Calcium Photoproteins-Aequorin & Photina
Choose from AequoScreen aequorin cell lines, AequoZen frozen cells or PhotoScreen Photina® cell lines.
We bring you the best performing biological coupling by providing the choice of two different photoproteins to measure calcium signaling, resulting in large signal-to-noise ratios, low background levels and high Z' short incubation for tested compounds, with easy to follow reaction kinetics.The simpler and faster assay protocol, compared to conventional fluorescence intracellular calcium assays, enables faster results in addition to reducing reagent costs. The ability to now work with autofluorescent compounds reduces the number of false positives from screens.
Optimal localization of a Ca2+-activated photoprotein within the mitochondria amplifies the luminscent response triggered by GPCRs coupled to the Gq/PLC pathway. These photoproteins deliver high signal-to-background ratios, high throughput (over 200,000 data points per day, high Z' values with low CVs and low solvent resistance.
Some of these luminescent cell lines are also intense enough for use on dual mode fluorescence / luminescence readers. Visit the cell lines, frozen cells & membranes product listing to understand the validation for each product.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled...
The aequorin plasmid, which expresses the aequorin protein under the control of the CMV promoter, has...
AequoZen: Frozen cells with Aequorin for calcium testing. Using Aequorin for calcium assays is much ...
PhotoScreen (Photina®) photoproteins are a kinetic luminescence technology for measuring calcium flux...