of microplates and advanced surface coatings to meet the needs of unique cell types and applications. 
  • PerkinElmer CellCarrier Spheroid Ultra Low Attachment Microplates – Get consistent 3D cellular spheroid formation with minimal cell-plate adhesion, promoting single spheroid formation with robust circular formation. The optically-clear, round bottom produces high-quality imaging results.
  • Insphero GravityTRAP™ ULA Plate – Ideal for long-term cultivation, the SureXchange™ tapered ledge facilitates easy, medium exchange and prevents microtissue loss. The flat-bottom observation chamber enables brightfield microscopy applications and size measurements.
  • GravityPLUS™ Plate - A hanging drop plate for outstanding size consistency, featuring SureDrop™ microtechnology, with variation in diameter of 5% or less across an entire 96-well plate. Hanging drop spheroid formation is recommended for co-culture and primary cells.
  • Insphero GravityPLUS™ Hanging-Drop Spheroid Culture System - A combination of the GravityPLUS™ Plate (formulation of spheroids in a hanging drop plate), and the GravityTRAP™ ULA Plate (for the capture and assay of cells).
3D InSight™ Human Liver Microtissues from InSphero labeled imaged on the Operetta CLS system
3D InSight™ Human Liver Microtissues from InSphero labeled imaged on the Operetta CLS system

Detect with sensitive detection and high-content imaging solutions

Our multimode microplate readers and high-content analysis systems, plus innovative reagents, provide you with highly sensitive solutions for 3D cell culture imaging and detection.
  • Opera Phenix™ high-content screening system is the premier confocal solution for 3D high-content screening, enabling fast multichannel 3D acquisition at high image quality.
  • With its unique combination of technologies, the Operetta CLS™ high-content analysis system provides flexibility, sensitivity, and resolution for 3D imaging.
  • With the EnSight multimode plate reader you can monitor the growth and health of your 3D cell cultures by measuring size, shape, and more using image cytometry. Combine imaging with other technologies such as AlphaLISA® to detect secreted proteins.
  • ATPlite™ 3D and ATPlite 1step 3D reagent kits for cell viability and proliferation provide a simple, robust protocol for ATP-content endpoint measurements of 3D spheroids.
3D-Cell-Culture-6
3D-Cell-Culture-6

Analyze with powerful and intuitive informatics solutions to uncover new insights

Our scientific software and informatics solutions give you the tools to optimize imaging of 3D cell models and then aggregate, search, mine, and visualize critical data - and turn it into actionable insights.
  • Turn data into understanding with the intuitive Harmony™ imaging and analysis software for the Opera Phenix and Operetta CLS systems. You can accelerate 3D image acquisition and analysis, better understand spatial relationships, discover new perspectives not visible in 2D and measure volume and morphology in 3D. Optional PreciScan™ intelligent acquisition lets you pre-scan to locate your sample in the well for significantly reduced acquisition and analysis times.
  • Manage the large amounts of data that high-content analysis generates with Columbus™ image data storage and analysis software. Add cluster computing capabilities for even greater power when acquiring large datasets for working with 3D samples.
Business Insights (6)
Filters:

1-6 of 6 Business Insights

Sort by

  • Application Note

    Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System

    Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.

  • Application Note

    Phenotypic Profiling of Autophagy using the Opera Phenix High-Content Screening System

    Cells constantly sense their environment and their response is a spatio-temporal summation of all signals. To maintain physiological stability, cells need to adjust to environmental changes, a process called homeostasis. One of the most important processes involved in maintaining homeostasis is autophagy, and its significance was recognized by the award of the Nobel Prize for Physiology in 2016 to Yoshinori Ohsumi for the discovery of its underlying mechanisms. Although this is not fully understood, it is believed that autophagy can prevent tumor development by degrading, for example, damaged organelles and protein aggregates.

  • Application Note

    Measuring FRET using the Opera Phenix High-Content Screening System

    Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, including cancer. Therefore, inhibiting protein-protein interactions represents an emerging area in drug design.

  • Application Note

    Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

    Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS™ high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony® software contribute to the robustness of the high-content assay.

  • Application Note

    Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

    Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

  • Case Study

    High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis

    One of the greatest challenges in multiple sclerosis (MS) therapy is the halting or reversal of the failure of remyelination in the brain in order to reverse disabilities in MS patients. This case study highlights the recent work of Dr. Paul Tesar and colleagues at the Case Western Reserve University School of Medicine, which could potentially lead to such novel treatments, as it aims to control the function of stem cells in the body and thereby to help the body repair itself.

Products & Services (9)
Filters:

1-9 of 9 Products & Services

Sort by

  • 1000x1000_Opera_Phenix

    Opera Phenix High-Content Screening System

    The Opera Phenix High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® High Content Screening System, the Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and microtissues.
  • 1000x1000_Operetta

    Operetta CLS High-Content Analysis System

    Uncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS high-content analysis system. Featuring a unique combination of technologies, the system delivers all the speed, sensitivity and resolution you need to reveal fine sub-cellular details. And with our simple, powerful Harmony 4.5 software, Operetta CLS lets you find even subtle phenotypic changes.
  • EnSight Multimode Plate Reader

    EnSight Multimode Plate Reader

    The EnSight multimode plate reader combines labeled & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.
  • ATPlite 3D Kit

    ATPlite 3D, 300 assay point kit

    ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids.
    List Price : 149.00 USD
  • ATPlite 1-step 3D kit

    ATPlite 1Step 3D, 10 mL kit

    ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. The 1-step format only requires one addition step, and can be used for continuous processing.
    List Price : 76.00 USD
  • CellCarrier Spheroid ULA 96-well Microplates

    Round bottom, clear 96-well polystyrene microplates coated with Ultra Low Attachment (ULA) surface for 3D culture of mammalian cells. This ULA coating in round-bottom wells facilitates formation of round spheroids after incubating seeded cells.
    Price Range : 254.00 USD - 1068.00 USD
  • Columbus Image Data Storage and Analysis System

    Columbus Image Data Storage and Analysis System

    Our Columbus Image Data Storage and Analysis system is an instrument agnostic image analysis and management platform. The Columbus system is the only system that provides universal high-volume image data storage and analysis and brings access to images from a wide range of sources including all major high content screening instruments.
  • Harmony Software 1

    Harmony High-Content Imaging and Analysis Software

    Easily quantify complex cellular phenotypes with Harmony high-content analysis software. Harmony software is designed for Operetta CLS and Opera Phenix high-content screening systems.

    Coming soon!
    Our forthcoming release includes an updated version of PreciScan, which significantly reduces imaging times and data volumes for 3D cell models grown in hydrogels.

  • PreciScan intelligent acquisition software lets you first pre-scan at low magnification

    PreciScan Intelligent Acquisition

    Harness the power of intelligent image acquisition with PreciScan for more efficient high-content imaging and analysis. This optional plug-in for Harmony high-content analysis software enables you to more accurately target your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.