Western Blotting

Western blotting (also known as western immunoblotting or immunoblot) is the most widely used technique for detecting specific proteins in complex samples like cell or tissue lysates, cell culture supernatants or body fluids. The combination of size-based separation with SDS-PAGE and antibody-based detection provides outstanding specificity. Because of the high specificity of its results, western blotting is often used to confirm other protein detection methods like ELISA and RIA. 

Western blotting can quantitatively assess full-length expression products, including post-translational modifications. This makes it a valuable tool for clone selection in development of biotherapeutics as well as basic research. 

Resources for western blotting

Andrews Lab Quantitative Western Blotting Protocol
Western Blot Wikipedia Page

Western blotting is a multi-step process, and PerkinElmer provides a variety of tools to ensure reliable results and keep your research on track.

1wb.jpgProtein Markers
2wb.jpgLightning Blot Fast Transfer System NEW!
PolyScreen PVDF Transfer Membranes
Protran® Nitrocellulose transfer
3wb.jpgHRP Conjugates
Radiometric Blotting Detection Reagents
AP Conjugates
Labeled Streptavidin
Biotin Labeled Secondary Antibodies
4wb.jpgWestern Lightning Chemiluminescent HRP Substrates
Radiometric Blotting Detection Reagents
Chromogenic Blotting Substrates
CDP-Star® Chemiluminescent AP Substrates
5wb.jpgCyclone Plus Phosphor Imager

Products

Alkaline Phosphatase Labeled Reagents

Alkaline phosphatase (AP) is a 140 kDa dimeric metalloenzyme that catalyzes the removal of phosphate....  View more

Biotin Labeled Secondary Antibodies

Biotin-labeled affinity purified antibodies offer excellent selectivity for ELISA, western blotting,...  View more

CDP-Star® Reagents for Detection of Alkaline Phosphatase (AP)

CDP-Star® chemiluminescence reagents provide outstanding sensitivity, continuous, strong signal for ...  View more

Chromogenic Substrates for Detection of HRP and AP

Chromogenic detection gives the convenience of direct colorimetric visualization of results without ...  View more

Horseradish Peroxidase Labeled Reagents

Horseradish peroxidase (HRP) is a 44 kDa glycoprotein that catalyzes the oxidation of specific substrates...  View more

Labeled Streptavidin

PerkinElmer offers a variety of labeled streptavidin reagents for detection of biotin labels in a broad...  View more

Lightning Blot™ System

Better Westerns, Faster The Lightning Blot System delivers high quality protein transfers across a broad...  View more

Protein Molecular Weight Markers

Protein molecular weight markers (or protein ladders) are used in SDS-PAGE (sodium dodecyl sulfate polyacrylamide...  View more

Radiometric Blotting Detection Reagents

125I labeled reagents offer highly specific and sensitive direct autoradiographic detection of membrane-bound...  View more

Transfer Membranes for Western Blotting

PerkinElmer offers only the highest quality transfer membranes. They facilitate detection by concentrating...  View more

Western Lightning Chemiluminescent HRP Substrates for Western Blotting

Enhanced chemiluminescence (ECL) with horseradish peroxidase (HRP) is the most referenced detection ...  View more