Tyramide Signal Amplification (TSA) Systems

2012 Wellcome Image Award Winners

Increase Imaging Detection 100X with Tyramide Signal Amplification (TSA)!

Amplify Your IHC, IF or ISH Signal with TSA Reagent Solutions ... View TSA Image Gallery

Why Multiplex?!

Why Multiplex?

Learn how TSA dramatically expands options for Multiplex detection ... View Rapid Learning Presentation

Signal Amplification for Immunohistochemistry

Signal Amplification for Immunohistochemistry

Improve the sensitivity of detection by 100-fold or more ... View Rapid Learning Presentation

Signal Amplification for In Situ Hybridization

Signal Amplification for In Situ Hybridization

Get higher sensitivity with minimal change to your existing protocol ... View Rapid Learning Presentation

TSA Published Papers

TSA Published Papers

View peer-reviewed scientific literature published by researchers using TSA solutions ... Read More

  • Increase Imaging Detection 100X with Tyramide Signal Amplification (TSA)!
  • Why Multiplex?
  • Signal Amplification for Immunohistochemistry
  • Signal Amplification for In Situ Hybridization
  • TSA Published Papers

How Can TSA Benefit Your Research?

Tyramide Signal Amplification (TSA™) technology enables reliable detection and quantitation of proteins and nucleic acids that often cannot be measured with standard methods.  TSA reagent kits can improve your current Immunohistochemistry, (IHC), immunofluorescence (IF) or  in situ hybridization (ISH) protocol using your existing imaging hardware, ANY microscope, ANY sample. View our two rapid learning presentations on TSA with in situ hybridization and Immunohistochemistry & Immunocytochemistry to learn more.  

Learn how to add TSA to your current protocol. 

TSA signal molecules are covalently bound to tyrosine in proteins immediately proximal to the target.  This assures outstanding resolution and stability for highly multiplexed assays.  

Assessment of tissue microenvironment for multiple markers at diverse expression levels is critical for advancing research in areas like cancer, neuroscience and developmental biology.    Technologies for early detection of low abundance analytes in tissue can help move your research forward faster. Track early developmental changes in progenitor cells or changes in tissue microenvironment as disease indicators. Meet your tissue analysis & microscopy challenges without changes to your current imaging platform with TSA Reagent Kits.

  • Detect & quantify low abundance analytes
  • Improve performance of weakly binding primary antibodies
  • Use less antibody & probe for improved background with reduced cost 
  • Reduce scanning time producing images faster
TSA detection verses Standard detection2
Prostate Specific Antigen (PSA) in Normal Prostate Tissue, FFPE
Sections.   Image Source: PerkinElmer Technical Support.


TSA Selection Guide

TSA Plus Kits
TSA Plus provides ~20X improved sensitivity over standard tyramide reagents.
TSA Plus Kits for direct fluorescence detection
ExcitationEmissionIncludesAdd
TSA Plus Fluorescein494 nm517 nm
Plus Amplification
Reagent, Plus
Amplification Diluent
TSA Plus TMR550 nm570 nm
TSA Plus Cyanine 3550 nm570 nm
TSA Plus Cyanine 3.5581 nm596 nm
TSA Plus Cyanine 5648 nm667 nm
TSA Plus Cyanine 5.5673 nm692 nm
TSA Plus Kits for chromogenic or indirect fluorescence detection
HaptenIncludesAdd
TSA Plus BiotinBiotin
Plus Amplification
Reagent, Plus
Amplification Diluent
TSA Plus DIGDigoxigenin
TSA Plus FluoresceinFluorescein
TSA Plus DNP2,4 Dinitrophenyl
Plus Amplification
Reagent, Plus
Amplification Diluent
anti-DNP conjugate
blocking reagent
TSA Kits, original formulation
TSA Systems for direct fluorescence detection
ExcitationEmissionIncludesAdd
TSA Coumarin402 nm443 nm

Amplification Reagent, Amplification Diluent, Blocking Reagent, Streptavidin-HRP

TSA Fluorescein494 nm517 nm
TSA TMR550 nm570 nm
TSA Cyanine 3550 nm570 nm
TSA Cyanine 5648 nm667 nm
TSA Systems for chromogenic or indirect fluorescence detection
HaptenIncludesAdd
TSA BiotinBiotinAmplification Reagent, Amplification Diluent, Blocking Reagent, Streptavidin-HRP
TSA FluoresceinFluorescein


Adding TSA to Your Protocol

ISH protocol for TSA video

View Video: Adding TSA to Your ISH protocol
ISH (in situ hybridization) : Probes may be labeled with biotin, DIG, fluorescein or other haptens. 

ICH protocol for TSA video

View Video: Adding TSA to Your IHC protocol
IHC (Immunohistochemistry) and ICC, (immunocytochemistry): HRP is typically introduced as a secondary antibody or streptavidin conjugate. 

By adding TSA to your in situ hybridization, immunocytochemistry assay or immunofluorescence protocol, you will:

Achieve outstanding resolution
TSA binds covalently to proteins immediately proximal to the target, making it ideal for sub-cellular localization of proteins and nucleic acids of interest.

Improve results with minimal change to our workflow
Use TSA to amplify any assay where horseradish peroxidase (HRP) can be introduced. Signal amplification occurs during a simple 10-minute room temperature incubation.

Eliminate background problems and improve specificity of detection
Use Use 10- to 100-fold less primary antibody or probe for each slide after adding TSA to your protocol in most cases.  Higher dilution of primary detection reagents reduces background and improves the specificity of your assay. 

Investigate co-localization more effectively
Stable covalent labeling with TSA provides many more options for multiplexed detection within a single tissue section, including the possibility of using more than 1 primary antibody from the same species. View more multiplex detection images in the TSA Image Gallery.
Simultaneous detection of germ cell marker (red), Leydig cell marker (green), and smooth muscle marker (blue) in fetal rat testis using TSA Plus direct fluorescence 2
Contact us for more information on TSA, or to talk to your local representative.  


Products

HCA ImagAmp™ Reagent Kits For High Content Screening and Analysis

Now you can measure what was previously undetectable in your high content screening (HCS) immunofluorescence...  View more

Horseradish Peroxidase Labeled Reagents

Horseradish peroxidase (HRP) is a 44 kDa glycoprotein that catalyzes the oxidation of specific substrates...  View more

Multi-Fluor Combination Kits

TSA Plus Fluorescence SystemsSignal amplification for IHC, ISH and related applicationsDirect fluorescence...  View more

Opal Multiplex Tissue Staining

Breast Cancer TIL'sCD4, CD8, CD20, cytokeratin, DAPIBreast CancerER, PR, Her2, Ki-67, cytokeratin, DAPIMelanoma...  View more

TSA Plus Evaluation Kits

TSA Plus Evaluation Kits allow rapid testing of signal amplification in your assay. Up to 1000-fold...  View more

TSA Plus Systems

Open your eyes to the advantages of TSA PlusTyramide Signal Amplification Plus (TSA Plus) kits offer...  View more

TSA Reagent Packs

Tyramide Signal Amplification (TSA™) is a patented technology that amplifies both chromogenic and fluorescent...  View more