3D Cell Culture & Analysis


The rising adoption of 3D cell culture has been pivotal in establishing more biologically relevant morphological and functional models that are key to understanding tissue morphogenesis, disease mechanisms, drug discovery, and tissue engineering.

PerkinElmer offers a full product portfolio for 3D applications that includes spheroid culture platforms, reagents for metabolic analysis, and premier High Content Analysis and Screening imaging platforms.


Form 3D Spheroids with PerkinElmer Plates


Cells in 3D spheroids mimic in vivo functionality within an in vitro environment by promoting biomimetic cell-to-cell interactions that more reflect the functional complexity of native tissue. PerkinElmer offers two spheroid culture platforms to meet the needs of unique cell types and applications

  • PerkinElmer CellCarrier Spheroid Ultra Low Attachment Microplates – Get consistent 3D cellular spheroid formation with minimal cell-plate adhesion, promoting single spheroid formation with robust circular formation. The optically-clear, round bottom produces high-quality imaging results.
  • Insphero GravityTRAP™ ULA Plate – Ideal for long-term cultivation, the SureXchange™ tapered ledge facilitates easy, medium exchange and prevents microtissue loss. The flat-bottom observation chamber enables brightfield microscopy applications and size measurements.
  • GravityPLUS Plate - A hanging drop plate for outstanding size consistency, featuring SureDrop™ microtechnology, with variation in diameter of 5% or less across an entire 96-well plate. Hanging drop spheroid formation is recommended for co-culture and primary cells.
  • Insphero GravityPLUS™ Hanging Drop Spheroid Culture System - A combination of the GravityPLUS™ Plate (formulation of spheroids in a hanging drop plate), and the GravityTRAP™ ULA Plate (for the capture and assay of cells).

Monitor cell activity with our broad portfolio of reagents

Understand cell viability, proliferation, cytotoxicity, and metabolism of your 3D spheroids with PerkinElmer’s diverse selection of radiochemicals, reagents, consumables, and detection platforms.

PerkinElmer in vivo near infrared (NIR) agents are suitable for imaging microtissues on high content screening platforms, such as the Opera Phenix and Operetta CLS, although they were originally designed to monitor and quantify biological events such as cancer or inflammatory diseases in small animals. These NIR agents are composed of two parts: a biological part that mediates the interaction of the target molecule or gets cleaved by a target protein; and near-infrared dye with an emission wavelength of 700 nm, which visualizes the biological event. Light absorption and scattering in biological tissue reaches a minimum at wavelengths between 600-900 nm, thus these dyes are especially well-suited for imaging in microtissue models.


High-Content Screening & Analysis Systems for 3D Cell Cultures

Designed with 3D cell cultures in mind, our high-content screening systems let you quickly and easily generate content-rich, physiologically relevant data. Powerful technologies such as confocal spinning-disk imaging and high NA water immersion objectives ensure the light penetrates the sample and enable you to acquire high resolution image stacks at different levels within the sample.

You can then turn that data into understanding with our intuitive Harmony™ software, and options such as PreciScan™ intelligent acquisition so you can pre-scan to locate your sample in the well for significantly reduced acquisition and analysis times.

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"3D Cell Culture & Analysis" 1-11 of 11 Products and Services


Operetta High-Content Imaging System

As scientists grapple with increasingly complex biological questions, they need to conduct larger, more complex imaging experiments. So they’re turning to the Operetta® High Content Imaging System – the ideal solution for analyzing the inner workings of healthy and diseased cells.

Operetta CLS High-Content Analysis System

Uncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS™ high-content analysis system. Featuring a unique combination of technologies, the system delivers all the speed, sensitivity and resolution you need to reveal fine sub-cellular details. And with our simple, powerful Harmony 4.5 software, Operetta CLS™ lets you find even subtle phenotypic changes.

Opera Phenix High-Content Screening System

The Opera Phenix™ High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® High Content Screening System, the Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and microtissues.

ATPlite 3D, 300 assay point kit

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids.
List Price : 139.00 USD

ATPlite 1Step 3D, 10 mL kit

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. The 1-step format only requires one addition step, and can be used for continuous processing.
List Price : 72.00 USD

CellCarrier Spheroid ULA 96-well Microplates

Round bottom, clear 96-well polystyrene microplates coated with Ultra Low Attachment (ULA) surface for 3D culture of mammalian cells. This ULA coating in round-bottom wells facilitates formation of round spheroids after incubating seeded cells.
Price Range : 235.00 USD - 988.00 USD
Gravity Plus Hanging Drop System

GravityPLUS™ Hanging Drop System 96-well

The InSphero GravityPLUS™ Hanging Drop System is a hanging drop two-piece microplate for the generation, long-term cultivation, observation and testing of scaffold-free 3D microtissue spheroids in 96-well format.
Price Range : 482.00 USD - 1444.00 USD
GravityTrap ULA

GravityTRAP™ ULA Plate 96-well, pack of 10

The InSphero GravityTRAP™ Ultra-Low Attachment (ULA) microplate represents a simple, flexible, and automation-compatible platform for the generation, long-term cultivation, observation and testing of scaffold-free 3D microtissue spheroids in 96-well format. Each plate consists of a special non-adhesively coated 96-well sterile-packaged GravityTRAP™ (Tissue Re-aggregation and Assay Plate), and lid.
List Price : 315.00 USD
Gravity Plus

GravityPLUS™ Plate 96-well, pack of 10

The InSphero GravityPLUS™ Plate is a microtissue production plate used in the generation of scaffold-free 3D microtissue spheroids in 96-well format. This plate is also used as the upper plate of the GravityPLUS™ Hanging Drop System.
List Price : 1221.00 USD
PreciScan intelligent acquisition software lets you first pre-scan at low magnification

PreciScan Intelligent Acquistion

Harness the power of intelligent image acquisition with PreciScan for more efficient high-content imaging and analysis. This optional plug-in for Harmony high-content analysis software enables you to more accurately target your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.
Harmony Software 1

Harmony High-Content Imaging and Analysis Software

Only the powerfully simple Harmony® High Content Imaging and Analysis Software unleashes the full potential of your Operetta® High Content Imaging System or Opera Phenix High Content Screening System.

"3D Cell Culture & Analysis" 1-11 of 11 Products and Services