AlphaLISA Kits for Biomarkers and Cytokines

Eliminate ELISA limitations - convert to AlphaLISA!

Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µl) to conserve precious sample.

   
AngiogenesisEPOTNFαTNFα (mouse)TNFα (bovine)
VEGFBVEGFCVEGFDVEGF
VEGFA (mouse)
AutophagyAlphaLISA p62 kitLC3B
Biologics/
Bioprocess
Albumin (human)CHO-PCHO-P broad reactivityE. coli HCP
IgAIgEIgGIgG1 (pharmacokinetic)
IgG4 (pharmacokinetic)IgMPEG-IgMNSO-P
PER.C6Residual Protein AIgG1 (isotyping)IgG2 (isotyping)
IgG3 (isotyping)IgG4 (isotyping)Albumin (mouse)IgA (mouse)
IgE (mouse)Total IgG (mouse)IgG1 (mouse)IgG2a (mouse)
IgG2b (mouse)IgG3 (mouse)IgM (mouse)IgE (anti-mouse)
IgG1 (anti-mouse)IgG2a (anti-mouse)IgG2b (anti-mouse)IgG3 (anti-mouse)
IgM (anti-mouse)IgA (anti-bovine)IgG1 (anti-bovine)IgG2 (anti-bovine)
IgM (anti-bovine)Total IgG (anti-bovine)
CancerAFPCA125Caspase-3 activeCXCL11/I-TAC
EPOERBB2/HER2HGFR/c-METIFN-β
MMP1MMP2MMP3MMP9
MMP9 (mouse)MMP13 (human)β-NGFPSA
TNFαTFF3TIMP1TNFα (mouse)
TNFα (bovine)
CardiovascularAlpha-2 macroglobulinCardiac Troponin ID-dimerEPO
ICAM-1MyeloperoxidaseMyoglobinPAI-1
PCSK9PlasminogenRenin/ProrenintPA
Transferrin


Central Nervous SystemAmyloid β 1-15Amyloid β 1-40Amyloid β 1-40 (high specificity)Amyloid ß 1-40 (mouse/rat)
Amyloid β 1-42Amyloid β 1-42 (high specificity)Amyloid β 1-42 (mouse/rat)Amyloid β 1-x
Amyloid β oligomersBDNFEPOGDNF
sAPPα (C-term specific)Tau
InflammationCXCL1/GRO-αCCL2/MCP1CCL2/MCP1 (mouse/rat)CCL3/MIP-1α
CCL4/MIP-1βCCL5 / RANTESCCL5 / RANTES (mouse)CRP
CXCL9/MIGCXCL10/IP-10G-CSFGM-CSF
GM-CSF (mouse)IFN-αIFN-γIFN-γ (mouse)
IL1αIL1α (bovine)IL1βIL1β (mouse)
IL2IL2 (mouse)IL3IL4
IL5IL6IL6 (mouse)IL7
IL7 (mouse)IL8IL10IL10 (mouse)
IL11IL1β (rat)IL12 (p70)IL13
IL15IL15 (mouse)IL17IL17A (mouse/rat)
IL17A (mouse/rat - serum)IL18IP-10TNFα
IL22 (human/rat)TNFα (mouse)TNFα (bovine)
MetabolicAdiponectinAdiponectin (mouse)Albumin (human)Albumin (mouse)
CortisolC-peptide (mouse/rat)FGF21GLP-1
GlucagonGrowth HormoneIGF1IGF2
InsulinLeptinLeptin (mouse)Prolactin
VirologyHIV p24 (high sensitivity)HIV p24

Many Benefits of Alpha Technology

You have several options to realize the benefits of AlphaLISA:

  • AlphaLISA kits for specific biomarker and cytokine analytes
  • AlphaLISA Toolbox for constructing your own assays
  • OnPoint Reagent Services in which PerkinElmer develops, optimizes and transfers your custom biomarker and cytokine assay for you

Learn more about OnPoint Reagent Services

References:

A Luminescent Oxygen Channeling Immunoassay for the Determination of Insulin in Human Plasma
Fritz Poulsen and Kirsten Borres Jensen, J Biomol Screen OnlineFirst, published on January 26, 2007

Conversion of ELISA to No Wash Technologies
James R. Beasley, Robert Swanson, Jingchun Yang, and David Dunn. Pharmacopeia Drug Discovery

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Easy automation for a broad range of analytes

AlphaLISA is preferable to ELISAs for a wide range of applications. Easy to automate and miniaturize, AlphaLISA assays are perfect for analytes requiring a high-throughput format or ultra-sensitivity and a wide dynamic range. The reliable no-wash technology saves your lab time and money.

AlphaLISA immunoassay kits

Each AlphaLISA immunoassay kit has five components:

  • AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
  • Streptavidin-coated Donor beads
  • Biotinylated anti-analyte antibody #2
  • Lyophilized analyte
  • AlphaLISA Immunoassay buffer (10x)

The kits can be used in a 96-, 384- or 1536-well format.


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Products

Angiogenesis

Convert a tedious ELISA to a faster, more reproducible alternative- PerkinElmer's highly sensitive, ...  View more

Autophagy

As a primary route for turnover of stable and defective cellular proteins, autophagy is a process critical...  View more

Biologics / Bioprocess

Shorten the time it takes you to quantitate monoclonal antibodies (IgG1 and IgG4, IgA, IgE, IgG, IgM), detect...  View more

Cancer

Shorten the time it takes you to run cancer biomarker assays to less than 3 hours and benefit from small...  View more

Cardiovascular

Measure more cardiovascular biomarkers and analytes faster with a more reproducible alternative to ELISA-...  View more

Central Nervous System

Central nervous system (CNS) research faces the same hurdles as many other therapeutic areas – the need...  View more

Inflammation

PerkinElmer’s homogeneous AlphaLISA® inflammation biomarker assays are a user-friendly and versatile...  View more

Metabolic

Measure more metabolic biomarkers FASTER. Convert a tedious ELISA to a faster, more reproducible alternative-...  View more

Virology

Shorten the time it takes you to run biomarker assays to less than 3 hours and benefit from small sample...  View more