Harnessing the power of fluorescence for in vivo imaging
The phenomenon of fluorescence is utilized to obtain biological information within many technologies used on the path from gene to clinic. Successful development of fluorescence agents for in vivo imaging depends on an understanding of biochemistry, cell biology and physiology, combined with knowledge of how to apply optimized chemistries. By leveraging in-house expertise and collaborating with key academic and pharmaceutical partners, PerkinElmer integrates chemistry, biology, and pharmacology to drive the development of fluorescence agents from initial concepts into validated products and applications. This approach enables key pharmacokinetic and delivery barriers in vivo to be overcome while taking advantage of biological and chemical amplification strategies whenever possible to optimize performance.
Optimizing application-specific performance
PerkinElmer is developing an expanding range of fluorescence agents, with different mechanisms of action, to selectively highlight in vivo the biological targets, biomarkers and pathways that underlie disease progression and therapeutic response.
For in vivo imaging, agents must have high affinity and specificity for their intended target and overcome the numerous "biological hurdles" present in complex living organisms, such as rapid excretion, non-specific accumulation in non-target tissues, metabolism, clearance and pharmacologic delivery barriers. Even in animal models, biocompatibility and toxicity profiles are critical to enable a truly non-invasive assessment of biological activity and therapeutic response. Equally important, is to develop the imaging agent with a mechanism of action best suited for the intended application and one which yields the greatest amount of information.
- Biological, functional and physiological information
- Easily applied to standard animal models
- use together for simultaneous monitoring of multiple molecular processes
- custom-design a fluorescence agent using PerkinElmer tags and/or nanoparticles
- Designed and optimized for translational in vivo imaging narrow spectrally-separated excitation/emission spectra in the NIR region
- high fluorescence efficiency (quantum yield)
- biocompatible, biodegradable and excretable
- chemical and photo-stability in vivo
- Compatible with a variety of in vivo imaging systems
- Developed and optimized for use with systems based on FMT technology