AlphaLISA Epigenetic Tool Box

Highly sensitive no wash detection for measuring peptide and protein modification by epigenetic enzymes.

Use convenient, versatile AlphaLISA technology to detect specific methylation and acetylation states for epigenetics research. In addition to providing high-quality data and robust performance, AlphaLISA assays are simple and quick to optimize. Easy to automate and miniaturize, AlphaLISA are fully validated for mark specificity and cross reactivity.

AlphaLISA technology is a no-wash homogeneous proximity immunoassay that includes Alpha Donor and AlphaLISA Acceptor beads to quantitate activity on histone, peptide, or protein substrates. In vitro methylation or acetylation of substrate is performed by incubating enzyme (for example, a histone acetyltransferase or methyltransferase) with S-adenosylmethionine (SAM) or Acetyl CoA cofactor and histone substrate. Following the enzymatic reaction, methylation or acetylation is monitored using mark-specific anti-methyl or anti-acetyl antibody-conjugated AlphaLISA Acceptor beads. Once streptavidin-coated Donor beads are added to capture the substrate, the beads are brought into close proximity. The Donor beads are excited by a laser at 680 nm to release singlet oxygen. The singlet oxygen causes a reaction in the Acceptor bead and light emission is produced at 615 nm. This emission is proportional to the amount of methylated or acetylated substrate present.



AlphaLISA
Acetyl Modifications
ReagentsValidation: Technical Data Sheets
TargetMark SpecificityAcetyltransferasesDeacetylases
Histone H3Unmodified (H3K4)SIRT1
Unmodified H3K9/K27HDAC1
H3K9acp300
H3K27acHDAC1
Histone H4H4Kac panTIP60
p53K382acSIRT1
TauO-GlcNAcOGA
Methyl Modifications
ReagentsValidation: Technical Data Sheets
TargetMark SpecificityMethyltransferasesDemethylases
Histone H3Unmodified (H3K4)LSD1
Unmodified H3K9/K27JMJD1A
H3K4me1-2SET7/9 (peptide)
SET7/9 
(full-length histone)
H3K9me2G9aJMJD2A
H3K27me2-1EZH2JMJD3
H3K27me3 
H3K36me2JMJD2A
H3K79me2DOT1L
H3R2mePRMT6
H3/H4Rme panPRMT4
Histone H4H4R3mePRMT1
H4K20me1SET8

Products

AlphaLISA® 5X Epigenetics Buffer 1

The two-component AlphaLISA® 5X Epigenetics Buffer 1 kit is specifically formulated for the detection...  View more

AlphaLISA® Anti-acetyl-Histone H4 Lysine (H4Kac pan) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H4 acetylated lysine residues (H4Kac pan)...  View more

AlphaLISA® Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is acetylated at lysine 27 (H3K27ac)...  View more

AlphaLISA® Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human p53 that is acetylated at lysine 382 (p53K382ac) ...  View more

AlphaLISA® Anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is mono-methylated or di-methylated...  View more

AlphaLISA® Anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is di-methylated at lysine 36 (H3K36me2)...  View more

AlphaLISA® anti-Histone H3 (C-term) Acceptor Beads

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L....  View more

AlphaLISA® Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is methylated at arginine 2(H3R2me)...  View more

AlphaLISA® Anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 and H4 methylated arginine residues (H3/H4Rme...  View more

AlphaLISA® Anti-methyl-Histone H4 Arginine 3 Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H4 methylated at arginine 3 (H4R3me) in a...  View more

AlphaLISA® Anti-mono-methyl-Histone H4 Lysine 20 Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H4 that is mono-methylated at lysine 20(H4K20me1)...  View more

AlphaLISA® Anti-O-linked GlcNAc Acceptor Beads

AlphaLISA® Acceptor beads designed to detect O-linked n-acetylglucosamine (O-linked GlcNAc) in a homogeneous...  View more

AlphaLISA® Anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is tri-methylated at lysine 27 (H3K27me3)...  View more

AlphaLISA® Anti-unmodified Histone H3 Lysine 4 (H3K4) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is unmodified (H3K4) in a homogeneous...  View more

AlphaLISA® Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is unmodified at lysine 9 and lysine...  View more

AlphaLISA® Biotin-anti-H3K79me2 Antibody

AlphaLISA® antibody designed to detect human Histone H3 that is dimethylated at lysine 79 (H3K79me2)...  View more

AlphaLISA® Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. ...  View more

AlphaLISA® Biotinylated Anti-Histone H3 (C-ter) Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the carboxy-terminal end of human histone H3....  View more

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 acetylated at lysine 9 (H3K9ac) in a ...  View more

Anti-methyl-Histone H3 Lysine 4 (H3K4me1-2) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is mono-methylated or di-methylated...  View more

Anti-methyl-Histone H3 Lysine 9 (H3K9me2) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads designed to detect human Histone H3 that is di-methylated at lysine 9 (H3K9me2)...  View more

LANCE® TR-FRET Epigenetics Tool Box

Consistent, validated solutions for your most demanding epigenetics applications. - Proven, cost-efficient...  View more