How Can TSA Benefit Your Research?
Tyramide Signal Amplification (TSA™) technology enables reliable detection and quantitation of proteins and nucleic acids that often cannot be measured with standard methods. TSA reagent kits can improve your current Immunohistochemistry, (IHC), immunofluorescence (IF) or in situ hybridization (ISH) protocol using your existing imaging hardware, ANY microscope, ANY sample. View our two rapid learning presentations on TSA with in situ hybridization and Immunohistochemistry & Immunocytochemistry to learn more.
Learn how to add TSA to your current protocol.
TSA signal molecules are covalently bound to tyrosine in proteins immediately proximal to the target. This assures outstanding resolution and stability for highly multiplexed assays.
Assessment of tissue microenvironment for multiple markers at diverse expression levels is critical for advancing research in areas like cancer, neuroscience and developmental biology. Technologies for early detection of low abundance analytes in tissue can help move your research forward faster. Track early developmental changes in progenitor cells or changes in tissue microenvironment as disease indicators. Meet your tissue analysis & microscopy challenges without changes to your current imaging platform with TSA Reagent Kits.
- Detect & quantify low abundance analytes
- Improve performance of weakly binding primary antibodies
- Use less antibody & probe for improved background with reduced cost
- Reduce scanning time producing images faster
Prostate Specific Antigen (PSA) in Normal Prostate Tissue, FFPE
Sections. Image Source: PerkinElmer Technical Support.
TSA Selection Guide
|TSA Kits, original formulation|
|TSA Systems for direct fluorescence detection|
|TSA Coumarin||402 nm||443 nm|
Amplification Reagent, Amplification Diluent, Blocking Reagent, Streptavidin-HRP
|TSA Fluorescein||494 nm||517 nm|
|TSA TMR||550 nm||570 nm|
|TSA Cyanine 3||550 nm||570 nm|
|TSA Cyanine 5||648 nm||667 nm|
View Video: Adding TSA to Your ISH protocol
ISH (in situ hybridization) : Probes may be labeled with biotin, DIG, fluorescein or other haptens.
View Video: Adding TSA to Your IHC protocol
IHC (Immunohistochemistry) and ICC, (immunocytochemistry): HRP is typically introduced as a secondary antibody or streptavidin conjugate.
By adding TSA to your in situ hybridization, immunocytochemistry assay or immunofluorescence protocol, you will:
Achieve outstanding resolution
TSA binds covalently to proteins immediately proximal to the target, making it ideal for sub-cellular localization of proteins and nucleic acids of interest.
Improve results with minimal change to our workflow
Use TSA to amplify any assay where horseradish peroxidase (HRP) can be introduced. Signal amplification occurs during a simple 10-minute room temperature incubation.
Eliminate background problems and improve specificity of detection
Use Use 10- to 100-fold less primary antibody or probe for each slide after adding TSA to your protocol in most cases. Higher dilution of primary detection reagents reduces background and improves the specificity of your assay.
Investigate co-localization more effectively
Stable covalent labeling with TSA provides many more options for multiplexed detection within a single tissue section, including the possibility of using more than 1 primary antibody from the same species. View more multiplex detection images in the TSA Image Gallery
for more information on TSA, or to talk to your local representative.
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